One system, maximum insights
Today’s cutting-edge research pushes LC-MS to its limits. Obtaining high confidence insights to enable accurate resolution of subtle differences and avoid costly dead-ends is needed faster than ever before. The Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer surpasses these limits with new innovations that deliver the ultimate flexibility to expand experimental scope, and with built-in intelligence to ensure the highest data quality and confidence. One system provides maximum insights, so you productively go beyond today’s discovery.
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Dr. Ryan Kelly discusses how to optimize and minimize sample loss in single cell sample preparation for MS analysis plus covers best practices for single cell analysis with LC-MS, including using isobaric multiplexing strategies such as TMT and optimizing your mass spec system with FAIMS technology.
Key benefits of the Orbitrap Eclipse Tribrid mass spectrometer
|Power high impact science with performance advancements||Gain ultimate flexibility with the ability to expand your experimental scope||Obtain highest quality MS data with built-in intelligence||Improve your laboratory productivity with a single system that provides maximum insights|
|Address complex challenges more effectively with the latest improvements in calibration, ion transmission efficiency, mass range, data acquisition speed and much more!||You need LC-MS workflows to push today’s limits. The Orbitrap Eclipse Tribrid MS comes with enhanced sensitivity, selectivity and more MSn techniques, scan modes and intelligence. It’s now possible to go beyond your scientific pursuits with the ability to expand your experimental scope.||The Orbitrap Eclipse Tribrid MS eliminates convoluted optimization procedures by providing pre-defined data acquisition methods, pre-set instrument parameters and built-in intelligence for workflows including TMT. No compromises on data quality needed.||The ability to go beyond today’s discovery is now possible with one mass spectrometer built to solve analytical challenges for proteomics, structural biology, small molecule research and biopharmaceutical services.|
Learn how the Orbitrap Eclipse Tribrid mass spectrometer works
See what makes the Orbitrap Eclipse Tribrid MS able to fuel your next discovery, and learn how it can empower more users to make new discoveries.
New mass analyzer, new experimental possibilities provide you with what you need today but also the ability to build onto your discoveries in the future. With resolution up to 500,000 (FWHM) at m/z 200, an option for 1 Million (1M) resolution setting to remove interferences, an increased mass range up to m/z 8000 (HMRn) and improved scan speed up to 40Hz for MS and MSn, these innovations help you surpass discovery limitations.
Orbitrap Eclipse Tribrid MS ion path
Synchronous Precursor Selection (SPS) enables improved specificity, significantly increasing analytical performance of TMT-based quantification experiments. Applying Real-Time Search data acquisition strategy with the SPS MS3 method enables the comprehensive characterization of at least 10-plex proteome in 50% less acquisition time by leveraging the MS2 spectrum and Real-Time Search data acquisition to determine which precursors are further analyzed by SPS MS3, increasing the efficiency and confidence of data acquisition. Data indicates that the Real-Time Search-aided TMT SPS MS3 workflow provides dramatic performance improvements for quantitative multiplexed experiments.*
Real-Time Search data acquisition improves experimental speed and quantitation accuracy
Real-Time Search improves experimental speed and quantification accuracy when combined with the advanced SPS MS3 experimental approach. With Real-Time Search data acquisition, MS3 scans are only triggered if a peptide-spectrum match (PSM) is identified from the preceding MS2. This reduces the number of MS3 events, increases the precursor sampling rate and results in a greater number of quantified peptides; improving experimental speed. With the aid of the Real-Time Search data acquisition strategy, the Orbitrap Eclipse Tribrid MS selects only those fragment ions that correspond to the identified PSM for quantification in the MS3 scan, resulting in improved quantitation accuracy.
Erickson et al. J Proteome Res. 2019 Mar 1;18(3):1299-1306
Thermo Scientific SureQuant IS Targeted Protein Quantitation workflow is a data-aware quantitation scan mode leveraging internal standards to dynamically adjust scan parameters and automatically maximize data quality for targeted proteome analysis in real time. The SureQuant method fills the gap for large-scale target profiling with superior quantitative performance.
