Orbitrap LC-MS Comparison Chart

Choose from an extensive portfolio of Thermo Scientific high-resolution, accurate-mass (HRAM) Orbitrap mass analyzers. Our Orbitrap-based MS systems connect to liquid chromatography instruments to enhance separation of unknown and known compounds and enable high-throughput workflows. Compare products below to find the Thermo Scientific Orbitrap LC-MS instruments that best meet your analysis needs.

Performance Hybrid Orbitrap Mass Spectrometry
Choose a high-resolution, accurate-mass (HRAM) Orbitrap mass spectrometer that offers reliable screening performance with quantitative capability to take on more of your testing requirements that require confident, reportable results.
  Q Exactive Plus Orbitrap Exploris 120
  Q Exactive
Plus MS
Orbitrap
Exploris 120 MS
Ideal Applications Forensic Toxicology, Clinical Research, Food & Environmental Safety Research, Basic Academic Research, Sport Anti-Doping, Biopharma development, Metabolomics, Lipidomics Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Targeted/semi-targeted Metabolomics, Pharmaceuticals
Analyses Unknown identification & confirmation, non-targeted and targeted screening and targeted quantitation
Next-generation ion source interface N/A Yes
Quick start methods for various applications N/A Yes
Drag-n-drop method setup user interface N/A Yes
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
AcquireX Intelligent data acquisition N/A
Standard Features
Resolving Power @ m/z 200 140,000
(up to 280,000 with BioPharma option)
120,000
Mass Accuracy
(Internal Calibration)
<1 ppm with internal calibration <1 ppm for at least 5 days with EASY-IC ion source
Calibration procedures Traditional Calmix One-click calibration with FlexMix calibration solution
Mass Range m/z 50 - 6,000
(up to 8000 with BioPharma option)
m/z 40 - 3,000
Scan Speed 12 Hz 22 Hz
Dynamic Range Up to 5 orders
Ion Source interface IonMax with H-ESI II probe OptaMax NG ion source
Polarity Switching 1 Hz 1.4 Hz
Multiplexing 10 20
Scan Functions Full-MS, AIF, SIM, PRM, (t-MS2 for Orbitrap Exploris 120), DIA
Dissociation In-source CID, HCD
Optional Upgrades
Intact Protein Mode Yes N/A
Enhanced Resolution Mode Yes N/A
Sources APCI/APPI Combination Ion Source
EASY-Spray Sources
Nanospray Flex Ion Sources
APCI/APPI Combination Ion Source
EASY-Spray Sources
Nanospray Flex Ion Sources
High Perfomance Hybrid Orbitrap Mass Spectrometer

Choose the high performance, high-resolution, accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your translational research lab

  Orbitrap Exploris 240 Orbitrap Exploris 480 Q Exactive UHMR
  Orbitrap Exploris 240 Orbitrap Exploris 480 Q Exactive
UHMR
Ideal Applications Forensic Toxicology, Sport Anti-Doping, Food & Environmental Safety research and emerging contaminant testing, Extractables & Leachables, Academic Research, Biopharma development, Semi-targeted Metabolomics, Lipidomics, Clinical & Translational Research Quantitative Proteomics, Protein Identification, Biopharma R&D, Clinical & Translational Research Proteomics, Structural Biology, Protein Characterization
Analyses MS and MS/MS, Qual/Quan, Screening & Confirmation MS and MS/MS, Screening & Confirmation
Advanced Data-Dependent Experiments Precursor Fit SureQuant, Precursor Fit, Boxcar Scanning N/A
Next-generation ion source interface Yes N/A
Quick start methods for various applications Yes N/A
Drag-and-drop method setup user interface Yes N/A
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
AcquireX intelligent data acquisition Yes N/A
Standard Features
Resolving Power @ m/z 200 240,000 480,000 200,000 at m/z 400
Mass Accuracy
(Internal Calibration)
<1 ppm for up to 5 days with EASY-IC ion source <1 ppm internal calibration
Calibration procedures One-click calibration with FlexMix calibration solution Traditional Calmix
EASY-IC Ion Source <1 ppm for up to 5 days N/A
Dynamic Range Up to 5 orders
Mass Range m/z 40 - 6000
(up to 8000 with BioPharma option)
m/z 40 - 6000
(up to 8000 with BioPharma option)
m/z 350 - 80,000
Scan Speed Up to 22 Hz Up to 40 Hz Up to 12 Hz (at resolution setting of 12,500 @ m/z 400
Polarity Switching 1.4 Hz (1 full cycle <1 sec)
Ion Source OptaMax NG ion source IonMax with H-ESI II probe
Multiplexing Up to 20 precursors/scan N/A
Scan Functions FS, AIF, SIM, t-MS2, DIA, ddHCD FS, AIF, MS/MS, pseudo-MS3
Dissociation In-source CID, HCD In-source trapping, HCD
Optional Upgrades
Intact Protein Mode Yes
High Mass Rangen(HMRn, up to 8000 m/z) Yes N/A
Sources FAIMS Pro Interface
APCI/APPI Combination Ion Source
EASY-Spray Sources
Nanospray Flex Ion Sources
VeriSpray PaperSpray Ion Source
APCI/APPI Combination Ion Source
EASY-Spray Sources
Nanospray Flex Ion Sources
Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

