ISE-600148160-230x195

Perform your ion concentration measurements rapidly and accurately

Ion concentration measurement or ion-specific (ISE) measurements can be performed in every laboratory for a variety of sample types including water, food and beverage, pharmaceuticals, and biological samples.

To evaluate a sample's ion-specific performance, the best resource is slope S, also known from pH measurement. Whether you're looking to test ISE measurements through the direct method, dilution method, matrix match method, known addition method, or matrix factor method, our robust portfolio of ion-specific meters, electrodes, standards, and solutions are known for delivering accurate and reproducible ISE measurement results.

It begins with your sample

The ion selective electrode (ISE) is a useful and versatile sensor for testing ions in various types of samples, such as environmental, agricultural, industrial, biotechnical, pharmaceutical, food, wastewater, drinking water, and others. Typical ions of interest include ammonia, fluoride, nitrate, chloride, calcium, sodium, potassium, silver, sulfide, iodide, bromide, lead, cadmium, copper, and others.

How does the sample type affect the ISE method that I use for testing?

  • Dilute aqueous (water) samples: Typical dilute aqueous (water) samples can be tested by simple, direct testing. Direct testing involves adding ionic strength adjuster (ISA) solution to standards and samples, calibrating with the standards, and testing the samples.

  • Complex or high ionic strength samples: Some sample types are not dilute aqueous solutions. Samples that are complex or have a high ionic strength background may require a different approach than the direct test method to obtain the best results. In general, high ionic strength samples are samples of >0.1M concentration of dissolved ions or samples that may have a conductivity reading of greater than about 10 mS/cm.

Examples of dilute aqueous samples

          
  • Ultra-pure water
  • Potable water, ground water
  • Surface (natural) water
  • Treated wastewater, effluent
  • Cooling waters, boiler waters
  • Dilute solutions (e.g., < 1000 ppm)

Examples of complex or high ionic strength samples

          
  • Seawater
  • Salt solutions, brines (e.g., >0.5%)
  • Many foods and beverages
  • Acid bath, alkaline solution (e.g, >0.3%)
  • Soil extracts (>0.1M extract solution) buffer solutions

To make an accurate measurement using an ISE, a suitable ISE method should be selected according to specific sample conditions.

  • For complex or high ionic strength samples, addition of ISA may not be sufficient to normalize the ion's activity between the samples and the standards. In that case, choosing an alternate ISE method is recommended for accurate measurements.
     

What if my sample is a solid or is non-aqueous (not water-based)?

  • A solid sample must be prepared so that the ion of interest is dissolved or extracted into water. For example, a certain type of powder, salt, or tablet sample might be mixed with water to dissolve the ion of interest prior to ISE testing. A soil sample may be dried, crushed, and/or sieved, then extracted into a water or salt solution prior to ISE testing. The extracting solution is chosen to bring the ions of interest into solution, and the extracting solution must not interfere with the ISE test.
  • A non-aqueous liquid sample must be prepared so that the ion of interest is extracted into water. For example, an oil sample might be mixed with water or other extracting solution to extract the ion of interest into the water phase. The water phase is then tested. Alternately, the non-aqueous sample might be ashed or subjected to an oxygen combustion bomb preparation (e.g., EPA SW-846 Test Method 5050) to remove the organic matrix. After combustion, the inorganic residue is mixed with water and tested for ions. In some cases, the solid or viscous sample can be added directly to a standard solution and can be tested by analyte addition or subtraction.

Meeting regulatory and compliance standards

ISE-regulated-environment-lg

Regulations vary between ion specific measurements, and it’s critical that you have instruments and electrodes that allow you to stay complaint with your industry standards. Below is a list of international standards for regulatory compliance requirements for a variety of industries.

Regulatory Requirements

International standards for regulatory compliance requirements: 

Parameter

EPA

SM

ASTM

ISO

China MEP

USP

EP

AOAC

Other

pH

EPA 150.14500-H+(B)D1293 (A,B)10523GB/T 6920<791>2.2.3973.41FDA - 21 CFR 114.90

pH of Soil/Solid Waste

SW-846 9040C & 9045D D497210390GB/T15555  994.16 - 994.18USDA NRCS

Conductivity

EPA 120.1; SW-846 9050A2510 (B)D1125 (A)7888n/a<645>2.2.38973.40PSS-78 (salinity)

Dissolved Oxygen

 4500-O (G)D888 (B, C)17289; 5814HJ506    

BOD

 5210 (B) 5815HJ505  973.44 

Fluoride

SW-846 92144500-F (C )D1179 (B)10359GB7484    

Ammonia

 4500-NH3 (D, E) D1426 (B)6778     

Nitrate

Orion 601; SW-846 9210A4500-NO3 (D)       

