Cryo-electron tomography generates 3D structures of biological samples by recording a series of 2D images at various angles and then back-projecting them into a reconstruction. Thermo Scientific Tomography 4 Software provides easy and efficient retrieval of high-quality sample data for reliable structural reconstruction.

With Tomography 4 Software, the acquisition process is simplified with automation of key workflow elements along with on-the-fly reconstruction during the experiment. This allows for ongoing visualization of the reconstructed volume. Together, these enhancements significantly improve throughput and reconstruction quality, enabling you to get the information you need faster and more easily.

Software for Electron Microscopes

3D TEM Reconstruction with Tomography 4 Software

3D electron tomography solution for structural analysis of materials and biological samples.


Key Features

  • On-the-fly reconstruction algorithm – Quality assessment during acquisition enables higher yield and reduced time to data.
  • Easy graphical setup for low-dose specimens. Minimizes damage not only for single acquisitions, but also in batch mode.
  • Fully automatic TEM and STEM acquisition of multiple signals for life science and materials science applications.
  • Easy navigation for batch setup, allowing you to define all positions for automatic data acquisition.
  • Grid atlas acquisition provides an overview of the entire specimen for fast navigation.
  • One-time calibration – Common calibrations between Tomography and Thermo Scientific EPU Software enable easy, time-efficient operation.
  • Easy workflow from data to structure with Thermo Scientific Inspect 3D Software, EPU Software and tailored data mining with scripting.
Illustration of tomography reconstruction from 2D images to 3D structure.
Back-projection of individual 2D images (left) taken at different tilt angles during a tomography experiment with final reconstruction on the right. Image courtesy of MPI Martinsried, Germany.

Specifications

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Packages

New instruments

1050133 TEM Tomography Data Acquisition 4
1050137 STEM Tomography Data Acquisition 4

Field upgrades

1059420 TEM Tomography Data Acquisition 4
1059422 STEM Tomography Data Acquisition 4
Tomography approved holders Tomography Holder FP 5341/00
Tomography Holder (High Field-of-View) 9432 909 97191
Talos Arctica Cryo-TEM
Krios Cryo-TEM
Software requirements Minimum platform software version Tecnai 4.6.2, Talos 1.0.0, Titan 1.6.2 (Windows XP) or Titan 2.0.0 (Windows 7)
STEM Tomography Data Acquisition 4 Upgrade  
Software 1050133 (TEM Tomography Data Acquisition Software)
1059420 (TEM Tomography Data Acquisition 4 Upgrade)
Hardware requirements STEM hardware, including an HAADF or on-axis bright-field/dark-field detector
Other requirements For Tecnai instruments: PIA scan generator (National Instruments is not supported)

Applications

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Infectious Disease Research

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

Plant_Biology_Research_Application_274x180_144_DPI

Plant Biology Research

Fundamental plant biology research is enabled by cryo electron microscopy, which provides information on proteins (with single particle analysis), to their cellular context (with tomography), all the way up to the overall structure of the plant (large volume analysis).

Pathology_Pancreas_274x180

Pathology Research

Transmission electron microscopy (TEM) is used when the nature of the disease cannot be established via alternative methods. With nano-biological imaging, TEM provides accurate and reliable insight for certain pathologies.

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Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

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