Intact non-reduced antibody characterization

Get deep structural insight to complex proteins and protein interactions

The complexity of modern therapeutic proteins, such as antibody-drug conjugates (ADCs), presents a significant analytical challenge requiring high resolution chromatography combined with high resolution mass spectrometry. ADCs involving cysteine conjugation present a unique challenge for characterization as proper intact analysis requires native mass spectrometry conditions to preserve structurally-critical non-covalent binding between antibody chains. Intact protein analysis under native mass spectrometric conditions can now be performed routinely using mass spec friendlily buffers together with size exclusion (SEC) or ion exchange chromatography (IEC) coupled to Q Exactive BioPharma series mass spectrometers.

Featured native intact mass analysis products

Native intact mass analysis workflow

Native Intact Mass Analysis workflow
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Native intact mass analysis categories

State-of-the-art intact protein analysis

The BioPharma Option for the Thermo Scientific Q Exactive Plus and Q Exactive HF Hybrid Quadrupole-Orbitrap mass spectrometers provides a complete BioPharma characterization solution. With the introduction of the high mass range (HMR) mode the platform is optimized for both conventional intact monoclonal antibody analysis performed in denaturing conditions, as well as native MS analysis which is ideal for antibody drug conjugates and other heterogeneous antibody samples.

Go beyond what you thought was possible with Native MS

While analysis of denatured proteins may be the most common LC/MS protein analysis, many biopharmaceutical and biological research applications require the analysis of protein-protein interactions and protein conformations in their native (non-denatured) state. An outstanding tool for investigating the higher order structure, native-like tertiary and quaternary protein structures is the new Thermo Scientific Q Exactive UHMR, which combines unsurpassed high resolution accurate mass Thermo Scientific Orbitrap analysis with an ability to analyze ultrahigh mass range up to 80,000 m/z.

Protein Deconvolution Algorithms
Powerful protein deconvolution algorithms

BioPharma Finder integrated software provides confident protein deconvolution to determine the molecular weight of proteins in both denaturing and native conditions. Protein sequence matching identifies N-linked glycosylation and other common post-translational modifications (PTMs) via intact mass analysis, helping you to fully understand the heterogeneity of your biologic drug.

Three ways to spend less and get more

Three ways to spend less and get more

Upgrade your ability to achieve new scientific discoveries faster with Thermo Scientific Orbitrap mass analyzers that set new standards in sensitivity, performance and productivity. Or take advantage of upgrades to extend your current Thermo Scientific LC-MS system capabilities.

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Featured videos

Featured intact protein analysis resource
Intact and Native Characterization webinars
Intact and Native Characterization webinars

Join experts from Genentech and Thermo Fisher to get great structural insight into your drug product using the Q Exactive BioPharma platform of mass spectrometers.