Sulfide

SW-846 92154500-S2 (G)D4658      

Cyanide

SW-846 92134500-CN (F)D2036 (A)      

Chloride

SW-846 92124500-Cl (D)D512 (C) GB11896    

Bromide

SW-846 9211        
 SM: Standard Methods for the Examination of Water and Wastewater. APHA, AWWA, WEF.
 ASTM: American Society for the Testing and Materials
 ISO: International Organization for Standardization
 USP: United States Pharmacopeia
 EP: European Pharmacopeia
 China MEP: China Ministry of Environmental Protection
 AOAC: Association of Official Analytical Chemists
 NRCS: Natural Resources Conservation Service
 PSS-78: Practical Salinity Scale of 1978
 FDA: U.S. Food and Drug Administration
 USDA: United Stated Department of Agriculture

ISE measurement for a variety of applications

Meet your sample analysis challenges with a combination of Thermo Scientific meters, electrodes, standards, and solutions, ideal for use with the following applications. Click on an icon below to learn more about these products.

ISE Products

 
The accuracy and reliability of your ISE measurements depend on the instrumentation you use. Learn more about how you can identify the best match based on your required features, performance, specifications, and budget

We offer electrodes, standards and electrode storage solutions, electrode cleaning solutions, and electrode filling solutions for your ISE measurement needs, Find te solutions that you need for your process.
 
 
Our advanced analytical technologies and support for your water quality outcomes and compliance no matter how challenging the application.

 

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Available ISE measurement products & solutions

Ion Meters

Perform your routine pH and ion concentration (ISE) measurements in the lab or take your measurements outdoors without sacrificing accuracy and reliability. Thermo Scientific Orion Star A320 series of portable pH and ISE meters offer a rugged, waterproof design for dependable performance on location.

Ion Electrodes

Rely on Orion ion selective electrodes for superior performance and reproducible results. Obtain accuracy by leveraging NIST-traceable calibration standards along with ionic strength adjusters, reagents, and fill solutions for all ISE measurement needs.

Ion Meters

 

Premium

Advanced

Basic

 Orion Versa Star Pro pH/ISE
(VSTAR40 series)
Orion Dual Star pH/ISE Dual Channel
(2115000 series)
Orion Star A214 pH/ISE
(STARA2140 series)
 Premium benchtop Ion Specific (ISE) MetersAdvanced benchtop Ion Specific (ISE) MetersBasic benchtop Ion Specific (ISE) Meters
 Versatile, high-performance laboratory measurement systemEasy to use, dependable laboratory metersEconomical, simplified
laboratory meters
Ideal for
applications in:
Pharmaceuticals, biotechnology, food and beverage, industrialWater, 
wastewater,
chemical, contract testing
Academic, 
environmental, 
power
pH range–2.000 to 20.000 –2.000 to 19.999–2.000 to 20.000 
pH resolution0.001/0.01/0.1 0.001/0.01/0.1 0.001/0.01/0.1 
pH calibration pointsUp to 6 Up to 6Up to 5
mV, RmV modesYes Yes Yes 
ISE Ion Concentration Range0.0001 to 199900.0001 to 199900.0001 to 19990
Incremental techniquesSingle known addition or subtraction, double known addition or subtraction, analyte (sample) addition or subtractionSingle known addition or subtraction, double known addition or subtraction--- 
Temperature Range-5 to 105°C
22 to 221°F
-5 to 105°C
23 to 221°F
-5 to 105°C
23 to 221°F
Number of channels 4, customizable with modules21
Stirrer probe inputs211
Data log2,000 with date/time1,000 with date/time2,000 with date/time
Calibration log30 per mode1010
Identification optionsElectrode ID, sample ID, user IDElectrode ID, sample IDSample ID, user ID
Data export optionsUSB (2), RS232USB, RS232USB, RS232
Power supplyUniversal, 100–240VAC, 50–60Hz (Cat. No. STARA-PWR)Universal, 100–240VAC, 50–60Hz (Cat. No. 1010003)Universal, 100–240VAC, 50–60Hz (Cat. No. 1010003) or 4 AA batteries 
  Enhance your productivity with accessories for your benchtop and portable meters. Meter Accessories ›
 

