Choose from an extensive portfolio of high-resolution accurate-mass (HRAM) Thermo Scientific Orbitrap mass analyzers. Our Orbitrap-based MS systems connect to liquid chromatography equipment to enhance separation of unknown compounds and enable high-throughput workflows. Some of our Orbitrap mass analyzers combine with ion traps and quadrupole mass filters. Compare products below to find the Orbitrap LC-MS instruments that meet your analysis needs the best.
Choose the high-resolution accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your lab
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| Q Exactive Focus |
Q Exactive |
Q Exactive Plus |
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|---|---|---|---|---|
| Ideal Applications | Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Targeted Metabolomics | Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Targeted Metabolomics | Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Sport Anti-Doping, Biopharma development, Metabolomics, Lipidomics | |
| Analyses | MS and MS/MS, Qual/Quan, Screening & Confirmation | |||
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |||
| Sample thoughtput | Low to medium | Medium to high | Medium to high |
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| Standard Features | ||||
| Resolving Power @ m/z 200 | 70,000 | 140,000 | 140,000 (up to 280,000 with BioPharma option) |
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| Mass Accuracy, (Internal Callibration) | <1 ppm with internal calibration |
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| Mass Range | m/z 50 - 3,000 | m/z 50 - 6,000 (up to 8000 with option) |
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| Scan Speed | Up to 12 Hz | |||
| Dynamic Range | Up to 5 orders | |||
| Ion Source | IonMax with H-ESI II probe | |||
| Polarity Switching | 1 full cycle <1 sec at 35,000 resolution setting | |||
| Multiplexing | 3 (SIM only) | 10 | ||
| Scan Functions | SIM | SIM & PRM | ||
| Dissociation | In-source CID, HCD | |||
| Optional Upgrades | ||||
| Intact Protein Mode | N/A | Yes | ||
| Enhanced Resolution Mode | N/A | Yes | ||
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For more information
Choose the high performance, high-resolution accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your translational research lab
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| Orbitrap Exploris 480 | Q Exactive HF-X |
Q Exactive HF |
Q Exactive UHMR |
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|---|---|---|---|---|---|---|---|
| Ideal Applications | Proteomics, BioPharma | Proteomics, BioPharma, Metabolomics | Proteomics, Structural Biology | ||||
| Analyses | MS and MS/MS, Qual/Quan, Screening & Confirmation | MS and MS/MS, Screening & Confirmation | |||||
| Advanced Data-Dependent Experiments | SureQuant, Precursor Fit, Boxcar Scanning | N/A | |||||
| Almanac Web-Based Monitoring and Management | Require Foundation 3.1 SP1 or later | ||||||
| Standard Features | |||||||
| Resolving Power | 480,000 at m/z 195 | 240,000 at m/z 200 | 120,000 at m/z 200 (up to 240,000 with option) |
200,000 at m/z 400 | |||
| Mass Accuracy (Internal Callibration) |
<1 ppm internal calibration | ||||||
| Mass Range | m/z 40 - 6000 (up to 8000 with BioPharma option) |
m/z 50 - 6,000 (up to 8000 with BioPharma option) |
m/z 350 - 80,000 | ||||
| Scan Speed | Up to 18 Hz | Up to 40 Hz | Up to 18 Hz | Up to 12 Hz (at resolution setting of 12,500 @ m/z 400 | |||
| Ion Source | (1 full cycle <1 sec) | ||||||
| Polarity Switching | OptaMax NG | IonMax with H-ESI II probe | |||||
| Multiplexing | Up to 20 precursors/scan | N/A | |||||
| Scan Functions | FS, AIF, SIM, t-MS2, DIA, ddHCD | FS, AIF, SIM, PRM, DIA, ddHCD | FS, AIF, MS/MS, pseudo-MS3 | ||||