SureQuant IS targeted protein quantitation
While triple quadrupole-based Selected Reaction Monitoring (SRM) and high-resolution Parallel Reaction Monitoring (PRM) approaches are different, each offers advantages to targeted quantitation. SureQuant IS targeted protein quantitation with Thermo Scientific Orbitrap mass analyzer-based mass spectrometry reduces the inevitable trade-off between target multiplexing, sensitivity and selectivity. This method enlarges targeted protein panel sizes and significantly improves limits of detection (LODs) and quantitative accuracy without use of retention time windows.
SureQuant method facilitates an intelligence-driven approach
SureQuant method target detection occurs independently of Retention Time (RT) scheduling, allowing reliable and robust target quantification. This intelligence-driven approach for quantitation enables self-adjustment for higher sensitivity without compromising cycle time.
Designed to improve the MSn acquisition rates for TMT experiments while maintaining sufficient MS2 or MS3 resolution to differentiate and quantitate isobaric reporter ions. Increase experimental performance for higher order TMT multiplexing without sacrificing acquisition speed using shorter gradients for higher throughput.
Active Ion Management (AIM+)
Maximizes ion transmission to achieve unprecedented levels of qualitative and quantitative performance
Enhanced Vacuum Technology
Improves Orbitrap mass analyzer performance for everyday proteomics applications and more
Improved accumulation and transmission of high molecular weight ions to the Orbitrap mass analyzer
Enables better sensitivity (improved signal/noise ratio) and performs better mass measurements of intact proteins at higher resolution
Precursor Fit filtering
Precursor Fit filter allows for selecting ions based on precursor ion specificity of the isolation window.
Setting up your system to acquire the highest quality data is simple and more effortless with a consistent, common instrument control software approach covering our Thermo Scientific Orbitrap Tribrid MS, Thermo Scientific Orbitrap Exploris 480 MS, and Thermo Scientific TSQ triple quadrupole mass spectrometers.
Focus on your science, rather than experimental set-up, through ready-to-use application specific method templates for common experiments; method development can be eliminated or significantly reduced through using these templates, editing them or creating your own through drag-and-drop method set-up.
Generating high-quality data is easier than ever before with drag-and-drop method-building and the ability to utilize ready-made templates for the most popular experiments. The common software architecture provides a consistent user experience, simplifying operation, training and streamlining transfer of knowledge throughout your workflows.
The Thermo Scientific Method Editor and Tune ecosystem deliver intuitive method programming with ready-to-go templates for the most popular experiments, such as Glycosylation, Metabolites, Peptide ID, Peptide Quantitation, PTM, Advanced TMT, SureQuant method and more.
The common method editor combines both instrument control and data acquisition parameters, through the use of extensive templates for the most common experiments to allow you to acquire high-quality data from the start, or use drag-and-drop to rapidly create and visualize your own experiments.
Intelligence – driven
Thermo Scientific AcquireX data acquisition provides all-inclusive, automated small molecule sample profiling on an LC-timescale. Dynamic Scan Management enables intelligent, real-time scheduling, parallelization, and prioritization of the acquisition events as well as selection, sorting, and routing of precursors to different fragmentation modes and analyzers based on user-selected parameters, including precursor m/z, intensity, and/or charge. An intuitive, flexible drag-and-drop instrument method editor interface simplifies development and enables unique, complex workflows.
Optional features to maximize performance
Maximize performance and flexibility to expand experimental capabilities of the Orbitrap Eclipse Tribrid mass spectrometer with multiple hardware and software options, allowing you to see more, quantify more, deliver more confidence and greater insights with even more productivity.
Thermo Scientific FAIMS Pro interface enhances precursor selectivity, improving qualitative and quantitative results for most peptide and protein applications while reducing time consuming sample preparation steps.
Identifying and characterizing proteins and post-translational modifications by bottom-up mass spectrometry relies on the acquisition of high-quality MS and MS/MS data. The FAIMS Pro interface increases analytical performance through gas phase fractionation and selective enhancement of peptidic compounds, reducing the complexity of the MS spectra, and improving analyte signal/noise ratio. The end result is greater proteome coverage.
Achieve unambiguous characterization of lipids, modified peptides and intact proteins, even in complex mixtures, and obtain unique structurally diagnostic information (e.g., double bonds) that cannot be obtained with CID, HCD, ETD or EThcD. UVPD enhances unique sequence coverage over 20%, generating MSn fragments in the linear ion trap that can be detected by either the ion trap or Orbitrap mass analyzer.
