Intact non-reduced antibody characterization

The complexity of modern therapeutic proteins, such as antibody-drug conjugates (ADCs), presents a significant analytical challenge requiring high resolution chromatography combined with high resolution mass spectrometry. ADCs involving cysteine conjugation present a unique challenge for characterization as proper intact analysis requires native mass spectrometry conditions to preserve structurally-critical non-covalent binding between antibody chains. Intact protein analysis under native mass spectrometric conditions can now be performed routinely using size exclusion chromatography (SEC) coupled to the Thermo Scientific™ Q Exactive™ Plus or the Thermo Scientific™ Q Exactive™ HF mass spectrometers.

Featured native intact mass analysis products

Native intact mass analysis workflow


Native intact mass analysis categories

High-resolution separation of proteins and peptides under non-denaturing conditions

Achieve high-resolution separation of proteins and peptides under non-denaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns. Developed with multifunctional ligand attachments, these silica-based columns provide exceptional hydrolytic stability under the highly aqueous conditions used in hydrophobic interaction chromatography (HIC).

Superior separations under non-denaturing conditions

Use Thermo Scientific™ MAbPac™ SEC-1 Size Exclusion Columns for high-resolution separation of monoclonal antibody (mAb) analysis, including monomers and aggregates. These size-exclusion chromatography (SEC) columns provide superior performance, under non-denaturing conditions, and in high- and low-salt mobile phases and volatile eluents.

Separation of native monoclonal antibodies by hydrophobic interaction chromatography

Designed for separation of mAbs and related biologics by hydrophobic interaction (HIC), the Thermo Scientific™ MAbPac™ HIC-10 LC column is a high-resolution silica column with a unique chemistry that provides excellent biocompatibility. Its selectivity is different from other HIC columns on the market.

Chromatography built for biopharma

Use the innovative architecture of a UHPLC system that has been tailored to the specific and demanding analytical requirements that these essential drugs impose. The Thermo Scientific™ Vanquish™ Flex Binary and Quaternary systems have been designed and manufactured to exacting engineering standards to be truly built for biopharma.

Unify workflows for chromatography and routine quantitative MS analysis. Thermo Scientific™ Chromeleon™ 7.2 Chromatography Data System (CDS) software provides full integration of Thermo Scientific™ gas chromatography (GC)-MS/MS and liquid chromatography (LC)-MS/MS instruments. Quickly and easily process and report UV and MS data in one application. Run your analyses in an enterprise environment—from method creation to quantitation and library-based compound identification.

State-of-the-art intact protein analysis

The BioPharma Option for the Thermo Scientific™ Q Exactive™ Plus and Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometers provides a complete BioPharma characterization solution. With the introduction of the high mass range (HMR) mode the platform is optimized for both conventional intact monoclonal antibody analysis performed in denaturing conditions, as well as native MS analysis which is ideal for antibody drug conjugates and other heterogeneous antibody samples.

Investigate the structure, topology, and architecture of native-like tertiary and quaternary protein structures

While analysis of denatured proteins may be the most common LC/MS protein analysis, many biopharmaceutical and biological research applications require the analysis of protein-protein interactions and protein conformations in their native (non-denatured) state. An outstanding tool for investigating the higher order structure, topology, and architecture of native-like tertiary and quaternary protein structures is the Thermo Scientific™ Exactive™ Plus EMR mass spectrometer, which combines unsurpassed high resolution accurate mass Thermo Scientific™ Orbitrap™ analysis with an extended mass range (EMR) option.

Powerful protein deconvolution algorithms

Thermo Scientific™ BioPharma Finder™ integrated software provides confident protein deconvolution to determine the molecular weight of proteins in both denaturing and native conditions. Protein sequence matching identifies N-linked glycosylation and other common post-translational modifications (PTMs) via intact mass analysis, helping you to fully understand the heterogeneity of your biologic drug.