(a) Silencer Select: Life Technologies guarantees that when you purchase two (2) Silencer Select pre-designed siRNA to the same target, then those two siRNAs will silence the target mRNA by 70% or more. To qualify for the guarantee, siRNAs must have been transfected at ≥5 nM and mRNA levels detected 48h post-transfection. Customers must also show ≥70%knockdown with a validated Silencer Select positive control siRNA in the same experiment to demonstrate transfection efficiency. We recommend the use of a Silencer Select non-targeting negative control to normalize mRNA knockdown. If the guaranteed level of knockdown is not observed while an appropriate positive control is successful, a new Silencer Select siRNA will be provided as a free-of-charge replacement. Requests for replacement product must be made within one hundred and eighty (180) days from the date of delivery of the Silencer Select pre-designed siRNAs. This guarantee does not extend to any replacement product nor to siRNAs designed to silence lncRNAs.

(b) Stealth: Life Technologies guarantees that when you purchase three (3) Stealth pre-designed RNAi reagents to the same target, at least two of those reagents will silence the target mRNA by 70% or more. To qualify for the guarantee, Stealth RNAi must have been transfected at ≥20 nM and mRNA levels detected 48h post-transfection. Customers must also show ≥70%knockdown with a Stealth positive control reagent in the same experiment to demonstrate transfection efficiency. We recommend the use of a Stealth negative control to normalize message knockdown. If the guaranteed level of knockdown is not observed while an appropriate positive control is successful, a new Stealth reagent will be provided as a free-of-charge replacement. Requests for replacement product must be made within one hundred and eighty (180) days from the date of delivery of the Stealth pre-designed reagents. This guarantee does not extend to any replacement product.

(c) Silencer: Life Technologies guarantees that when you purchase three (3) Silencer pre-designed siRNAs to the same target, at least two of the siRNAs will silence the target mRNA by 70% or more. To qualify for the guarantee, siRNAs must have been transfected at 100 nM and mRNA levels detected 48h post-transfection. Customers must also show ≥70% knockdown with a validated Silencer positive control siRNA in the same experiment to demonstrate transfection efficiency. We recommend the use of a Silencer non-targeting negative control to normalize mRNA knockdown. If the guaranteed level of knockdown is not observed while an appropriate positive control is successful, a new Silencer siRNA will be provided as a free-of-charge replacement. Requests for replacement product must be made within one hundred and eighty (180) days from the date of delivery of the Silencer pre-designed siRNAs. This guarantee does not extend to any replacement product.

siRNA videos

Meet the inventor: Susan Magdaleno discusses Silencer Select siRNA technology

Dr. Gavin Robertson describes how Silencer Select siRNA technology is advancing melanoma research.

siRNA publications

For Research Use Only. Not for use in diagnostic procedures.