Intact non-reduced antibody characterization

Get deep structural insight to complex proteins and protein interactions

The complexity of modern therapeutic proteins, such as antibody-drug conjugates (ADCs), presents a significant analytical challenge requiring high resolution chromatography combined with high resolution mass spectrometry. ADCs involving cysteine conjugation present a unique challenge for characterization as proper intact analysis requires native mass spectrometry conditions to preserve structurally-critical non-covalent binding between antibody chains. Intact protein analysis under native mass spectrometric conditions can now be performed routinely using mass spec compatible buffers together with size exclusion (SEC) or ion exchange chromatography (IEC) coupled to Thermo Scientific Orbitrap Exploris BioPharma series mass spectrometers.

Speak to a specialist  Join our community


On this page

Native intact mass analysis workflow


Featured chromatography solutions for native intact mass

Top


Orbitrap mass spectrometers for native intact mass analysis

  Orbitrap Exploris 480 Mass Spectrometer Orbitrap Eclipse Tribrid Mass Spectrometer Q Exactive UHMR Orbitrap
Exploris 240 MS
  Orbitrap Exploris 480 Mass Spectrometer Orbitrap Eclipse Tribrid Mass Spectrometer Orbitrap Fusion Lumos Tribrid Mass Spectrometer Orbitrap Exploris 240
Advantages Extraordinary simplified within a compact footprint where next level exploration delivers richer insights gained with intact protein analysis under native mass spectrometric conditions, empowering all by bringing performance and intelligence together. Go beyond today's discovery with new innovations that deliver the ultimate flexibility to expand experimental scope, with built-in intelligence to ensure the highest data quality and confidence with intact protein analysis under native mass spectrometric conditions. Go beyond what you thought possible with an outstanding tool for investigating the higher order structure, native-like tertiary and quaternary protein structures with a high resolution accurate mass and an ability to analyze ultrahigh mass range. Realize complete BioPharma characterization with distinct operational modes optimized for protein analysis, and take advantage of the extended mass range up to m/z 8000 available with the BioPharma Option for native MS analysis for antibody drug conjugates and other heterogeneous antibody samples.
Resources
Resolving power 480,000 at m/z 195 7,500-500,000 FWHM at m/z 200 (1M option) 200,000 FWHM at m/z 400 (1M option) Up to 240,000 FWHM at m/z 200
Scan speed Up to 40Hz Up to 40Hz 12Hz at resolution setting of 12,500 at m/z 400 Up to 22Hz at resolution setting 15,000 at m/z 200
Mass range 40 to 6,000 m/z (up to 8,000 m/z with the BioPharma option) 50 to 6,000 (up to 8,000 with HMRn) 350 to 80,000 40 to 8,000 m/z
Dynamic range >5000:1 >5000 with a single OTMS spectrum >5,000 with a single OTMS spectrum >5000 within a single Orbitrap mass analyzer spectrum
Mass accuracy Internal <1 ppm RMS; External: <3 ppm RMS Internal <1 ppm RMS; External: <3 ppm RMS Internal <1 ppm RMS; External: <3 ppm RMS Internal <1 ppm RMS; External: <3 ppm RMS

Top


Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

Learn more  Watch free webinar


Featured software for native intact mass analysis


Three ways to spend less and get more

Affordability made easy

Interested in financing options, purchasing a factory-certified refurbished LC-MS instrument or trading-in your existing mass spectrometer for something better? Choose from a wide selection of high performance systems from the most routine, for all levels of experience, to the most sophisticated and advanced research. We can get you to greater productivity at an affordable price.

Learn more

Top


Watch videos on native intact mass analysis


Featured intact protein analysis resource
Intact and Native Characterization webinars
Intact and Native Characterization webinars

Join experts from Genentech and Thermo Fisher to get great structural insight into your drug product using Orbitrap high resolution accurate mass spectrometers.

Resources

Brochures

Case studies

Application notes & posters