Choose from an extensive portfolio of Thermo Scientific high-resolution, accurate-mass (HRAM) Orbitrap mass analyzers. Our Orbitrap-based MS systems connect to liquid chromatography instruments to enhance separation of unknown and known compounds and enable high-throughput workflows. Compare products below to find the Thermo Scientific Orbitrap LC-MS instruments that best meet your analysis needs.
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| Q Exactive Plus MS |
Orbitrap Exploris 120 MS |
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|---|---|---|
| Ideal Applications | Forensic Toxicology, Clinical Research, Food & Environmental Safety Research, Basic Academic Research, Sport Anti-Doping, Biopharma development, Metabolomics, Lipidomics | Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Targeted/semi-targeted Metabolomics, Pharmaceuticals |
| Analyses | Unknown identification & confirmation, non-targeted and targeted screening and targeted quantitation | |
| Next-generation ion source interface | N/A | Yes |
| Quick start methods for various applications | N/A | Yes |
| Drag-n-drop method setup user interface | N/A | Yes |
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |
| AcquireX Intelligent data acquisition | N/A | |
| Standard Features | ||
| Resolving Power @ m/z 200 | 140,000 (up to 280,000 with BioPharma option) |
120,000 |
| Mass Accuracy (Internal Calibration) |
<1 ppm with internal calibration | <1 ppm for at least 5 days with EASY-IC ion source |
| Calibration procedures | Traditional Calmix | One-click calibration with FlexMix calibration solution |
| Mass Range | m/z 50 - 6,000 (up to 8000 with BioPharma option) |
m/z 40 - 3,000 |
| Scan Speed | 12 Hz | 22 Hz |
| Dynamic Range | Up to 5 orders | |
| Ion Source interface | IonMax with H-ESI II probe | OptaMax NG ion source |
| Polarity Switching | 1 Hz | 1.4 Hz |
| Multiplexing | 10 | 20 |
| Scan Functions | Full-MS, AIF, SIM, PRM, (t-MS2 for Orbitrap Exploris 120), DIA | |
| Dissociation | In-source CID, HCD | |
| Optional Upgrades | ||
| Intact Protein Mode | Yes | N/A |
| Enhanced Resolution Mode | Yes | N/A |
| Sources | APCI/APPI Combination Ion Source EASY-Spray Sources Nanospray Flex Ion Sources |
APCI/APPI Combination Ion Source EASY-Spray Sources Nanospray Flex Ion Sources |
For more information
Choose the high performance, high-resolution, accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your translational research lab
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| Orbitrap Exploris 240 | Orbitrap Exploris 480 | Q Exactive UHMR |
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|---|---|---|---|---|
| Ideal Applications | Forensic Toxicology, Sport Anti-Doping, Food & Environmental Safety research and emerging contaminant testing, Extractables & Leachables, Academic Research, Biopharma development, Semi-targeted Metabolomics, Lipidomics, Clinical & Translational Research | Quantitative Proteomics, Protein Identification, Biopharma R&D, Clinical & Translational Research | Proteomics, Structural Biology, Protein Characterization | |
| Analyses | MS and MS/MS, Qual/Quan, Screening & Confirmation | MS and MS/MS, Screening & Confirmation | ||
| Advanced Data-Dependent Experiments | Precursor Fit | SureQuant, Precursor Fit, Boxcar Scanning | N/A | |
| Next-generation ion source interface | Yes | N/A | ||
| Quick start methods for various applications | Yes | N/A | ||
| Drag-and-drop method setup user interface | Yes | N/A | ||
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |||
| AcquireX intelligent data acquisition | Yes | N/A | ||
| Standard Features | ||||
| Resolving Power @ m/z 200 | 240,000 | 480,000 | 200,000 at m/z 400 | |
| Mass Accuracy (Internal Calibration) |
<1 ppm for up to 5 days with EASY-IC ion source | <1 ppm internal calibration | ||
