Orbitrap LC-MS Comparison Chart

Choose from an extensive portfolio of high-resolution accurate-mass (HRAM) Thermo Scientific Orbitrap mass analyzers. Our Orbitrap-based MS systems connect to liquid chromatography equipment to enhance separation of unknown compounds and enable high-throughput workflows. Some of our Orbitrap mass analyzers combine with ion traps and quadrupole mass filters. Compare products below to find the Orbitrap LC-MS instruments that meet your analysis needs the best.

Hybrid Orbitrap Mass Spectrometry

Choose the high-resolution accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your lab

  Q Exactive Focus Q Exactive Q Exactive Plus
  Q Exactive
Focus
Q Exactive
 
Q Exactive
Plus
Ideal Applications Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Targeted Metabolomics Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Targeted Metabolomics Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Sport Anti-Doping, Biopharma development, Metabolomics, Lipidomics
Analyses MS and MS/MS, Qual/Quan, Screening & Confirmation
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
Sample thoughtput Low to medium Medium to high Medium to high
Standard Features
Resolving Power @ m/z 200 70,000 140,000 140,000
(up to 280,000 with BioPharma option)
Mass Accuracy, (Internal Callibration) <1 ppm with internal calibration
Mass Range m/z 50 - 3,000 m/z 50 - 6,000
(up to 8000 with option)
Scan Speed Up to 12 Hz
Dynamic Range Up to 5 orders
Ion Source IonMax with H-ESI II probe
Polarity Switching 1 full cycle <1 sec at 35,000 resolution setting
Multiplexing 3 (SIM only) 10
Scan Functions SIM SIM & PRM
Dissociation In-source CID, HCD
Optional Upgrades
Intact Protein Mode N/A Yes
Enhanced Resolution Mode N/A Yes
Performance and Value
High Perfomance Hybrid Orbitrap Mass Spectrometer

Choose the high performance, high-resolution accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your translational research lab

  Orbitrap Exploris 480 Q Exactive HF-X Q Exactive HF Q Exactive UHMR
  Orbitrap Exploris 480 Q Exactive
HF-X
Q Exactive
HF
Q Exactive
UHMR
Ideal Applications Proteomics, BioPharma Proteomics, BioPharma, Metabolomics Proteomics, Structural Biology
Analyses MS and MS/MS, Qual/Quan, Screening & Confirmation MS and MS/MS, Screening & Confirmation
Advanced Data-Dependent Experiments SureQuant, Precursor Fit, Boxcar Scanning N/A
Almanac Web-Based Monitoring and Management Require Foundation 3.1 SP1 or later
Standard Features
Resolving Power 480,000 at m/z 195 240,000 at m/z 200 120,000 at m/z 200
(up to 240,000 with option)
200,000 at m/z 400
Mass Accuracy
(Internal Callibration)
<1 ppm internal calibration
Mass Range m/z 40 - 6000
(up to 8000 with BioPharma option)
m/z 50 - 6,000
(up to 8000 with BioPharma option)
m/z 350 - 80,000
Scan Speed Up to 18 Hz Up to 40 Hz Up to 18 Hz Up to 12 Hz (at resolution setting of 12,500 @ m/z 400
Ion Source (1 full cycle <1 sec)
Polarity Switching OptaMax NG IonMax with H-ESI II probe
Multiplexing Up to 20 precursors/scan N/A
Scan Functions FS, AIF, SIM, t-MS2, DIA, ddHCD FS, AIF, SIM, PRM, DIA, ddHCD FS, AIF, MS/MS, pseudo-MS3
Dissociation In-source CID, IRM In-source CID, HCD In-source trapping, HCD
Optional Upgrades
Intact Protein Mode Yes
High Mass Rangen(HMRn, up to 8000 m/z) Yes N/A
Easy-IC Ion Source Yes N/A
FAIMS Pro Interface Yes N/A
Performance and Value
Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

Learn more  Watch free webinar

Go beyond with Orbitrap Tribrid MS for large and small molecule analysis

Choose the maximum performance Tribrid Orbitrap mass spectrometer that's right for your discovery lab

