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Custom Affinity Ligand and Resin Development
CaptureSelect purification products enable the efficient capture of therapeutic proteins from any feed stream, thereby helping reduce the number of process steps, increase yield, as well as provide a platform approach for purifying any target biotherapeutic.
We offer a unique service for the development of product- and/or process-specific affinity solutions tailored to a target protein and its specific requirements.
The custom ligand can be immobilized on a variety of backbones, including POROS resins, and further developed into an affinity resin, which can be used in large-scale processing of biopharmaceuticals.
Custom CaptureSelect resin development program
| Library construction 1 to 10 targets | Lead screening 200 → 24 → 6 | Prototype resin testing 6 → 2 | Lead selection 2 → 1 | cGMP suitable resin |
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Ligand screen:
| Prototype screen:
| Lead testing:
| Final resin:
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Request a technical meeting
No matter what your development challenges are, we are here to help. Our experienced team of scientists and technical specialists is ready to assist you with custom application solution. Complete this form to start the conversation and move towards a custom solution designed just for you.
Start from existing candidates in our pipeline
Through our library development process we have identified a number of candidates that may serve as excellent starting points for a custom affinity resin program. Pipeline opportunities include:
Table 1. CaptureSelect affinity products for purification
Product Stage | Therapeutic Proteins | Viruses | Antibody Types |
Research scale purification resins | Antithrombin III (ATIII) | Adenovirus (Adv5) | IgA (Fc) |
Lead Selection | Prothrombin (Factor II) | Influenza (HA) | IgG-Fc (mouse) |
Prototype resin testing | Universal affinity tag (Uni-Tag) | Baculovirus | |
Lead Screening | Interferon alpha and beta (IFNalpha/beta) | Exosomes | scFv / human VH |
Library Construction | CHO host cell proteins |
| IgG-Fc (rat) |
Technical resources and literature
Posters:
- Poster: Enabling custom solutions for downstream processing
- Poster: C-tag affinity tag, from routine protein purification to use in a cGMP production process
Scientific publications:
- Pabst, TM et al. (2016) Camelid VHH affinity ligands enable separation of closely related biopharmaceuticals. Biotechnol. J. doi: 10.1002/biot.201600357
- Beyer T, Lohse S, Berger S et al.(2009) Serum-free production and purification of chimeric IgA antibodies. J Immunol Methods. 346(1-2):26-37.
- McCue JT, Selvitelli K, Walker J. (2009) Application of a novel affinity adsorbent for the capture and purification of recombinant factor VIII compound. J Chromatogr A. 1216(45):7824-30.
- Kuroiwa Y, Kasinathan P, Sathiyaseelan T et al. (2009) Antigen-specific human polyclonal antibodies from hyperimmunized cattle. Nat Biotechnol. 27(2):173-81
Bioprocessing resources
Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing:
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