Protein Aggregate Analysis

Rapidly determine protein aggregation

Aggregation of therapeutic proteins can result in an incorrect drug dosage, or and undesired and potentially fatal immune response. Consequently, monitoring protein aggregates is important for safety and quality assurance.

Investigators routinely use size-exclusion chromatography (SEC), which separates particles according to hydrodynamic size. Recently the use of hydrophobic interaction chromatography (HIC) has also received great interest as an orthogonal technique to SEC.

HIC separates proteins based on the hydrophobicity in the native state and can often detect changes in protein structure as well as aggregates.

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Protein aggregation assay workflow

Protein aggregation assay workflow

Protein aggregation analysis categories
SEC for protein aggregation detection

Use Thermo Scientific MAbPac SEC-1 Size Exclusion Columns for high-resolution separation of monoclonal antibody (mAb) analysis, including the monomers, aggregates and Fab and Fc fragments resulting from proteolysis. These size exclusion chromatography (SEC) columns provide superior performance, even under non-denaturing conditions, and in high- and low-salt mobile phases and volatile eluents. 

Chromatography system built for biopharma characterization

Obtain new benchmarks in accuracy, precision, and sensitivity in a biocompatible UHPLC system with state-of-the-art quaternary solvent blending. The Thermo Scientific Vanquish Flex Quaternary UHPLC system shares all the values inherent to the Vanquish product line, including an innovative design, focus on uptime, robustness, and reliability. Multiple detection options as well as Thermo Scientific Viper Fingertight Fitting connections throughout the system give you the performance you need with the ease of use you want. The combination makes this system ideal for biopharmaceutical and pharmaceutical applications, including the separation of aggregates and other higher order structures.

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BioPharma Charge Variant & Aggregate Analysis eBook

As biopharmaceutical development expands, so too does the need for analytical techniques that address key challenges of throughput and robustness. Get this new eBook and learn how to overcome these challenges with novel ion exchange (IEC) and size exclusion (SEC) chromatography workflows.

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