Learn more  Watch free webinar

Go beyond with Orbitrap Tribrid MS for large and small molecule analysis

Choose the maximum performance Tribrid Orbitrap mass spectrometer that's right for your discovery lab

  Orbitrap ID-X Orbitrap Fusion Lumos Orbitrap Eclipse
  Orbitrap ID-X Tribrid MS Orbitrap Fusion Lumos Tribrid MS Orbitrap Eclipse Tribrid MS
Ideal Applications Small molecule characterization and elucidation research for applications such as Metabolomics, Drug development Met ID, Extactables and Leachables, Industrial Environmental Health and more Multi-omics, TMT, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking Multi-omics, TMT, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking, NativeOmics, LC-top-down mass spectrometry, Biotherapeutics
Analyses MS and MSn, Qualitative and Quantitative, Profiling, screening, and targeted consistently cap or uncap
Advanced Data-Dependent Experiments Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, MSn Library builder SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quantitation/Confirmation Acquisition, SureQuant Real-Time Search SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quantitation/Confirmation Acquisition, SureQuant, Precursor Fit Filter
AcquireX Acquisition Tool Requires Thermo Scientific Orbitrap Tribrid Series 4.2 SP1QF1, 3.1 or greater
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
Optional Upgrades
EASY-ETD HD No Yes
UVPD Laser No Yes
High Mass Rangen (HMRn, up to 8,000 m/z) No Yes
Proton Transfer Charge Reduction (PTCR) No Yes
EASY-IC Ion Source Yes
1M Resolution No Yes
FAIMS Pro Interface Yes
Performance and Value
Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS technology successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

