Zero Blunt TOPO technology provides a highly efficient, 5-minute, one-step cloning strategy for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases (such as Platinum Superfi) into a plasmid vector.
Each kit uses the pCR 4 Blunt-TOPO vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice.
Each Zero Blunt TOPO PCR Cloning Kit for Sequencing contains linearized and topoisomerase I-activated pCR 4 Blunt-TOPO vector, Salt Solution, dNTPs, Control Template and Primers, M13 Forward and Reverse Primers, One Shot Chemically Competent or Electrocomp E. coli, S.O.C. Medium, and a supercoiled control plasmid.
|Includes One Shot competent E. coli||Advantage||Reactions||Cat. No.|
|TOP10 (genotype)||10||K2875J10||Order now|
|TOP10 (genotype)||25||K287520||Order now |
|TOP10 Electrocomp (genotype)||25||K288020||Order now |
|MAX Efficiency DH5α T1 Phage (genotype)||25||K289520||Order now|
|Mach1 (genotype)||25||K283520||Order now|
|TOP10 (genotype)||50||K287540||Order now|
|TOP10 Electrocomp (genotype)||50||K288040||Order now|
For Research Use Only. Not for use in diagnostic procedures.