Invitrogen Alexa Fluor Plus secondary antibodies

Make your low-abundance targets visible, spend less time optimizing, and make every one of your precious samples count. Invitrogen Alexa Fluor Plus conjugate technology provides higher signal to noise ratios with minimized cross-reactivity for fluorescent imaging and western blotting.

See Alexa Fluor Plus antibodies

Alexa Fluor Plus 405

Alexa Fluor Plus 405 conjugates allow for improved signal to noise when compared to traditional Alexa Fluor 405 conjugates. Traditional Alexa Fluor 405 secondary antibody (left) compared to Alexa Fluor Plus 405 secondary antibody (right, goat anti-rabbit IgG secondary antibody, (Cat No. A48254) in immunofluorescent analysis of β-III tubulin in fixed HeLa cells. Nuclei were stained with SYTOX Deep Red stain (Cat. No S11380). The images contain overlay of tubulin (cyan), and nuclei (magenta).  Images were taken on an EVOS M7000 Imaging system (Cat. No. AMF7000) at 20X magnification.

Product list: Alexa Fluor Plus 405 Secondary Antibodies
Goat anti-rabbitA48254
Goat anti-mouseA48255
Goat anti-chickenA48260
Goat anti-ratA48261
Goat anti-humanA48275
Donkey anti-goatA48259
Donkey anti-rabbitA48258
Donkey anti-mouseA48257
Donkey anti-ratA48268
Alexa Fluor Plus 488

Alexa Fluor Plus 488 allows you to see greater detail compared to traditional Alexa Fluor 488 conjugates. Traditional Alexa Fluor 488 secondary antibody (left) compared to Alexa Fluor Plus 488 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat. No. A32723) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of neuronal tubulin (green) and nuclei (blue). Images were taken on a confocal microscope at 40x magnification.

Product list: Alexa Fluor Plus 488 Secondary Antibodies*
Goat anti-rabbitA32731
Goat anti-mouseA32723
Goat anti-chickenA32931
Goat anti-ratA48262
Goat anti-humanA48276
F(ab’)2-goat anti-rabbitA48282
F(ab’)2-goat anti-mouseA48286
Donkey anti-goatA32814
Donkey anti-rabbitA32790
Donkey anti-mouseA32766
Donkey anti-ratA48269

*All Alexa Fluor Plus 488 conjugates available in small pack sizes

Alexa Fluor Plus 555

Alexa Fluor Plus 555 allows you to see greater detail compared to traditional Alexa Fluor 555 conjugates. Traditional Alexa Fluor 555 secondary antibody (left) compared to Alexa Fluor Plus 555 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat. No. A32727) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of neuronal tubulin (orange) and nuclei (blue). Images were taken on a confocal microscope at 40x magnification.

Product list: Alexa Fluor Plus 555 Secondary Antibodies
Goat anti-rabbitA32732
Goat anti-mouseA32727
Goat anti-chickenA32932
Goat anti-ratA48263
Goat anti-humanA48277
F(ab’)2-goat anti-rabbitA48283
F(ab’)2-goat anti-mouseA48287
Donkey anti-goatA32816
Donkey anti-rabbitA32794
Donkey anti-mouseA32773
Donkey anti-ratA48270
Alexa Fluor Plus 594

Alexa Fluor Plus 594 allows you to see greater detail compared to traditional Alexa Fluor 594 conjugates. Traditional Alexa Fluor 594 secondary antibody (left) compared to Alexa Fluor Plus 594 secondary antibody (right, donkey anti-mouse IgG secondary antibody (Cat. No. A32744). Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570) in immunofluorescent analysis of tubulin in U2OS cells. The image contains overlay of tubulin (red) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System with an Olympus 20X Super Apochromat objective (Cat. No. AMEP4734) at 20X magnification.

Product list: Alexa Fluor Plus 594 Secondary Antibodies
Goat anti-rabbitA32740
Goat anti-mouseA32742
Goat anti-chickenA32759
Donkey anti-goatA32758
Donkey anti-rabbitA32754
Donkey anti-mouseA32744
Alexa Fluor Plus 647

Alexa Fluor Plus 647 allows you to see greater detail compared to traditional Alexa Fluor 647 conjugates. Traditional Alexa Fluor 647 secondary antibody (left) compared to Alexa Fluor Plus 647 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat. No. A32728) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of neuronal tubulin (pink) and nuclei (blue). Images were taken on a confocal microscope at 40x magnification.

Product list: Alexa Fluor Plus 647 Secondary Antibodies*
Goat anti-rabbitA32733
Goat anti-mouseA32728
Goat anti-chickenA32933
Goat anti-ratA48265
Goat anti-humanA48279
F(ab’)2-goat anti-rabbitA48285
F(ab’)2-goat anti-mouseA48289
Donkey anti-goatA32849
Donkey anti-rabbitA32795
Donkey anti-mouseA32787
Donkey anti-ratA48272

*All Alexa Fluor Plus 647 conjugates available in small pack sizes

Alexa Fluor Plus 680

Alexa Fluor Plus 680 allows you to see greater detail compared to traditional Alexa Fluor 680 conjugates. Traditional Alexa Fluor 680 secondary antibody (left) compared to Alexa Fluor Plus 680 secondary antibody (right, goat anti-chicken IgY secondary antibody, (Cat. No. A32934) in Immunofluorescent analysis of tubulin in A549 cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of tubulin (purple) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System with an Olympus 20X Super Apochromat objective (Cat. No. AMEP4734) at 20X magnification.