Learn more  Watch free webinar

For more information

  • Pushing the Boundaries of Protein ID, Characterization & Quantitation
  • Comprehensive Characterization of Proteins Using a Novel Orbitrap Tribrid MS
Performance Hybrid Orbitrap Mass Spectrometry
Choose a high-resolution, accurate-mass (HRAM) Orbitrap mass spectrometer that offers reliable screening performance with quantitative capability to take on more of your testing requirements that require confident, reportable results.
  Q Exactive Plus Orbitrap Exploris 120
  Q Exactive
Plus MS
Orbitrap
Exploris 120 MS
Ideal Applications Forensic Toxicology, Clinical Research, Food & Environmental Safety Research, Basic Academic Research, Sport Anti-Doping, Biopharma development, Metabolomics, Lipidomics Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Targeted/semi-targeted Metabolomics, Pharmaceuticals
Analyses Unknown identification & confirmation, non-targeted and targeted screening and targeted quantitation
Next-generation ion source interface N/A Yes
Quick start methods for various applications N/A Yes
Drag-n-drop method setup user interface N/A Yes
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
AcquireX Intelligent data acquisition N/A
Standard Features
Resolving Power @ m/z 200 140,000
(up to 280,000 with BioPharma option)
120,000
Mass Accuracy
(Internal Calibration)
<1 ppm with internal calibration <1 ppm for at least 5 days with EASY-IC ion source
Calibration procedures Traditional Calmix One-click calibration with FlexMix calibration solution
Mass Range m/z 50 - 6,000
(up to 8000 with BioPharma option)
m/z 40 - 3,000
Scan Speed 12 Hz 22 Hz
Dynamic Range Up to 5 orders
Ion Source interface IonMax with H-ESI II probe OptaMax NG ion source
Polarity Switching 1 Hz 1.4 Hz
Multiplexing 10 20
Scan Functions Full-MS, AIF, SIM, PRM, (t-MS2 for Orbitrap Exploris 120), DIA
Dissociation In-source CID, HCD
Optional Upgrades
Intact Protein Mode Yes N/A
Enhanced Resolution Mode Yes N/A
Sources APCI/APPI Combination Ion Source
EASY-Spray Sources
Nanospray Flex Ion Sources
APCI/APPI Combination Ion Source
EASY-Spray Sources
Nanospray Flex Ion Sources
High Perfomance Hybrid Orbitrap Mass Spectrometer

Choose the high performance, high-resolution, accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your translational research lab

  Orbitrap Exploris 240 Orbitrap Exploris 480 Q Exactive UHMR
  Orbitrap Exploris 240 Orbitrap Exploris 480 Q Exactive
UHMR
Ideal Applications Forensic Toxicology, Sport Anti-Doping, Food & Environmental Safety research and emerging contaminant testing, Extractables & Leachables, Academic Research, Biopharma development, Semi-targeted Metabolomics, Lipidomics, Clinical & Translational Research Quantitative Proteomics, Protein Identification, Biopharma R&D, Clinical & Translational Research Proteomics, Structural Biology, Protein Characterization
Analyses MS and MS/MS, Qual/Quan, Screening & Confirmation MS and MS/MS, Screening & Confirmation
Advanced Data-Dependent Experiments Precursor Fit SureQuant, Precursor Fit, Boxcar Scanning N/A
Next-generation ion source interface Yes N/A
Quick start methods for various applications Yes N/A
Drag-and-drop method setup user interface Yes N/A
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
AcquireX intelligent data acquisition Yes N/A
Standard Features
Resolving Power @ m/z 200 240,000 480,000 200,000 at m/z 400
Mass Accuracy
(Internal Calibration)
<1 ppm for up to 5 days with EASY-IC ion source <1 ppm internal calibration
Calibration procedures One-click calibration with FlexMix calibration solution Traditional Calmix
EASY-IC Ion Source <1 ppm for up to 5 days N/A
Dynamic Range Up to 5 orders
Mass Range m/z 40 - 6000
(up to 8000 with BioPharma option)
m/z 40 - 6000
(up to 8000 with BioPharma option)
m/z 350 - 80,000
Scan Speed Up to 22 Hz Up to 40 Hz Up to 12 Hz (at resolution setting of 12,500 @ m/z 400
Polarity Switching 1.4 Hz (1 full cycle <1 sec)
Ion Source OptaMax NG ion source IonMax with H-ESI II probe
Multiplexing Up to 20 precursors/scan N/A
Scan Functions FS, AIF, SIM, t-MS2, DIA, ddHCD FS, AIF, MS/MS, pseudo-MS3
Dissociation In-source CID, HCD In-source trapping, HCD
Optional Upgrades
Intact Protein Mode Yes
High Mass Rangen(HMRn, up to 8000 m/z) Yes N/A
Sources FAIMS Pro Interface
APCI/APPI Combination Ion Source
EASY-Spray Sources
Nanospray Flex Ion Sources
VeriSpray PaperSpray Ion Source
APCI/APPI Combination Ion Source
EASY-Spray Sources
Nanospray Flex Ion Sources
Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

