mAb characterization, aggregate, fragment and titer analyses

Characterization of mAbs and their aggregates, fragments, and titers

Extensive development of protein and monoclonal antibody (mAb) therapeutics currently underway requires innovative and efficient tools that characterize and quantify structural variants and modifications, monitor product stability and demonstrate safety and efficacy. These specifically designed Thermo Scientific MAbPac columns provide a full range of high resolution, high efficiency mAb analyses by LC and LC-MS.

Monoclonal antibody LC columns
Reversed phase monoclonal antibody columns
Reversed phase monoclonal antibody columns

Now you can use reversed-phase conditions for higher resolution separation of intact mAbs and fragments by HPLC and LC-MS.

Hydrophobic interaction chromatography (HIC) mAb columns

Use these columns for mAb, mAb fragment, antibody-drug aggregate, and related biologic separations.

Hydrophobic interaction chromatography (HIC) mAb columns
Affinity monoclonal antibody columns
Affinity monoclonal antibody columns

Use these columns for fast and accurate titer analysis of monoclonal antibodies in harvest cell cultures.

Strong cation exchange monoclonal antibody columns

Use these columns for high-resolution, high-efficiency analysis of mAbs and associated variants.

Strong cation exchange monoclonal antibody columns
Size exclusion mAb columns
Size exclusion monoclonal antibody columns

Choose these columns for optimal mAb analysis, including the high-resolution separation of monomers, aggregates and fragments.

Which column to choose for monoclonal antibody analysis?

Analysis

Description

Columns & Buffers

mAb Capture & titer analysis
  • mAb titer determination (concentration) & screening
  • mAb capture for analysis workflows
Aggregate analysis Routing screening for mAb aggregates and fragments
Charge variant analysis Routine charge variant profiling/screening, including lysine truncation, deamidation & acylation
Methionine & tryptophan oxidation Targeted analysis of methionine and tryptophan oxidation
Antibody drug conjugate (ADC) analysis ADC DAR analysis
Intact mAb & fragment analysis
  • Light chain (LC) and heavy chain (HC) analysis
  • Fab and Fc analysis
  • scFc and F(ab’)2 analysis
Sequence & structural analysis
  • Primary sequence analysis
  • peptide mapping
  • peptide & glycopeptides structural & linkage analysis
Glycan profiling Profiling of released glycans
Featured monoclonal antibody analysis videos

This interactive tool will guide you through the products we offer for antibody analysis and purification. Start by selecting an area of interest to display relevant information. Continue making selections that interest you.
 

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Featured LC / LC-MS webinar
 Fast, robust LC and LCMS workflows for the comprehensive characterization of biotherapeutic proteins
Fast, robust LC and LC-MS workflows for the comprehensive characterization of biotherapeutic proteins

Over the past decade, there have been a growing number of mAb candidates entering the clinical pipeline. This results in a large increase on the demand for analytical characterization.

Register to watch webinar ›