| Calibration procedures | One-click calibration with FlexMix calibration solution | Traditional Calmix | ||
| EASY-IC Ion Source | <1 ppm for up to 5 days | N/A | ||
| Dynamic Range | Up to 5 orders | |||
| Mass Range | m/z 40 - 6000 (up to 8000 with BioPharma option) |
m/z 40 - 6000 (up to 8000 with BioPharma option) |
m/z 350 - 80,000 | |
| Scan Speed | Up to 22 Hz | Up to 40 Hz | Up to 12 Hz (at resolution setting of 12,500 @ m/z 400 | |
| Polarity Switching | 1.4 Hz | (1 full cycle <1 sec) | ||
| Ion Source | OptaMax NG ion source | IonMax with H-ESI II probe | ||
| Multiplexing | Up to 20 precursors/scan | N/A | ||
| Scan Functions | FS, AIF, SIM, t-MS2, DIA, ddHCD | FS, AIF, MS/MS, pseudo-MS3 | ||
| Dissociation | In-source CID, HCD | In-source trapping, HCD | ||
| Optional Upgrades | ||||
| Intact Protein Mode | Yes | |||
| High Mass Rangen(HMRn, up to 8000 m/z) | Yes | N/A | ||
| Sources | FAIMS Pro Interface APCI/APPI Combination Ion Source EASY-Spray Sources Nanospray Flex Ion Sources VeriSpray PaperSpray Ion Source |
APCI/APPI Combination Ion Source EASY-Spray Sources Nanospray Flex Ion Sources |
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High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
For more information
Choose the maximum performance Tribrid Orbitrap mass spectrometer that's right for your discovery lab
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| Orbitrap ID-X Tribrid MS | Orbitrap Fusion Lumos Tribrid MS | Orbitrap Eclipse Tribrid MS | ||||||
|---|---|---|---|---|---|---|---|---|
| Ideal Applications | Small molecule characterization and elucidation research for applications such as Metabolomics, Drug development Met ID, Extactables and Leachables, Industrial Environmental Health and more | Multi-omics, TMT, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking | Multi-omics, TMT, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking, NativeOmics, LC-top-down mass spectrometry, Biotherapeutics | |||||
| Analyses | MS and MSn, Qualitative and Quantitative, Profiling, screening, and targeted consistently cap or uncap | |||||||
| Advanced Data-Dependent Experiments | Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, MSn Library builder | SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quantitation/Confirmation Acquisition, SureQuant | Real-Time Search SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quantitation/Confirmation Acquisition, SureQuant, Precursor Fit Filter | |||||
| AcquireX Acquisition Tool | Requires Thermo Scientific Orbitrap Tribrid Series 4.2 SP1QF1, 3.1 or greater | |||||||
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |||||||
| Optional Upgrades | ||||||||
| EASY-ETD HD | No | Yes | ||||||
| UVPD Laser | No | Yes | ||||||
| High Mass Rangen (HMRn, up to 8,000 m/z) | No | Yes | ||||||
| Proton Transfer Charge Reduction (PTCR) | No | Yes | ||||||
| EASY-IC Ion Source | Yes | |||||||
| 1M Resolution | No | Yes | ||||||
| FAIMS Pro Interface | Yes | |||||||
 |
||||||||
High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS technology successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
|
|
|
| Q Exactive Plus MS |
Orbitrap Exploris 120 MS |
|
|---|---|---|
| Ideal Applications | Forensic Toxicology, Clinical Research, Food & Environmental Safety Research, Basic Academic Research, Sport Anti-Doping, Biopharma development, Metabolomics, Lipidomics | Forensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Targeted/semi-targeted Metabolomics, Pharmaceuticals |
| Analyses | Unknown identification & confirmation, non-targeted and targeted screening and targeted quantitation | |
| Next-generation ion source interface | N/A | Yes |
| Quick start methods for various applications | N/A | Yes |
| Drag-n-drop method setup user interface | N/A | Yes |
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |
| AcquireX Intelligent data acquisition | N/A | |
| Standard Features | ||
| Resolving Power @ m/z 200 | 140,000 (up to 280,000 with BioPharma option) |
120,000 |
| Mass Accuracy (Internal Calibration) |
<1 ppm with internal calibration | <1 ppm for at least 5 days with EASY-IC ion source |
| Calibration procedures | Traditional Calmix | One-click calibration with FlexMix calibration solution |
| Mass Range | m/z 50 - 6,000 (up to 8000 with BioPharma option) |
m/z 40 - 3,000 |
| Scan Speed | 12 Hz | 22 Hz |
| Dynamic Range | Up to 5 orders | |
| Ion Source interface | IonMax with H-ESI II probe | OptaMax NG ion source |
| Polarity Switching | 1 Hz | 1.4 Hz |
| Multiplexing | 10 | 20 |
| Scan Functions | Full-MS, AIF, SIM, PRM, (t-MS2 for Orbitrap Exploris 120), DIA | |
| Dissociation | In-source CID, HCD | |
| Optional Upgrades | ||
| Intact Protein Mode | Yes | N/A |
| Enhanced Resolution Mode | Yes | N/A |
| Sources | APCI/APPI Combination Ion Source EASY-Spray Sources Nanospray Flex Ion Sources |
APCI/APPI Combination Ion Source EASY-Spray Sources Nanospray Flex Ion Sources |
For more information
Choose the high performance, high-resolution, accurate-mass (HRAM) Orbitrap mass spectrometer that's right for your translational research lab
|
|
|
||
| Orbitrap Exploris 240 | Orbitrap Exploris 480 | Q Exactive UHMR |
||
|---|---|---|---|---|
| Ideal Applications | Forensic Toxicology, Sport Anti-Doping, Food & Environmental Safety research and emerging contaminant testing, Extractables & Leachables, Academic Research, Biopharma development, Semi-targeted Metabolomics, Lipidomics, Clinical & Translational Research | Quantitative Proteomics, Protein Identification, Biopharma R&D, Clinical & Translational Research | Proteomics, Structural Biology, Protein Characterization | |
| Analyses | MS and MS/MS, Qual/Quan, Screening & Confirmation | MS and MS/MS, Screening & Confirmation | ||
| Advanced Data-Dependent Experiments | Precursor Fit | SureQuant, Precursor Fit, Boxcar Scanning | N/A | |
| Next-generation ion source interface | Yes | N/A | ||
| Quick start methods for various applications | Yes | N/A | ||
| Drag-and-drop method setup user interface | Yes | N/A | ||
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |||
| AcquireX intelligent data acquisition | Yes | N/A | ||
| Standard Features | ||||
| Resolving Power @ m/z 200 | 240,000 | 480,000 | 200,000 at m/z 400 | |
| Mass Accuracy (Internal Calibration) |
<1 ppm for up to 5 days with EASY-IC ion source | <1 ppm internal calibration | ||
| Calibration procedures | One-click calibration with FlexMix calibration solution | Traditional Calmix | ||
| EASY-IC Ion Source | <1 ppm for up to 5 days | N/A | ||
| Dynamic Range | Up to 5 orders | |||
| Mass Range | m/z 40 - 6000 (up to 8000 with BioPharma option) |
m/z 40 - 6000 (up to 8000 with BioPharma option) |
m/z 350 - 80,000 | |
| Scan Speed | Up to 22 Hz | Up to 40 Hz | Up to 12 Hz (at resolution setting of 12,500 @ m/z 400 | |
| Polarity Switching | 1.4 Hz | (1 full cycle <1 sec) | ||
| Ion Source | OptaMax NG ion source | IonMax with H-ESI II probe | ||
| Multiplexing | Up to 20 precursors/scan | N/A | ||
| Scan Functions | FS, AIF, SIM, t-MS2, DIA, ddHCD | FS, AIF, MS/MS, pseudo-MS3 | ||
| Dissociation | In-source CID, HCD | In-source trapping, HCD | ||
| Optional Upgrades | ||||
| Intact Protein Mode | Yes | |||
| High Mass Rangen(HMRn, up to 8000 m/z) | Yes | N/A | ||
| Sources | FAIMS Pro Interface APCI/APPI Combination Ion Source EASY-Spray Sources Nanospray Flex Ion Sources VeriSpray PaperSpray Ion Source |
APCI/APPI Combination Ion Source EASY-Spray Sources Nanospray Flex Ion Sources |
||
High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
For more information
Choose the maximum performance Tribrid Orbitrap mass spectrometer that's right for your discovery lab
|
|
|
||||||