Learn more  Watch free webinar

Go beyond with Orbitrap Tribrid MS for large and small molecule analysis

Choose the maximum performance Tribrid Orbitrap mass spectrometer that's right for your discovery lab

  Orbitrap ID-X Orbitrap Fusion Lumos Orbitrap Eclipse
  Orbitrap ID-X Tribrid MS Orbitrap Fusion Lumos Tribrid MS Orbitrap Eclipse Tribrid MS
Ideal Applications Small molecule characterization and elucidation research for applications such as Metabolomics, Drug development Met ID, Extactables and Leachables, Industrial Environmental Health and more Multi-omics, TMT, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking Multi-omics, TMT, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking, NativeOmics, LC-top-down mass spectrometry, Biotherapeutics
Analyses MS and MSn, Qualitative and Quantitative, Profiling, screening, and targeted consistently cap or uncap
Advanced Data-Dependent Experiments Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, MSn Library builder SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quantitation/Confirmation Acquisition, SureQuant Real-Time Search SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quantitation/Confirmation Acquisition, SureQuant, Precursor Fit Filter
AcquireX Acquisition Tool Requires Thermo Scientific Orbitrap Tribrid Series 4.2 SP1QF1, 3.1 or greater
Almanac Web-Based Monitoring and Management Requires Foundation 3.1 SP1 or later
Optional Upgrades
EASY-ETD HD No Yes
UVPD Laser No Yes
High Mass Rangen (HMRn, up to 8,000 m/z) No Yes
Proton Transfer Charge Reduction (PTCR) No Yes
EASY-IC Ion Source Yes
1M Resolution No Yes
FAIMS Pro Interface Yes
Performance and Value
Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS technology successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

Learn more  Watch free webinar

For more information

  • Pushing the Boundaries of Protein ID, Characterization & Quantitation
  • Comprehensive Characterization of Proteins Using a Novel Orbitrap Tribrid MS

Definition of terms

Resolution: Frequently reported in full width at half maximum (FWHM), resolution is the separation of two mass spectrometry peaks and is calculated as the mass of a peak (M) divided by resolving power (∆M).

Mass accuracy: Expressed in parts per million, this value indicates how much an instrument deviates from a known standard or calibrant.

Scan rate: Measured in hertz, the scan rate of an MS indicates how quickly it scans a mass spectrum.

Polarity switch: The collection of both positive and negative sample ionization data (spectra) by the ion trap during a single MS analysis. Polarity switching is applied to the study of drugs, drug metabolites, pesticides, etc.

PRM: Parallel reaction monitoring (PRM) is a targeted method of high resolution MS quantification in which a full scan of each precursor ion transition is monitored; i.e., all fragments derived from the precursor ion are monitored. In contrast, single reaction monitoring (SRM) performs one transition monitoring at a time.

Multiplex: The ability of a mass analyzer to acquire data from multiple precursor ions in the same scan without compromising high signal-to-noise ratio.

MSn: Also termed tandem MS, the use of multiple stage MS to capture product ion spectra of a defined m/z, where n is the number of recorded product ion stages.

ETD: Electron transfer dissociation (ETD) results in the fragmentation of the peptide backbone of MS samples, simplifying identification of peptide modification sites.

Synchronous MS3: A method of quantitation that involves simultaneous selection of several MS2 precursors for MS3 analysis and is often used during TMT and iTRAQ experiments.


Tomorrow's Quantitation Compendium

Tomorrow's Quantitation Compendium

Developing robust quantitation assays is possible with both triple quadrupole and Orbitrap-based high-resolution MS instruments. Both technologies enable confident quantitation in a variety of sample types. Access a comprehensive list of methods and publications for multiple sample types using both technologies.

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LC-MS webinar series

LC-MS webinar series

Expand your application knowledge

Our LC-MS on-demand webinar series expands your application knowledge for pharma, biopharma, environmental, omics, food, forensics and clinical research and more. Learn how the latest high-performance mass spectrometry solutions can enable your laboratory to solve your most pressing analytical challenges, regardless of sample type.