Antibody Drug Conjugates (ADCs)

Simplify antibody drug conjugate analysis characterization and drug-antibody ratio calculations

An antibody drug conjugate (ADC) is a protein — typically a monoclonal antibody (mAb) — covalently bound (conjugated) to a small-molecule drug using an ADC linker. Combining the targeting capabilities of a mAb with a potent cancer-killing drug allows sensitive discrimination between healthy and diseased cells. Development of these biotherapeutics is more difficult than development of mAbs alone due to the potency of the conjugated drug.

Our powerful technologies, including specific UHPLC systems, mass spectrometry, and LC columns designed for detailed ADC characterization, make separation and characterization of these novel molecules easier.

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Popular antibody drug conjugate analysis products

Drug-antibody ratio (DAR) workflow

Drug antibody ratio workflow

Antibody drug conjugates categories

Hydrophobic interaction chromatography for determining drug load distribution

Separate mAbs and ADCs using Thermo Scientific MAbPac HIC-Butyl HPLC columns. Based on hydrophilic, nonporous, 5μm polymer particles. The latter functionalized with butyl groups. Hydrophilic resin provides excellent biocompatibility and low carryover, while the optimal density of the butyl groups provides high-resolution separation. The MAbPac HIC-Butyl column is one of our first HPLC columns designed and engineered specifically with antibody drug conjugates in mind.

Alternatively, use Thermo Scientific MAbPac SEC-1 Size Exclusion Columns for high-resolution separation of mAb monomers, aggregates and Fab and Fc fragments resulting from proteolysis. These size-exclusion chromatography (SEC) columns provide superior performance, even under non-denaturing conditions, and in high- and low-salt mobile phases and volatile eluents. Perfect for ADCs.

Say goodbye to your QTOF for native ADC mass spectrometry

The new Thermo Scientific Orbitrap Exploris 480 Mass Spectrometer now adds superior denatured and native MS intact analysis and subunit top/middle-down analysis capabilities to the most powerful compact peptide mapping instruments available. High Mass Range (HMR) mode enables increased scanning range up to 8,000 m/z, providing the flexibility to analyze your intact proteins in either denaturing or native MS conditions. Now the gold standard in peptide mapping excels in intact mass analysis too.

Compliant LC & LC-MS software

Streamline your laboratory workflow using Thermo Scientific Chromeleon 7.3 Chromatography Data System (CDS) software. This software delivers superior instrument control, automated DAR calculation, and data processing for compliant GxP biopharmaceutical ADC manufacturing and QA/QC environments.

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Intact and Native Characterization webinars
Intact and Native Characterization webinars

Join experts from Genentech and Thermo Fisher to get great structural insight into your drug product using Orbitrap high resolution accurate mass spectrometers.