| Orbitrap ID-X Tribrid MS | Orbitrap Fusion Lumos Tribrid MS | Orbitrap Eclipse Tribrid MS | ||||||
|---|---|---|---|---|---|---|---|---|
| Ideal Applications | Small molecule characterization and elucidation research for applications such as Metabolomics, Drug development Met ID, Extactables and Leachables, Industrial Environmental Health and more | Multi-omics, TMT, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking | Multi-omics, TMT, Intact/top-down proteomics, Targeted Quantification, Protein-Protein Crosslinking, NativeOmics, LC-top-down mass spectrometry, Biotherapeutics | |||||
| Analyses | MS and MSn, Qualitative and Quantitative, Profiling, screening, and targeted consistently cap or uncap | |||||||
| Advanced Data-Dependent Experiments | Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, MSn Library builder | SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quantitation/Confirmation Acquisition, SureQuant | Real-Time Search SPS MS3, Universal Method, Product Ion & Neutral Loss Triggered-MSn, Isolation offset, Quantitation/Confirmation Acquisition, SureQuant, Precursor Fit Filter | |||||
| AcquireX Acquisition Tool | Requires Thermo Scientific Orbitrap Tribrid Series 4.2 SP1QF1, 3.1 or greater | |||||||
| Almanac Web-Based Monitoring and Management | Requires Foundation 3.1 SP1 or later | |||||||
| Optional Upgrades | ||||||||
| EASY-ETD HD | No | Yes | ||||||
| UVPD Laser | No | Yes | ||||||
| High Mass Rangen (HMRn, up to 8,000 m/z) | No | Yes | ||||||
| Proton Transfer Charge Reduction (PTCR) | No | Yes | ||||||
| EASY-IC Ion Source | Yes | |||||||
| 1M Resolution | No | Yes | ||||||
| FAIMS Pro Interface | Yes | |||||||
|
||||||||
High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS technology successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
Definition of terms
Resolution: Frequently reported in full width at half maximum (FWHM), resolution is the separation of two mass spectrometry peaks and is calculated as the mass of a peak (M) divided by resolving power (∆M).
Mass accuracy: Expressed in parts per million, this value indicates how much an instrument deviates from a known standard or calibrant.
Scan rate: Measured in hertz, the scan rate of an MS indicates how quickly it scans a mass spectrum.
Polarity switch: The collection of both positive and negative sample ionization data (spectra) by the ion trap during a single MS analysis. Polarity switching is applied to the study of drugs, drug metabolites, pesticides, etc.
PRM: Parallel reaction monitoring (PRM) is a targeted method of high resolution MS quantification in which a full scan of each precursor ion transition is monitored; i.e., all fragments derived from the precursor ion are monitored. In contrast, single reaction monitoring (SRM) performs one transition monitoring at a time.
Multiplex: The ability of a mass analyzer to acquire data from multiple precursor ions in the same scan without compromising high signal-to-noise ratio.
MSn: Also termed tandem MS, the use of multiple stage MS to capture product ion spectra of a defined m/z, where n is the number of recorded product ion stages.
ETD: Electron transfer dissociation (ETD) results in the fragmentation of the peptide backbone of MS samples, simplifying identification of peptide modification sites.
Synchronous MS3: A method of quantitation that involves simultaneous selection of several MS2 precursors for MS3 analysis and is often used during TMT and iTRAQ experiments.
Tomorrow's Quantitation Compendium
Developing robust quantitation assays is possible with both triple quadrupole and Orbitrap-based high-resolution MS instruments. Both technologies enable confident quantitation in a variety of sample types. Access a comprehensive list of methods and publications for multiple sample types using both technologies.
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