Leverages Extended Mass Range (EMR)-improved resolution and sensitivity of large proteins and simplifies MSn spectra of multiply charged analytes for increased confidence in intact and middle-down protein sequencing. PTCR simplifies complex spectra making them deconvolutable.
Improve mass-to-charge (m/z) ratio assignment accuracy to under 1 ppm with the Thermo Scientific Easy-IC ion source, which incorporates a secondary ion source that delivers a regulated number of calibrant ions into the analyte ions. This feature enables real-time fine adjustment of the instrument's m/z calibration, correcting otherwise uncompensated errors due to temperature fluctuations and scan-to-scan variations.
Thermo Scientific 1 Million (M) resolution
Resolve isobaric components, obtain fine isotope information, and achieve confident assignment of the elemental composition of small molecule analytes with the ultra-high 1 Million (1M) resolution setting of the Orbitrap Eclipse Tribrid Mass Spectrometer. The 1M resolution option significantly aids the characterization and quantitation of structurally diverse targets by obtaining mass measurements at ultra-high resolution (1,000,000 FWHM at m/z 200) for structurally diverse targets.
A mass spectrum of Irganox 1035 acquired at the 1,000,000 (1M) resolution setting (m/z 200) with a 2 s transient. The right panel shows the fine structure for the A+2 isotope acquired at the 500,000 versus 1,000,000 resolution setting. The 18O and 213C isotopes can be resolved using the Orbitrap Fusion Lumos with 1M.
What customers are saying:
Provides expanded mass range capabilities for comprehensive MSn characterization of intact proteins and protein complexes.
Proteomics research requires more than just simple identification. Thermo Scientific Proteome Discoverer software offers a full suite of analysis tools with the flexibility to address multiple research workflows, and an easy-to-use, wizard-driven interface. Confidently interpret your data with sophisticated statistical and visualization tools unique to Proteome Discoverer software, and utilize third-party algorithms to take your analysis to the next level.
Recommended for the following workflows:
- Iterative search strategies
- Multi-search engine comparison
- TMT SPS MS3
- Glycopeptide identification and quantification
- Phosphopeptide ID and quantification
- Top-down proteomics
Complete protein characterization can be challenging, whether you are performing intact protein mass analysis, top- and middle-down analysis, peptide mapping or multi-attribute method (MAM) workflows. Access workflows to facilitate comprehensive interpretation and data visualization, confidently characterizing your biologics with speed and ease. Thermo Scientific BioPharma Finder software helps you choose the right path for confident protein characterization.
Recommended for the following workflows:
- Intact analysis
- Top-down and middle-down analysis
- Peptide mapping
- Multi-attribute method (MAM)
Monitor your mass spectrometry analyses anywhere, anytime.
Using the Thermo Scientific Almanac web- and smartphone-based application gives you the ability to remotely access and manage your LC-MS systems, freeing you to focus on other important tasks during your day.
Check real-time system status and acquisitions, set up automated e-mails to notify you of a completed acquisition or error, schedule instrument access, monitor utilization, or send service files to aid in system diagnostics and maximize up-time.
Key Almanac Tools
- Instrument Connect Platform: manage your systems and data in a single location
- Instrument Status: see what is happening in real time
- Instrument Logbook and Events: easily see what has happened and log important information
- Instrument Utilization Reports: evaluate system usage over time
- Instrument Scheduler: manage system access and utilization
- Push to Service: minimize time taken to diagnose issues
Connect your laboratory: Always know what’s going on within your laboratory with any cloud-connected device. Maximize system utilization with long-term trending of system usage and sample throughput, through to checking data acquisition and multiple other productivity tools.
Target applications for Obitrap Eclipse Tribrid mass spectrometer
Orbitrap Eclipse Tribrid MS ordering guides
Recommended configuration for biopharma
Recommended configuration for proteomics
Orbitrap Eclipse Tribrid mass spectrometer ordering guide
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Expand your application knowledge
Our LC-MS on-demand webinar series expands your application knowledge for pharma, biopharma, environmental, omics, food, forensics and clinical research and more. Learn how the latest high-performance mass spectrometry solutions can enable your laboratory to solve your most pressing analytical challenges, regardless of sample type.