| Dissociation | In-source CID, IRM | In-source CID, HCD | In-source trapping, HCD | ||||
| Optional Upgrades | |||||||
| Intact Protein Mode | Yes | ||||||
| High Mass Rangen(HMRn, up to 8000 m/z) | Yes | N/A | |||||
| Easy-IC Ion Source | Yes | N/A | |||||
| FAIMS Pro Interface | Yes | N/A | |||||
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High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
For more information
Choose the maximum performance Tribrid Orbitrap mass spectrometer that's right for your discovery lab
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| Orbitrap ID-X Tribrid MS | Orbitrap Fusion Lumos Tribrid MS | Orbitrap Eclipse Tribrid MS | ||||||
|---|---|---|---|---|---|---|---|---|
| Ideal Applications | Small molecule characterization and elucidation research for applications such as Metabolomics, Drug development Met ID, Extactables and Leachables, Industrial Environmental Health and more | Multi-omics, TMT, Intact/top-down proteomics, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking | Multi-omics, TMT, Intact/top-down proteomics, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking, NativeOmics, LC-top-down mass spectrometry, Biotherapeutics | |||||
| Analyses | MS and MSn, Qualitative and Quantitative, Profiling, screening, and targeted consistently cap or uncap | |||||||
| Advanced Data-Dependent Experiments | Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, MSn Library builder | SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quanfirmation Acquisition, SureQuant | RTS SPS MS3 ,Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quanfirmation Acquisition, SureQuant, Precursor FitFilter | |||||
| AcquireX Acquisition Tool | Requires Thermo Scientific Orbitrap Tribrid Series 4.2 SP1QF1, 3.1 or greater | |||||||
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |||||||
| Standard Features | ||||||||
| Resolution (FWHM) @m/z 200 | 7,500-500,000 | 7,500-500,000 (1M optional) |
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| Mass Accuracy (internal callibration) | <3 ppm (<1ppm) | |||||||
| Mass Range | m/z 50 - 2,000 | m/z 50 - 6,000 | m/z 50 - 6,000 (up to 8000 with HMRn) |
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| Ion Source | OptaMax NG | |||||||
| Ion Optics | S-lens | High-Capacity Ion Transfer Tube (HCTT), Electrodynamic Ion Funnel (EDIF) | ||||||
| Scan Speed (Under Defined Conditions) | 30 Hz (OT) 40 Hz (IT) | 40 Hz (OT) 45 Hz (IT) | ||||||
| Polarity Switching | One full cycle in 1.1 sec (positive and negative full scan with resolution of 30,000) | |||||||
| Scan Functions | Full scan, MS/MS, MSn, SIM, PRM, DIA, AIF | Full scan, MS/MS, MSn, SIM, PRM, DIA, AIF, TurboTMT | ||||||
| MSn Level | Up to 10 | |||||||
| Minimum Quadrupole Mass Filter | 0.4 amu | |||||||
| Linear Ion Trap Isolation | 0.2 to 600 amu | 0.2 to 4000 amu | ||||||
| Dissociation | In-source CID, HCD | |||||||
| Optional Upgrades | ||||||||
| ETD/EThcD | No | EASY-ETD | ||||||
| UVPD Laser | No | Yes | ||||||
| High Mass Rangen (HMRn, up to 8,000 m/z) | No | Yes | ||||||
| Proton Transfer Charged Reactions (PTCR) | No | Yes | ||||||
| EASY-IC Ion Source | Yes | |||||||
| 1M Resolution | No | Yes | ||||||
| FAIMS Pro Interface | No | Yes | ||||||
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||||||||
High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
For more information
Choose the high-resolution accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your lab
|
|
|
||
| Q Exactive Focus |
Q Exactive |
Q Exactive Plus |
||
|---|---|---|---|---|
| Ideal Applications | Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Targeted Metabolomics | Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Targeted Metabolomics | Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Sport Anti-Doping, Biopharma development, Metabolomics, Lipidomics | |
