Screen, Confirm and Quantify – Q Exactive Orbitrap MS

High-resolution accurate-mass LC-MS instruments for emerging needs

Thermo Scientific hybrid quadrupole-Orbitrap mass spectrometers enable both targeted and non-targeted screening and characterization. They also work with a broad range of sample type applications so that your laboratory can go from compound discovery to quantitation without relying on different instruments and technologies. With the combination of scan speed, high resolution, mass accuracy, spectral quality, and sensitivity you can achieve quantifiable certainty.

Contact us   Grant writing support   FDA NDMA method

On this page

Genotoxic impurity analysis for NDMA in drug products using Orbitrap LC-MS

More versatility

Q Exactive Plus Hybrid
Mass Spectrometer

Move from application to application with ease.

Learn more

High-resolution native MS

Q Exactive UHMR Hybrid
Mass Spectrometer

Improve disease understanding and therapeutic effectiveness.

Learn more

Inside the Q Exactive Hybrid Quadrupole-Orbitrap

  1. RF lens: The radio frequency (RF) lens stacked-ring RF ion guide captures and focuses ions into a tight beam after they exit the ionization source, increasing sensitivity. Large variable spacing between electrodes enables better pumping efficiency and improved ruggedness. Bent flatapole ion guide reduces noise by preventing neutrals and high-velocity clusters from entering the quadrupole.
  2. AABG: Reducing noise and extending maintenance intervals, the Advanced Active Beam Guide (AABG) enables intelligent ion beam management for high flux ion sources.
  3. Quadrupole Mass Filter with AQT: The mass filter improves precursor selection and ion transmission for more accurate quantitation of low abundance analytes in complex matrices. It also offers variable precursor ion isolation width selection from 0.4 Da to full mass range.
  4. Orbitrap mass analyzer: The Orbitrap mass analyzer offers unprecedented high resolution accurate mass (HRAM) analysis of ions as a function of their rotational frequencies; these data are converted to mass spectra using fast Fourier transforms (FFTs).
  5. HCD cell: Ions pass from the nitrogen-filled C-trap into this multipole collision cell and undergo fragmentation. Afterwards, HCD voltage is increased and the ions are transferred back to the C-trap, to be injected into the Orbitrap for detection.

Which Q Exactive Orbitrap Mass Spectrometer is best for my lab?

Our hybrid quadrupole-Orbitrap mass spectrometry systems vary in terms of mass resolution, scan speed and analysis modes. Ideal for mass spectral analysis for targeted and high-throughput analysis of low-level components in complex matrices applications in biopharma, forensic toxicology, clinical and translational research, environmental and food testing. To find the system best suited to your laboratory’s specific needs, compare Q Exactive Orbitrap mass spectrometers using data in the chart below.

 Q Exactive PlusQ Exactive UHMR
 Q Exactive
Q Exactive
Ideal ApplicationsForensic Toxicology, Clinical Research, Food & Environmental Safety Testing, Basic Academic Research, Sport Anti-Doping, Biopharma development, Metabolomics, LipidomicsProteomics, Structural Biology
AnalysesMS and MS/MS, Qual/Quan, Screening & ConfirmationMS and MS/MS, Screening & Confirmation
Almanac Web-Based Monitoring and ManagementRequire Foundation 3.1 SP1 or later
Standard Features
Resolving Power140,000
(up to 280,000 with BioPharma option)
200,000 at m/z 400
Mass Accuracy
(Internal Calibration)
<1 ppm with internal calibration
Mass Rangem/z 50 - 6,000
(up to 8000 with option)
m/z 350 - 80,000
Scan SpeedUp to 12 HzUp to 12 Hz (at resolution setting of 12,500 @ m/z 400
Ion SourceIonMax with H-ESI II probe(1 full cycle <1 sec)
Polarity Switching1 full cycle <1 sec at 35,000 resolution settingIonMax with H-ESI II probe
Scan FunctionsSIM & PRMFS, AIF, MS/MS, pseudo-MS3
DissociationIn-source CID, HCDIn-source trapping, HCD
Optional Upgrades
Intact Protein ModeYes
High Mass Rangen(HMRn, up to 8000 m/z)YesN/A
Enhanced Resolution ModeYes
Performance and Value

Keep up with the speed of science the lab-forward trade-in program
Keep up with the speed of science

The Lab-Forward Trade-In Program

The world needs science to move faster. Now more than ever. Which is why when more scientists have access to the latest scientific instrumentation, we all benefit. That is the sole purpose of our Lab-Forward Trade-In Program. For a limited time, trade in your chromatography and mass spectrometry instruments and save!

Learn more

High Resolution Mass Spectrometry with Orbitrap Mass Analyzer for Screening, Confirmative and Quantitative Analyses

Learn how Orbitrap mass analyzer instruments enable consistent analytical certainty even in the most complex matrices based on their high and ultra-high mass resolution capabilities, high sensitivity and selectivity in full scan (and MS/MS) acquisition, mass accuracy and precision (robustness) and ease of use.

Download white paper

Laboratories performing LC-MS applications strive for certainty when identifying detected compounds. For many small molecule applications, conventional approaches aren’t enough. Laboratories require more, such as rich high quality spectral information and fit for purpose data analysis software to make satisfactory identifications and characterization.

Compound Discoverer software helps you extract high-confidence understanding from comprehensive Q Exactive high-resolution accurate-mass data.

Q Exactive Orbitrap MS ordering guide

Style Sheet for Global Design System
CMD SchemaApp code