Nuclease contamination in reagents used for nucleic acid isolation and analysis can contribute to experimental inconsistency and sometimes even experimental failure. In trying to pinpoint the sources of contamination, it is easy to overlook the water used either to prepare reagents or to resuspend precipitated RNA. Even purified water can have a high pH and minerals that can interfere with reactions that require specific salt and pH conditions. We offer several brands and grades of nuclease-free water—diethylpyrocarbonate (DEPC)-treated water, nuclease-free water (not DEPC-treated), and RT-PCR–grade water—that all have been rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Function Product Description
Nuclease-Free Water Invitrogen Nuclease-Free Water
  • Not DEPC-treated
  • Autoclaved, membrane-filtered
  • RNase and DNase-free
  • Ideal for applications that may be acutely sensitive to residual DEPC (i.e., oocyte injection)
Invitrogen UltraPure DNase⁄RNase-Free Distilled Water
  • Membrane filtered
  • Protease-, RNase-, and DNase-free
  • Designed for use in all molecular applications

DEPC-Treated Water

Invitrogen DEPC-Treated Water
  • Autoclaved, membrane-filtered
  • RNase and DNase-free
  • Autoclaved before and after packaging
Invitrogen UltraPure DEPC-Treated Water
  • Prepared by incubating with 0.1% diethylpyrocarbonate (DEPC)
  • Autoclaved to remove the DEPC; sterile filtered
  • RNase and DNase-free
  • Autoclaved before and after packaging

RT-PCR Grade Water

Invitrogen RT-PCR Grade Water
  • PCR-grade water
  • Each lot tested for nuclease contamination
  • Autoclaved, membrane-filtered
  • RNase-, and DNase-free, genomic DNA-free
  • Ideal for use in any PCR or RT-PCR application

To meet the special challenges of working with RNA, our Invitrogen waters are certified nuclease-free. They are Type II 18-megohm filtered (via state-of-the-art reverse osmosis filtration and deionization), followed by autoclaving and sterile filtration. The purification equipment used to produce Invitrogen waters is subjected to routine maintenance and testing procedures according to rigid ISO 9001 specifications.

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