Product list: Alexa Fluor Plus 680 Secondary Antibodies
Goat anti-rabbitA32734
Goat anti-mouseA32729
Goat anti-chickenA32934
Donkey anti-goatA32860
Donkey anti-rabbitA32802
Donkey anti-mouseA32788
Alexa Fluor Plus 800

Alexa Fluor Plus 800 shows more bands at lower dilutions, providing greater sensitivity and range of detection compared to traditional 800 near-IR conjugates. Competitor, 800 near-IR secondary antibody (bottom) compared to Alexa Fluor Plus 800 secondary antibody (top, goat anti-rabbit IgG secondary antibody, Cat. No. A32735) in a western blot analysis of heat shock protein 90 (Hsp90) and histone deacetylase 1 (HDAC1).

Product list: Alexa Fluor Plus 800 Secondary Antibodies
Goat anti-rabbitA32735
Goat anti-mouseA32730
Goat anti-chickenA32935
Donkey anti-goatA32930
Donkey anti-rabbitA32808
Donkey anti-mouseA32789


Invitrogen Alexa Fluor Plus Secondary Antibody products

 Alexa Fluor Plus 405Alexa Fluor Plus 488*Alexa Fluor Plus 555Alexa Fluor Plus 594Alexa Fluor Plus 647*Alexa Fluor Plus 680Alexa Fluor Plus 800
Goat anti-rabbitA48254A32731A32732A32740A32733A32734A32735
Goat anti-mouseA48255A32723A32727A32742A32728A32729A32730
Goat anti-chickenA48260A32931A32932A32759A32933A32934A32935
Goat anti-ratA48261A48262A48263A48264A48265——
Goat anti-humanA48275A48276A48277A48278A48279——
Donkey anti-goatA48259A32814A32816A32758A32849A32860A32930
Donkey anti-rabbitA48258A32790A32794A32754A32795A32802A32808
Donkey anti-mouseA48257A32766A32773A32744A32787A32788A32789
Donkey anti-ratA48268A48269A48270A48271A48272——

*All Alexa Fluor Plus 488 and 647 conjugates available in small pack sizes


Invitrogen Alexa Fluor Plus F(ab’)2 Secondary Antibodies

Our new Alexa Fluor Plus conjugated F(ab’)2 secondary antibodies are cross-adsorbed or pre-adsorbed to increase sensitivity and reduce background staining. F(ab')2 fragment antibodies are divalent antibodies, containing two Fab portions, that are generated by pepsin digestion from a whole IgG antibody in order to remove the Fc region. F(ab’)2 antibodies are approximately 110kDa and because of their smaller size they can more easily penetrate complex tissue models than whole IgG molecules, potentially leading to more antigen binding capability. This property combined with Alexa Fluor Plus conjugation makes these molecules valuable staining tools in immunohistochemistry and immunofluorescent experiments.

 Alexa Fluor Plus 488*Alexa Fluor Plus 555Alexa Fluor Plus 594Alexa Fluor Plus 647*
F(ab’)2-goat anti-rabbitA48282A48283A48284A48285
F(ab’)2-goat anti-mouseA48286A48287A48288A48289

*All Alexa Fluor Plus 488 and 647 F(ab’)2 conjugates available in small pack sizes.

A

Comparison of whole IgG with F(ab’)2 Alexa Fluor Plus 555 secondary antibody in human small intestine (Duodenum)

B

Comparison of whole IgG with F(ab’)2 Alexa Fluor Plus 555 secondary antibody in human small intestine (Duodenum)

Comparison of whole IgG with F(ab’)2 Alexa Fluor Plus 555 secondary antibody in human small intestine (Duodenum). (A) Cell junctions were stained using Invitrogen Cadherin-17 Monoclonal Antibody (Cat No. 60351-1-IG) and Invitrogen Alexa Fluor Plus 555 whole IgG secondary antibody. (B) Under matched conditions, cell junctions were stained using the same primary antibody against Cadherin 17 (Cat. No. 60351-1-IG) and Invitrogen F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (Cat. No. A48287). Nuclei were stained with Hoechst 33342 (Cat. No. H3570). Images were captured on an Invitrogen EVOS M7000 at 4x magnification.

See real scientists’ reactions to the Alexa Fluor Plus images

Alexa Fluor Plus secondary antibody information & conjugates

Use
  • Designed to help detect and visualize low-abundance targets in rare or precious samples
Applications
Product attributes
  • Higher signal-to-noise ratio than traditional Alexa Fluor secondary antibodies
    • Up to 4.2 times higher signal to noise in immunofluorescence (IF)/ Immunocytochemistry (ICC) imaging
    • Up to 5.8 times higher signal to noise in fluorescent western blotting (WB)
  • Lower cross-reactivity compared to traditional Alexa Fluor secondary antibodies

Fluorophores

Targets

  • Goat anti-rabbit
  • Goat anti-mouse
  • Goat anti-chicken
  • Goat anti-rat
  • Goat anti-human
  • Donkey anti-goat
  • Donkey anti-rabbit
  • Donkey anti-mouse
  • Donkey anti-rat  
Mountant compatibility
  • Most standard mountants

Recommended:

Fixative compatibility

  • Most standard fixatives
Recommended:
Organelle staining compatibility
  • Most standard organelle-specific antibodies and stains
Recommended:

*All Alexa Fluor Plus 488 and 647 conjugates available in small pack sizes

Alexa Fluor Plus new packaging

New packaging helps save time and space

  • Storage made easier—the new vials have flagless labels. No more struggling to wrap the flag around the vial to fit it into racks or centrifuges.
  • Identifying made easier—vials have colored caps corresponding to the Alexa Fluor Plus dye of the secondary antibody conjugate. If you are looking for an Alexa Fluor Plus 488 antibody, just look for the green cap.
  • Easier to see how much antibody you have left—vials are clear so you can easily see the volume remaining. No need to pipette from an opaque or solid-colored vial just to estimate how much antibody remains.