Quick and efficient PCR cloning of highly accurate DNA fragments

Amplification with a high-fidelity proofreading polymerase, such as Invitrogen™ Platinum™ SuperFi™ DNA Polymerase, leaves PCR products with blunt ends rather than 3′-A overhangs, due to the extensive 3′ to 5′ exonuclease activity. Therefore, the vectors supplied in the Invitrogen™ Zero Blunt™ TOPO™ PCR cloning kits contain blunt ends to offer more effective ligation. Choose from over 30 subcloning, sequencing, or long-fragment kits for 95% cloning efficiency in just 5 minutes.

  • Quick—avoid extra ligation steps and laborious searches for appropriate restriction enzymes
  • Efficient—the lethal ccdB gene on our Zero Blunt TOPO vectors acts as a positive-selection method so that only clones with an insert will grow as colonies on your plate
  • Flexible—available with or without competent cells, in multiple reaction sizes and formats to suit your application

Cloning Support Center

Find tips, troubleshooting help, and resources for your cloning applications

Cloning Education

Learn about PCR cloning strategies and subcloning approaches

PCR Education

Review the principles of PCR, components, cycling parameters, methods, and applications

For Research Use Only. Not for use in diagnostic procedures.