Premium

Advanced

 Orion Star A329 pH/ISE
(STARA3290 series)
Orion Star A324 pH/ISE
(STARA3240 series)
 Premium portable Ion specific ISE metersAdvanced portable Ion specific ISE meters
 Versatile, powerful, multiparameter meterRugged and dependable multi-parameter meter
Ideal for
applications in:
Industrial—waste water, drinking water, food & beverage; Environmental—lakes, pondsIndustrial—waste water, cooling tanks; Environmental—lakes, ponds
pH range–2.000 to 20.000–2.000 to 20.000
pH resolution0.1/0.01/0.0010.1/0.01/0.001
pH relative accuracy±0.002±0.002
pH calibration pointsUp to 5Up to 5
Range (mV)±2000.0 mV±2000.0 mV
Resolution (mV)0.1 mV0.1 mV
Relative accuracy±0.2 mV or ±0.05% of reading, whichever is greater±0.2 mV or ±0.05% of reading, whichever is greater
EH ORP modeYesYes
Range (ISE)0.0001 to 19,9000.0001 to 19,900
Calibration pointsUp to 5Up to 5
Range (temperature)–5 to 105ºC, 23 to 221ºF–5 to 105ºC, 23 to 221ºF
Number of datalogs5,000 with time and date stamp5,000 with time and date stamp
Data export optionsRS232, USBRS232, USB
AC adapterOptional—universal, 100–240VACOptional—universal, 100-240VAC
Battery powerIncluded—4AAsIncluded—4 AAs
Battery life800 hrs800 hrs
  Enhance your productivity with accessories for your benchtop and portable meters. Meter Accessories ›
 

Premium

Advanced

Basic

 Orion Versa Star Pro pH/ISE
(VSTAR40 series)
Orion Dual Star pH/ISE Dual Channel
(2115000 series)
Orion Star A214 pH/ISE
(STARA2140 series)
 Premium benchtop Ion Specific (ISE) MetersAdvanced benchtop Ion Specific (ISE) MetersBasic benchtop Ion Specific (ISE) Meters
 Versatile, high-performance laboratory measurement systemEasy to use, dependable laboratory metersEconomical, simplified
laboratory meters
Ideal for
applications in:
Pharmaceuticals, biotechnology, food and beverage, industrialWater, 
wastewater,
chemical, contract testing
Academic, 
environmental, 
power
pH range–2.000 to 20.000 –2.000 to 19.999–2.000 to 20.000 
pH resolution0.001/0.01/0.1 0.001/0.01/0.1 0.001/0.01/0.1 
pH calibration pointsUp to 6 Up to 6Up to 5
mV, RmV modesYes Yes Yes 
ISE Ion Concentration Range0.0001 to 199900.0001 to 199900.0001 to 19990
Incremental techniquesSingle known addition or subtraction, double known addition or subtraction, analyte (sample) addition or subtractionSingle known addition or subtraction, double known addition or subtraction--- 
Temperature Range-5 to 105°C
22 to 221°F
-5 to 105°C
23 to 221°F
-5 to 105°C
23 to 221°F
Number of channels 4, customizable with modules21
Stirrer probe inputs211
Data log2,000 with date/time1,000 with date/time2,000 with date/time
Calibration log30 per mode1010
Identification optionsElectrode ID, sample ID, user IDElectrode ID, sample IDSample ID, user ID
Data export optionsUSB (2), RS232USB, RS232USB, RS232
Power supplyUniversal, 100–240VAC, 50–60Hz (Cat. No. STARA-PWR)Universal, 100–240VAC, 50–60Hz (Cat. No. 1010003)Universal, 100–240VAC, 50–60Hz (Cat. No. 1010003) or 4 AA batteries 
  Enhance your productivity with accessories for your benchtop and portable meters. Meter Accessories ›
 

Premium

Advanced

 Orion Star A329 pH/ISE
(STARA3290 series)
Orion Star A324 pH/ISE
(STARA3240 series)
 Premium portable Ion specific ISE metersAdvanced portable Ion specific ISE meters
 Versatile, powerful, multiparameter meterRugged and dependable multi-parameter meter
Ideal for
applications in:
Industrial—waste water, drinking water, food & beverage; Environmental—lakes, pondsIndustrial—waste water, cooling tanks; Environmental—lakes, ponds
pH range–2.000 to 20.000–2.000 to 20.000
pH resolution0.1/0.01/0.0010.1/0.01/0.001
pH relative accuracy±0.002±0.002
pH calibration pointsUp to 5Up to 5
Range (mV)±2000.0 mV±2000.0 mV
Resolution (mV)0.1 mV0.1 mV
Relative accuracy±0.2 mV or ±0.05% of reading, whichever is greater±0.2 mV or ±0.05% of reading, whichever is greater
EH ORP modeYesYes
Range (ISE)0.0001 to 19,9000.0001 to 19,900
Calibration pointsUp to 5Up to 5
Range (temperature)–5 to 105ºC, 23 to 221ºF–5 to 105ºC, 23 to 221ºF
Number of datalogs5,000 with time and date stamp5,000 with time and date stamp
Data export optionsRS232, USBRS232, USB
AC adapterOptional—universal, 100–240VACOptional—universal, 100-240VAC
Battery powerIncluded—4AAsIncluded—4 AAs
Battery life800 hrs800 hrs
  Enhance your productivity with accessories for your benchtop and portable meters. Meter Accessories ›
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