Nuclease-Free Buffers & Reagents
Once you’ve gone to the trouble of extracting and purifying nucleic acids from your experimental samples, the last thing you want to do is resuspend them in a buffer that may contain nucleases or introduce nucleases with components of downstream reactions. That’s why we offer Ambion® RNA storage buffers and other buffers and reagents that are rigorously tested for nuclease contamination as part of the routine quality control process. These reagents have all been shown to lack detectable nonspecific endonuclease, exonuclease, and RNase activities and meet other product-specific quality requirements.
RNA Storage and Resuspension Buffers
The last step in many nucleic acid isolation protocols, and the first step of many downstream reactions is to resuspend the RNA pellets. After painstakingly isolating RNA, it is crucial to suspend and store your RNA in an appropriate, nuclease-free solution. We offer several Ambion® RNA storage solutions for this purpose. These solutions are prepared from the purest reagents available, under scrupulously monitored conditions in our state-of-the-art manufacturing facility. They are then subjected to rigorous quality control procedures to help ensure their purity and performance.
Buffers and Chemicals
We offer a selection of Ambion® buffers and reagents that are commonly used in reactions containing RNA and DNA. Use these products in your experiments with confidence, knowing that they have passed rigorous quality checks for contaminating nonspecific endonuclease, exonuclease, and RNase activities.
To meet the special challenges of working with RNA, our Ambion® brand waters are certified nuclease-free. They are Type II 18-megohm filtered (via state-of-the-art reverse osmosis filtration and deionization), followed by autoclaving and sterile filtration. The purification equipment used to produce Ambion® waters is subjected to routine maintenance and testing procedures according to rigid ISO 9001 specifications.