| Analyses | MS and MS/MS, Qual/Quan, Screening & Confirmation | |||
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |||
| Sample thoughtput | Low to medium | Medium to high | Medium to high |
|
| Standard Features | ||||
| Resolving Power @ m/z 200 | 70,000 | 140,000 | 140,000 (up to 280,000 with BioPharma option) |
|
| Mass Accuracy, (Internal Callibration) | <1 ppm with internal calibration |
|||
| Mass Range | m/z 50 - 3,000 | m/z 50 - 6,000 (up to 8000 with option) |
||
| Scan Speed | Up to 12 Hz | |||
| Dynamic Range | Up to 5 orders | |||
| Ion Source | IonMax with H-ESI II probe | |||
| Polarity Switching | 1 full cycle <1 sec at 35,000 resolution setting | |||
| Multiplexing | 3 (SIM only) | 10 | ||
| Scan Functions | SIM | SIM & PRM | ||
| Dissociation | In-source CID, HCD | |||
| Optional Upgrades | ||||
| Intact Protein Mode | N/A | Yes | ||
| Enhanced Resolution Mode | N/A | Yes | ||
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For more information
Choose the high performance, high-resolution accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your translational research lab
|
|
|
|
||||
| Orbitrap Exploris 480 | Q Exactive HF-X |
Q Exactive HF |
Q Exactive UHMR |
||||
|---|---|---|---|---|---|---|---|
| Ideal Applications | Proteomics, BioPharma | Proteomics, BioPharma, Metabolomics | Proteomics, Structural Biology | ||||
| Analyses | MS and MS/MS, Qual/Quan, Screening & Confirmation | MS and MS/MS, Screening & Confirmation | |||||
| Advanced Data-Dependent Experiments | SureQuant, Precursor Fit, Boxcar Scanning | N/A | |||||
| Almanac Web-Based Monitoring and Management | Require Foundation 3.1 SP1 or later | ||||||
| Standard Features | |||||||
| Resolving Power | 480,000 at m/z 195 | 240,000 at m/z 200 | 120,000 at m/z 200 (up to 240,000 with option) |
200,000 at m/z 400 | |||
| Mass Accuracy (Internal Callibration) |
<1 ppm internal calibration | ||||||
| Mass Range | m/z 40 - 6000 (up to 8000 with BioPharma option) |
m/z 50 - 6,000 (up to 8000 with BioPharma option) |
m/z 350 - 80,000 | ||||
| Scan Speed | Up to 18 Hz | Up to 40 Hz | Up to 18 Hz | Up to 12 Hz (at resolution setting of 12,500 @ m/z 400 | |||
| Ion Source | (1 full cycle <1 sec) | ||||||
| Polarity Switching | OptaMax NG | IonMax with H-ESI II probe | |||||
| Multiplexing | Up to 20 precursors/scan | N/A | |||||
| Scan Functions | FS, AIF, SIM, t-MS2, DIA, ddHCD | FS, AIF, SIM, PRM, DIA, ddHCD | FS, AIF, MS/MS, pseudo-MS3 | ||||
| Dissociation | In-source CID, IRM | In-source CID, HCD | In-source trapping, HCD | ||||
| Optional Upgrades | |||||||
| Intact Protein Mode | Yes | ||||||
| High Mass Rangen(HMRn, up to 8000 m/z) | Yes | N/A | |||||
| Easy-IC Ion Source | Yes | N/A | |||||
| FAIMS Pro Interface | Yes | N/A | |||||
|
|||||||
High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
For more information
Choose the maximum performance Tribrid Orbitrap mass spectrometer that's right for your discovery lab
|
|
|
||||||
| Orbitrap ID-X Tribrid MS | Orbitrap Fusion Lumos Tribrid MS | Orbitrap Eclipse Tribrid MS | ||||||
|---|---|---|---|---|---|---|---|---|
| Ideal Applications | Small molecule characterization and elucidation research for applications such as Metabolomics, Drug development Met ID, Extactables and Leachables, Industrial Environmental Health and more | Multi-omics, TMT, Intact/top-down proteomics, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking | Multi-omics, TMT, Intact/top-down proteomics, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking, NativeOmics, LC-top-down mass spectrometry, Biotherapeutics | |||||
| Analyses | MS and MSn, Qualitative and Quantitative, Profiling, screening, and targeted consistently cap or uncap | |||||||
