Screen, Confirm and Quantify – Q Exactive Orbitrap MS

Choose the Q Exactive Orbitrap Hybrid MS that's right for your lab

  Q Exactive Plus
Attributes Q Exactive
Focus
Q Exactive
 
Q Exactive
Plus
Q Exactive
HF
Q Exactive
HF-X
Q Exactive
UHMR
Max resolution (FWHM) @m/z 200 70k 140k 140k
(Opt. 280k)
240k 200k at
m/z 400
Mass accuracy, (internal) <1 ppm
Mass accuracy (external) <3 ppm
Mass range 50 to 3,000 m/z 50 to 6,000 m/z 50 to 8,000 m/z 350 to 80,000 m/z
Dynamic Range >5000:1
Scan rates Up to 12 Hz Up to 18 Hz Up to 40 Hz Up to 12 Hz (at resolution setting of 12,500 @ m/z 400)
Polarity switch <1 sec
PRM - Yes
Multiplex (precursor/ scan) - 10
Performance and Value
Go beyond with Orbitrap Tribrid MS for large and small molecule analysis

Choose the Orbitrap Tribrid MS that's right for your lab

  Orbitrap Fusion
Attributes Orbitrap Fusion Orbitrap Fusion Lumos Orbitrap ID-X
Max resolution (FWHM) @m/z 200 500k 1,000k 7.5K–500k
Mass accuracy, (internal) <1ppm

<1 ppm RMS using internal calibration

<3 ppm RMS using external calibration, 

Mass range - - 50–2,000 m/z
Scan rates 20Hz 40Hz

30 Hz Orbitrap mass analyzer MSn

40 Hz ion trap MS

Polarity switch < 1.1 sec 1.1 sec
PRM Yes -
Multiplex (precursor/ scan) 10 20 10
MSn (n=10) Yes Yes
ETD option Yes -
Decision-tree (CID/HCD/ ETD) Yes -
Synch MS3 Yes -
Performance and Value

For more information

Screen, Confirm and Quantify – Q Exactive Orbitrap MS

Choose the Q Exactive Orbitrap Hybrid MS that's right for your lab

  Q Exactive Plus
Attributes Q Exactive
Focus
Q Exactive
 
Q Exactive
Plus
Q Exactive
HF
Q Exactive
HF-X
Q Exactive
UHMR
Max resolution (FWHM) @m/z 200 70k 140k 140k
(Opt. 280k)
240k 200k at
m/z 400
Mass accuracy, (internal) <1 ppm
Mass accuracy (external) <3 ppm
Mass range 50 to 3,000 m/z 50 to 6,000 m/z 50 to 8,000 m/z 350 to 80,000 m/z
Dynamic Range >5000:1
Scan rates Up to 12 Hz Up to 18 Hz Up to 40 Hz Up to 12 Hz (at resolution setting of 12,500 @ m/z 400)
Polarity switch <1 sec
PRM - Yes
Multiplex (precursor/ scan) - 10
Performance and Value
Go beyond with Orbitrap Tribrid MS for large and small molecule analysis

Choose the Orbitrap Tribrid MS that's right for your lab

  Orbitrap Fusion
Attributes Orbitrap Fusion Orbitrap Fusion Lumos Orbitrap ID-X
Max resolution (FWHM) @m/z 200 500k 1,000k 7.5K–500k
Mass accuracy, (internal) <1ppm

<1 ppm RMS using internal calibration

<3 ppm RMS using external calibration, 

Mass range - - 50–2,000 m/z
Scan rates 20Hz 40Hz

30 Hz Orbitrap mass analyzer MSn

40 Hz ion trap MS

Polarity switch < 1.1 sec 1.1 sec
PRM Yes -
Multiplex (precursor/ scan) 10 20 10
MSn (n=10) Yes Yes
ETD option Yes -
Decision-tree (CID/HCD/ ETD) Yes -
Synch MS3 Yes -
Performance and Value

For more information


Definition of terms

Resolution: Frequently reported in full width at half maximum (FWHM), resolution is the separation of two mass spectrometry peaks and is calculated as the mass of a peak (M) divided by resolving power (∆M).

Mass accuracy: Expressed in parts per million, this value indicates how much an instrument deviates from a known standard or calibrant.

Scan rate: Measured in hertz, the scan rate of an MS indicates how quickly it scans a mass spectrum.

Polarity switch: The collection of both positive and negative sample ionization data (spectra) by the ion trap during a single MS analysis. Polarity switching is applied to the study of drugs, drug metabolites, pesticides, etc.

PRM: Parallel reaction monitoring (PRM) is a targeted method of high resolution MS quantification in which a full scan of each precursor ion transition is monitored; i.e., all fragments derived from the precursor ion are monitored. In contrast, single reaction monitoring (SRM) performs one transition monitoring at a time.

Multiplex: The ability of a mass analyzer to acquire data from multiple precursor ions in the same scan without compromising high signal-to-noise ratio.

MSn: Also termed tandem MS, the use of multiple stage MS to capture product ion spectra of a defined m/z, where n is the number of recorded product ion stages.

ETD: Electron transfer dissociation (ETD) results in the fragmentation of the peptide backbone of MS samples, simplifying identification of peptide modification sites.

Synchronous MS3: A method of quantitation that involves simultaneous selection of several MS2 precursors for MS3 analysis and is often used during TMT and iTRAQ experiments.


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