Maximizing transfection efficiency while minimizing cytotoxicity are crucial for optimal gene silencing. Similar to balancing siRNA-induced knockdown and cell viability, there may also be a balance between siRNA delivery and downstream phenotypic assay conditions. It may be necessary to re-optimize siRNA delivery conditions for different downstream assays that are used in siRNA screening passes. The best transfection efficiencies are achieved for each cell type by identifying the following factors (in order of importance):
Once the conditions for maximal gene silencing are determined, keep them constant among experiments with a given cell type.
|High efficiency versatile reagent for a wide range of common cell types||Most efficient versatile reagent for the widest range of cell types including difficult-to-transfect cells||Most efficient reagent for siRNA/miRNA delivery; efficient gene knock-down||High-efficiency electroporation for all cell lines; best for primary and stem cells|
|Lipofectamine 2000||Lipofectamine 3000||Lipofectamine RNAiMAX||Neon Transfection System|
|Top selling format (Cat. No.)||1.5 mL (11668-019)||1.5 mL (L3000-015)||0.75 mL (13778-075)||Starter pack (MPK5000S)|
|Protocol||Download protocol||Download protocol||Download protocol||Download protocol|
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Visit Transfection Basics to learn more about performing transfection in your lab.
For Research Use Only. Not for use in diagnostic procedures.