| Advanced Data-Dependent Experiments | Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, MSn Library builder | SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quanfirmation Acquisition, SureQuant | RTS SPS MS3 ,Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quanfirmation Acquisition, SureQuant, Precursor FitFilter | |||||
| AcquireX Acquisition Tool | Requires Thermo Scientific Orbitrap Tribrid Series 4.2 SP1QF1, 3.1 or greater | |||||||
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |||||||
| Standard Features | ||||||||
| Resolution (FWHM) @m/z 200 | 7,500-500,000 | 7,500-500,000 (1M optional) |
||||||
| Mass Accuracy (internal callibration) | <3 ppm (<1ppm) | |||||||
| Mass Range | m/z 50 - 2,000 | m/z 50 - 6,000 | m/z 50 - 6,000 (up to 8000 with HMRn) |
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| Ion Source | OptaMax NG | |||||||
| Ion Optics | S-lens | High-Capacity Ion Transfer Tube (HCTT), Electrodynamic Ion Funnel (EDIF) | ||||||
| Scan Speed (Under Defined Conditions) | 30 Hz (OT) 40 Hz (IT) | 40 Hz (OT) 45 Hz (IT) | ||||||
| Polarity Switching | One full cycle in 1.1 sec (positive and negative full scan with resolution of 30,000) | |||||||
| Scan Functions | Full scan, MS/MS, MSn, SIM, PRM, DIA, AIF | Full scan, MS/MS, MSn, SIM, PRM, DIA, AIF, TurboTMT | ||||||
| MSn Level | Up to 10 | |||||||
| Minimum Quadrupole Mass Filter | 0.4 amu | |||||||
| Linear Ion Trap Isolation | 0.2 to 600 amu | 0.2 to 4000 amu | ||||||
| Dissociation | In-source CID, HCD | |||||||
| Optional Upgrades | ||||||||
| ETD/EThcD | No | EASY-ETD | ||||||
| UVPD Laser | No | Yes | ||||||
| High Mass Rangen (HMRn, up to 8,000 m/z) | No | Yes | ||||||
| Proton Transfer Charged Reactions (PTCR) | No | Yes | ||||||
| EASY-IC Ion Source | Yes | |||||||
| 1M Resolution | No | Yes | ||||||
| FAIMS Pro Interface | No | Yes | ||||||
|
||||||||
High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
For more information
Definition of terms
Resolution: Frequently reported in full width at half maximum (FWHM), resolution is the separation of two mass spectrometry peaks and is calculated as the mass of a peak (M) divided by resolving power (∆M).
Mass accuracy: Expressed in parts per million, this value indicates how much an instrument deviates from a known standard or calibrant.
Scan rate: Measured in hertz, the scan rate of an MS indicates how quickly it scans a mass spectrum.
Polarity switch: The collection of both positive and negative sample ionization data (spectra) by the ion trap during a single MS analysis. Polarity switching is applied to the study of drugs, drug metabolites, pesticides, etc.
PRM: Parallel reaction monitoring (PRM) is a targeted method of high resolution MS quantification in which a full scan of each precursor ion transition is monitored; i.e., all fragments derived from the precursor ion are monitored. In contrast, single reaction monitoring (SRM) performs one transition monitoring at a time.
Multiplex: The ability of a mass analyzer to acquire data from multiple precursor ions in the same scan without compromising high signal-to-noise ratio.
MSn: Also termed tandem MS, the use of multiple stage MS to capture product ion spectra of a defined m/z, where n is the number of recorded product ion stages.
ETD: Electron transfer dissociation (ETD) results in the fragmentation of the peptide backbone of MS samples, simplifying identification of peptide modification sites.
Synchronous MS3: A method of quantitation that involves simultaneous selection of several MS2 precursors for MS3 analysis and is often used during TMT and iTRAQ experiments.
In this symposia Prof. Dr. Albert J.R. Heck discusses new approaches for structural proteomics workflows for crosslinking and native MS.