  Orbitrap ID-X Orbitrap Fusion Lumos Orbitrap Eclipse
  Orbitrap ID-X Tribrid MS Orbitrap Fusion Lumos Tribrid MS Orbitrap Eclipse Tribrid MS
Ideal Applications Small molecule characterization and elucidation research for applications such as Metabolomics, Drug development Met ID, Extactables and Leachables, Industrial Environmental Health and more Multi-omics, TMT, Intact/top-down proteomics, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking Multi-omics, TMT, Intact/top-down proteomics, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking, NativeOmics, LC-top-down mass spectrometry, Biotherapeutics
Analyses MS and MSn, Qualitative and Quantitative, Profiling, screening, and targeted consistently cap or uncap
Advanced Data-Dependent Experiments Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, MSn Library builder SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quanfirmation Acquisition, SureQuant RTS SPS MS3 ,Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quanfirmation Acquisition, SureQuant, Precursor FitFilter
AcquireX Acquisition Tool Requires Thermo Scientific Orbitrap Tribrid Series 4.2 SP1QF1, 3.1 or greater
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
Standard Features
Resolution (FWHM) @m/z 200 7,500-500,000 7,500-500,000
(1M optional)
Mass Accuracy (internal callibration) <3 ppm (<1ppm)
Mass Range m/z 50 - 2,000 m/z 50 - 6,000 m/z 50 - 6,000
(up to 8000 with HMRn)
Ion Source OptaMax NG
Ion Optics S-lens High-Capacity Ion Transfer Tube (HCTT), Electrodynamic Ion Funnel (EDIF)
Scan Speed (Under Defined Conditions) 30 Hz (OT) 40 Hz (IT) 40 Hz (OT) 45 Hz (IT)
Polarity Switching One full cycle in 1.1 sec (positive and negative full scan with resolution of 30,000)
Scan Functions Full scan, MS/MS, MSn, SIM, PRM, DIA, AIF Full scan, MS/MS, MSn, SIM, PRM, DIA, AIF, TurboTMT
MSn Level Up to 10
Minimum Quadrupole Mass Filter 0.4 amu
Linear Ion Trap Isolation 0.2 to 600 amu 0.2 to 4000 amu
Dissociation In-source CID, HCD
Optional Upgrades
ETD/EThcD No EASY-ETD
UVPD Laser No Yes
High Mass Rangen (HMRn, up to 8,000 m/z) No Yes
Proton Transfer Charged Reactions (PTCR) No Yes
EASY-IC Ion Source Yes
1M Resolution No Yes
FAIMS Pro Interface No Yes
Performance and Value
Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

Learn more  Watch free webinar

For more information

Hybrid Orbitrap Mass Spectrometry

Choose the high-resolution accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your lab

  Q Exactive Focus Q Exactive Q Exactive Plus
  Q Exactive
Focus
Q Exactive
 
Q Exactive
Plus
Ideal Applications Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Targeted Metabolomics Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Targeted Metabolomics Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Sport Anti-Doping, Biopharma development, Metabolomics, Lipidomics
Analyses MS and MS/MS, Qual/Quan, Screening & Confirmation
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
Sample thoughtput Low to medium Medium to high Medium to high
Standard Features
Resolving Power @ m/z 200 70,000 140,000 140,000
(up to 280,000 with BioPharma option)
Mass Accuracy, (Internal Callibration) <1 ppm with internal calibration
Mass Range m/z 50 - 3,000 m/z 50 - 6,000
(up to 8000 with option)
Scan Speed Up to 12 Hz
Dynamic Range Up to 5 orders
Ion Source IonMax with H-ESI II probe
Polarity Switching 1 full cycle <1 sec at 35,000 resolution setting
Multiplexing 3 (SIM only) 10
Scan Functions SIM SIM & PRM
Dissociation In-source CID, HCD
Optional Upgrades
Intact Protein Mode N/A Yes
Enhanced Resolution Mode N/A Yes
Performance and Value
High Perfomance Hybrid Orbitrap Mass Spectrometer

Choose the high performance, high-resolution accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your translational research lab

  Orbitrap Exploris 480 Q Exactive HF-X Q Exactive HF Q Exactive UHMR
  Orbitrap Exploris 480 Q Exactive
HF-X
Q Exactive
HF
Q Exactive
UHMR
Ideal Applications Proteomics, BioPharma Proteomics, BioPharma, Metabolomics Proteomics, Structural Biology
Analyses MS and MS/MS, Qual/Quan, Screening & Confirmation MS and MS/MS, Screening & Confirmation
Advanced Data-Dependent Experiments SureQuant, Precursor Fit, Boxcar Scanning N/A
Almanac Web-Based Monitoring and Management Require Foundation 3.1 SP1 or later
Standard Features
Resolving Power 480,000 at m/z 195 240,000 at m/z 200 120,000 at m/z 200
(up to 240,000 with option)
200,000 at m/z 400
Mass Accuracy
(Internal Callibration)
<1 ppm internal calibration
Mass Range m/z 40 - 6000
(up to 8000 with BioPharma option)
m/z 50 - 6,000
(up to 8000 with BioPharma option)
m/z 350 - 80,000
Scan Speed Up to 18 Hz Up to 40 Hz Up to 18 Hz Up to 12 Hz (at resolution setting of 12,500 @ m/z 400
Ion Source (1 full cycle <1 sec)
Polarity Switching OptaMax NG IonMax with H-ESI II probe
Multiplexing Up to 20 precursors/scan N/A
Scan Functions FS, AIF, SIM, t-MS2, DIA, ddHCD FS, AIF, SIM, PRM, DIA, ddHCD FS, AIF, MS/MS, pseudo-MS3
Dissociation In-source CID, IRM In-source CID, HCD In-source trapping, HCD
Optional Upgrades
Intact Protein Mode Yes
High Mass Rangen(HMRn, up to 8000 m/z) Yes N/A
Easy-IC Ion Source Yes N/A
FAIMS Pro Interface Yes N/A
Performance and Value
Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

