Product image of Silencer Select Pre-designed siRNAs in multiwell plates

Predesigned Invitrogen Silencer siRNAs are available for all human, mouse, and rat gene targets in the RefSeq database. These siRNAs are designed for maximum potency and specificity using a highly effective and extensively tested algorithm. Each siRNA is synthesized to the highest quality standards and is provided with full sequence information.

When you purchase three predesigned Silencer siRNAs to the same target, we guarantee that at least two of the siRNAs will achieve >70% reduction in target mRNA levels. Visit our siRNA guarantee page for more information.

Order Silencer siRNA positive and negative controls

Optimized design = guaranteed silencing

Silencer Predesigned and Validated siRNAs are designed for maximum potency and specificity using a proprietary algorithm (see: RNAi has become a powerful tool for understanding gene function). The effectiveness of approximately 1,100 algorithm-designed siRNAs was measured in the silencing of almost 400 endogenously expressed human transcripts. This comprehensive survey indicated that, on a per siRNA basis, more than 80% of the individual siRNAs showed >70% silencing of their target. The performance data clearly confirm the success of the Ambion siRNA selection process.

Bar graph showing % target gene knockdown for targets using silencer predesigned siRNAs

Effectiveness of Silencer Predesigned siRNAs. This graph shows the distribution of gene silencing measured for 808 Silencer Predesigned siRNAs targeting >300 endogenously expressed human genes. Target mRNA levels were measured by qRT-PCR 48 hr after transfection into HeLa cells. More than 82% of the siRNAs successfully silenced their targets by >70%.

Validated Silencer siRNAs

A subset of Silencer siRNAs to human targets have been functionally tested in-house and verified to reduce target mRNA levels by 70% or greater, enabling us to guarantee that these Silencer Validated siRNAs will knock-down their target genes. The data sheet provided with each of these siRNAs shows the extent of mRNA knockdown observed during testing and the exon targeted by the siRNA. For most types, full sequence information is also provided at no additional charge.

For  predesigned & validated siRNAs that incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity, please see Silencer Select Predesigned & Validated siRNAs.

We synthesize and purify each siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry and/or analytical HPLC. To provide the utmost in quality, Ambion also assesses each annealed siRNA by gel electrophoresis to confirm that the strands annealed properly. The result is premium quality siRNA that is purified and ready to use.

Approximate turnaround times*

Standard purification 4 business days
HPLC purification 6 business days (20 and 40 nmol)
8 business days (160 nmol)
In Vivo ready 12 business days (100 and 250 nmol)
15 business days (1 µmol)
20 business days (10 µmol)
Cy3 and FAM labels 8 business days

*Estimated time from order confirmation to ship date from Pleasanton, CA for orders to be confirmed by 2pm PST. Does not include time required for shipping (typically overnight for US and Canada deliveries, 2 days for Europe, 2-3 days elsewhere).

Optimized design = guaranteed silencing

Silencer Predesigned and Validated siRNAs are designed for maximum potency and specificity using a proprietary algorithm (see: RNAi has become a powerful tool for understanding gene function). The effectiveness of approximately 1,100 algorithm-designed siRNAs was measured in the silencing of almost 400 endogenously expressed human transcripts. This comprehensive survey indicated that, on a per siRNA basis, more than 80% of the individual siRNAs showed >70% silencing of their target. The performance data clearly confirm the success of the Ambion siRNA selection process.

Bar graph showing % target gene knockdown for targets using silencer predesigned siRNAs

Effectiveness of Silencer Predesigned siRNAs. This graph shows the distribution of gene silencing measured for 808 Silencer Predesigned siRNAs targeting >300 endogenously expressed human genes. Target mRNA levels were measured by qRT-PCR 48 hr after transfection into HeLa cells. More than 82% of the siRNAs successfully silenced their targets by >70%.

Validated Silencer siRNAs

A subset of Silencer siRNAs to human targets have been functionally tested in-house and verified to reduce target mRNA levels by 70% or greater, enabling us to guarantee that these Silencer Validated siRNAs will knock-down their target genes. The data sheet provided with each of these siRNAs shows the extent of mRNA knockdown observed during testing and the exon targeted by the siRNA. For most types, full sequence information is also provided at no additional charge.

For  predesigned & validated siRNAs that incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity, please see Silencer Select Predesigned & Validated siRNAs.

We synthesize and purify each siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry and/or analytical HPLC. To provide the utmost in quality, Ambion also assesses each annealed siRNA by gel electrophoresis to confirm that the strands annealed properly. The result is premium quality siRNA that is purified and ready to use.

Approximate turnaround times*

Standard purification 4 business days
HPLC purification 6 business days (20 and 40 nmol)
8 business days (160 nmol)
In Vivo ready 12 business days (100 and 250 nmol)
15 business days (1 µmol)
20 business days (10 µmol)
Cy3 and FAM labels 8 business days

*Estimated time from order confirmation to ship date from Pleasanton, CA for orders to be confirmed by 2pm PST. Does not include time required for shipping (typically overnight for US and Canada deliveries, 2 days for Europe, 2-3 days elsewhere).

Resources

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Support

If you have any questions on products or ordering, please contact us at RNAiSupport@thermofisher.com