Glycopeptide Workflow

Site specific glycan analysis

Regulatory agencies have placed increased pressure on biopharmaceutical manufacturers to demonstrate satisfactory programs for understanding, measuring, and controlling glycosylation in glycoprotein-based drugs, with recent updated guidance from the US FDA for characterization of biosimilar therapies.

The Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer offers unique acquisition strategies and workflows through its flexibility in fragmentation types and MSn capabilities that enable the user to go beyond of what is possible in glycan and glycopeptides analyses. Glycan analysis performed at the peptide level provides both glycan composition and peptide sequence at the site of glycosylation. 

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Popular glycopeptide workflow products
Orbitrap Eclipse Mass Spectrometer

Glycopeptide workflow

glycopeptide workflow

Glycopeptide workflow categories

Reproducible, easy-to-use UHPLC

The new Thermo Scientific Vanquish Flex UHPLC platform offers the ultimate in biocompatible state-of-the-art binary or quaternary solvent blending. The Vanquish Flex platform is designed to deliver new benchmarks in accuracy, precision, and sensitivity in peptide chromatography.  With rock steady retention times, you can be confident in the identity of every peptide in discovery, development and QC environments.

Seamless compatibility with both DAD, VWD, and mass spectrometric detection and fully compliant Thermo Scientific Chromeleon CDS software makes this the UHPLC system of choice for your entire drug pipeline.

Fragmentation versatility for complete glycopeptide characterization

Determine the exact location of glycosylation with advanced fragmentation techniques on the Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer. The availability of multiple fragmentation techniques - CID, HCD, ETD; combined ionization spectra such as EThcD; and also product dependent acquisition (such as HCDpdETD), which generates a pair of HCD and ETD spectra. At any stage of MSn, with fragment ion detection in either the ion trap or Orbitrap mass analyzer, offers a new level of versatility and performance for the most challenging applications. Such as, the identification and location of glycopeptides.

BioPharma Finder 3.0 Software
Integrated bottom-up analysis and intact mass characterization

Save time and identify more with BioPharma Finder integrated software. Simple software workflows guide you along the biotherapeutic characterization pathway, providing full coverage including peptide sequence verification, identification and relative quantification of all variants and modifications; including site-specific glycopeptide identification.

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Join the sweet revolution in biopharmaceuticals – Download glycan analysis brochure
Join the sweet revolution in biopharmaceuticals

Biotherapeutic proteins, which include antibodies, are becoming the fastest growing category of drugs due to their efficacy for patient health. The complex nature of these large molecules brings major new challenges to the pharmaceutical scientist.