Perform unequivocal glycoform analysis
Among the analytical tools used for the analysis of protein glycosylation, high resolution accurate mass (HRAM) mass spectrometry (MS) has become increasingly important for the determination and quantification of the various glycoforms. New methods coupling capillary UHPLC with Orbitrap-based mass spectrometry give rapid glycoform analysis for direct comparison of biotherapeutic products during discovery, development, and QC.
Intact glycoform analysis workflow
The new Thermo Scientific Orbitrap Exploris 240 MS of mass spectrometers now add superior denatured and native MS intact analysis and subunit top/middle-down analysis capabilities to the most powerful benchtop peptide mapping instruments available. High Mass Range (HMR) mode enables increased scanning range up to 8,000 m/z, providing the flexibility to analyze your intact proteins in either denaturing or native MS conditions. Now the gold standard in peptide mapping excels in intact mass analysis too.
Perform high-performance, reversed-phase (RP) LC and LC-MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins using the new Thermo Scientific™ MAbPac™ RP columns. These columns have excellent resolution, long column lifetime, and low carry over. The surface chemistry, particle size, and pore size, have been optimized to for high-resolution separations.
Screen, identify, and characterize intact proteins with higher productivity and confidence using the intact glycoprotein analysis workflow in BioPharma Finder software. Two deconvolution algorithms take full advantage of the high-quality HRAM data produced by Thermo Scientific Orbitrap mass spectrometers. In addition to intact characterization, you can confirm amino acid sequence, identify site and type of known/unknown post translational modifications, while providing relative amounts using Peptide Mapping workflows. Additional features include disulfide linkage, sequence variant analysis, and sequence alterations.
New Thermo Scientific™ Vanquish™ Flex UHPLC delivers confidence, with a fully bio-compatible flow path and proven compliance. Achieve unprecedented performance in retention time stability, sensitivity and separation efficiency with the widest range of column chemistries for biotherapeutic protein characterization. Demand versatility through seamless integration with market-leading mass spectrometry, fluorescence and charged aerosol detection. Gain operational simplicity with easy, freely available, one-click workflows via AppsLab and tool-free Thermo Scientific™ Viper™ fittings.
Biotherapeutic proteins, which include antibodies, are becoming the fastest growing category of drugs due to their efficacy for patient health. The complex nature of these large molecules brings major new challenges to the pharmaceutical scientist. Read about the series of four simple workflow solutions we've developed, based on leading technology platforms.