Researchers have a wide variety of gene delivery techniques to introduce plasmid DNA, siRNA or duplex RNAi, oligonucleotides, and RNA into eukaryotic cells for a variety of research and drug discovery applications. A review of these techniques is provided on the table below.
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Lipid delivery using cationic lipids
(commercially available) are diluted in separate tubes.
Positive charge on cationic lipid binds to phosphate backbone on nucleic acid.
and clone characterization.
Delivery by electroporation
Pulse cells electrically in the presence of specialized buffer and DNA.
temporary pores in the cell membrane for DNA entry
Delivery by other chemical methods, i. e. calcium phosphate precipitation, DEAE-dextran, polybrene
Allow precipitation of extremely small, insoluble particles containing condensed DNA
Complexes adhere to cell membranes, and enter into the cytoplasm by endocytosis.
Complexes are delivered by osmotic shock using DMSO or glycerol.
Positive-charged polymers complex with negatively charged DNA molecules to enable binding to the cell surface.
Assay for gene expression or silencing.
For Research Use Only. Not for use in diagnostic procedures.