Learn more  Watch free webinar

Go beyond with Orbitrap Tribrid MS for large and small molecule analysis

Choose the maximum performance Tribrid Orbitrap mass spectrometer that's right for your discovery lab

  Orbitrap ID-X Orbitrap Fusion Lumos Orbitrap Eclipse
  Orbitrap ID-X Tribrid MS Orbitrap Fusion Lumos Tribrid MS Orbitrap Eclipse Tribrid MS
Ideal Applications Small molecule characterization and elucidation research for applications such as Metabolomics, Drug development Met ID, Extactables and Leachables, Industrial Environmental Health and more Multi-omics, TMT, Intact/top-down proteomics, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking Multi-omics, TMT, Intact/top-down proteomics, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking, NativeOmics, LC-top-down mass spectrometry, Biotherapeutics
Analyses MS and MSn, Qualitative and Quantitative, Profiling, screening, and targeted consistently cap or uncap
Advanced Data-Dependent Experiments Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, MSn Library builder SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quanfirmation Acquisition, SureQuant RTS SPS MS3 ,Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quanfirmation Acquisition, SureQuant, Precursor FitFilter
AcquireX Acquisition Tool Requires Thermo Scientific Orbitrap Tribrid Series 4.2 SP1QF1, 3.1 or greater
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
Standard Features
Resolution (FWHM) @m/z 200 7,500-500,000 7,500-500,000
(1M optional)
Mass Accuracy (internal callibration) <3 ppm (<1ppm)
Mass Range m/z 50 - 2,000 m/z 50 - 6,000 m/z 50 - 6,000
(up to 8000 with HMRn)
Ion Source OptaMax NG
Ion Optics S-lens High-Capacity Ion Transfer Tube (HCTT), Electrodynamic Ion Funnel (EDIF)
Scan Speed (Under Defined Conditions) 30 Hz (OT) 40 Hz (IT) 40 Hz (OT) 45 Hz (IT)
Polarity Switching One full cycle in 1.1 sec (positive and negative full scan with resolution of 30,000)
Scan Functions Full scan, MS/MS, MSn, SIM, PRM, DIA, AIF Full scan, MS/MS, MSn, SIM, PRM, DIA, AIF, TurboTMT
MSn Level Up to 10
Minimum Quadrupole Mass Filter 0.4 amu
Linear Ion Trap Isolation 0.2 to 600 amu 0.2 to 4000 amu
Dissociation In-source CID, HCD
Optional Upgrades
ETD/EThcD No EASY-ETD
UVPD Laser No Yes
High Mass Rangen (HMRn, up to 8,000 m/z) No Yes
Proton Transfer Charged Reactions (PTCR) No Yes
EASY-IC Ion Source Yes
1M Resolution No Yes
FAIMS Pro Interface No Yes
Performance and Value
Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

Learn more  Watch free webinar

For more information


Definition of terms

Resolution: Frequently reported in full width at half maximum (FWHM), resolution is the separation of two mass spectrometry peaks and is calculated as the mass of a peak (M) divided by resolving power (∆M).

Mass accuracy: Expressed in parts per million, this value indicates how much an instrument deviates from a known standard or calibrant.

Scan rate: Measured in hertz, the scan rate of an MS indicates how quickly it scans a mass spectrum.

Polarity switch: The collection of both positive and negative sample ionization data (spectra) by the ion trap during a single MS analysis. Polarity switching is applied to the study of drugs, drug metabolites, pesticides, etc.

PRM: Parallel reaction monitoring (PRM) is a targeted method of high resolution MS quantification in which a full scan of each precursor ion transition is monitored; i.e., all fragments derived from the precursor ion are monitored. In contrast, single reaction monitoring (SRM) performs one transition monitoring at a time.

Multiplex: The ability of a mass analyzer to acquire data from multiple precursor ions in the same scan without compromising high signal-to-noise ratio.

MSn: Also termed tandem MS, the use of multiple stage MS to capture product ion spectra of a defined m/z, where n is the number of recorded product ion stages.

ETD: Electron transfer dissociation (ETD) results in the fragmentation of the peptide backbone of MS samples, simplifying identification of peptide modification sites.

Synchronous MS3: A method of quantitation that involves simultaneous selection of several MS2 precursors for MS3 analysis and is often used during TMT and iTRAQ experiments.


Featured webinar: structural analysis of ribosome particles by mass spectrometry toolbox for structural biology
Featured webinar: structural analysis of ribosome particles by mass spectrometry toolbox for structural biology

In this symposia Prof. Dr. Albert J.R. Heck discusses new approaches for structural proteomics workflows for crosslinking and native MS.

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