What are Silencer siRNAs?
Silencer siRNA Libraries feature combinations of Silencer Pre-designed siRNAs, and when available, Silencer Validated siRNAs, in 96-well or 384-well plates. The design of each siRNA has been optimized for potent and specific silencing. The siRNA selection process yields siRNAs that are effective at low concentrations, enabling screening experiments at 30 nM siRNA or lower, minimizing reagent costs and off-target effects.
To ensure specificity, the siRNA design algorithm incorporates a rigorous check of all siRNA sequences. This process is accomplished via a modified alignment search tool that effectively identifies short regions of sequence alignment that BLAST searches miss. Sequences that have significant regions of similarity to off-target transcripts, fall in polymorphic regions (e.g., SNPs), or if they are predicted to invoke an antiviral response are eliminated. In addition, the siRNA design process incorporates stringent rules to prevent off-strand targeting.
Pre-defined Silencer siRNA Libraries
Pre-defined Silencer siRNA Libraries are available for the human genome, druggable human genome, and dozens of gene functional classes. They are ready-to-ship versions of our most popular siRNA collections in 96 or 384-well plates. Rat siRNA libraries are also available in this format. See the Silencer Pre-designed siRNAs guarantee.*
NEW Pre-defined Silencer siRNA Libraries
The Silencer Human Genome siRNA Library is ideal for genome-scale surveys of gene function. The Silencer Human Genome siRNA Library is designed for researchers who prefer the use of chemically unmodified but potent siRNA designs that allow highly specific target screens. Three unique, non-overlapping siRNAs are provided for each of 21,686 human targets. This siRNA library is supplied as 0.25 nmol per well in 384-well plates.
The Silencer Human Druggable Genome siRNA Library includes NCBI Entrez gene IDs from the druggable genome that are popular choices for many researchers targeting the most therapeutically relevant human genes. The Silencer Human Druggable Genome siRNA Library targets 7,783 genes and includes three individual siRNAs per gene target, conveniently grouped by target gene class. Please contact us for the complete list of targets. These libraries are available in both 96- and 384-well plate formats. This siRNA library is supplied as 0.25 nmol per well in 384-well plates.
Given their importance in cell signaling and many biological pathways, kinases are key drug targets and subjects of intense scrutiny. The Silencer Human Kinase siRNA Library targets 709 human kinases with three individual Silencer siRNAs per gene. This siRNA library enables systematic yet cost-effective RNAi studies of these key cell regulators. Validated siRNAs are included where available. The Silencer Human Kinase siRNA Library is supplied as 0.25 nmol per well in 96-well plates.
Along with kinases, phosphatases are important regulators of biological pathways and cell signaling cascades. The Silencer Human Phosphatase siRNA Library targets 298 human phosphatases with three individual siRNAs per gene. This library is the ideal companion to the Silencer Human Kinase siRNA Library, enabling more detailed studies of biological pathways. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Human Protease siRNA Library is an ideal solution for focused and cost-effective studies of protease-based functions. This library features three siRNAs per target for 494 human proteases. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
G-protein–coupled receptors, or GPCRs, are key components of many signal transduction pathways and also represent an important class of druggable genes. The Silencer Human GPCR siRNA Library targets 378 non-olfactory human GPCRs with three individual Silencer siRNAs per target supplied in 96-well plates.
The nuclear hormone receptor gene family includes receptors for thyroid and steroid hormones, retinoids, and vitamin D, as well as receptors of unknown ligands. The Silencer Human Nuclear Hormone Receptor siRNA Library V4 is an ideal solution for focused and cost-effective studies of nuclear hormone receptor–based gene knockdown. This library includes three siRNAs per target for 47 nuclear hormone receptors. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
Ion channels are key components in a wide variety of biological processes, including rapid changes in cells such as cardiac, skeletal, and smooth muscle contractions, plus epithelial transport of nutrients and ions, T cell activation, and pancreatic beta-cell insulin release. They are therefore a frequent target for drug discovery. The Silencer Human Ion Channel siRNA Library features three siRNAs per target for 337 human ion channels, providing a cost-effective and focused platform for ion channel gene expression studies. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Human Transcription Factor siRNA Library targets genes expressing proteins that bind DNA in a sequence-specific manner to control the rate of transcription. The Silencer Human Transcription Factor siRNA Library allows potent and specific screening using three siRNAs per target for 1,861 known transcription factors, offering a cost-effective and focused platform for gene regulation screening with highly predictive siRNA design. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Human DNA Damage Response siRNA Library targets genes expressing surveillance proteins that monitor DNA integrity and that can activate cell cycle checkpoints and DNA repair pathways in response to DNA damage, thus preventing potentially deleterious mutations. The Silencer Human DNA Damage Response siRNA Library offers a cost-effective yet potent and specific screening option, using three siRNAs per target for 575 DNA damage gene response targets. This collection, using stringent bioinformatic siRNA design, offers a focused platform to investigate cellular pathways that sense, signal, and repair DNA lesions following DNA damage. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Human Drug Targets siRNA Library includes genes involved with apoptosis, senescence, nucleic acid binding, autophagy, DNA repair, and characterized nuclear receptors, along with other genes that are considered potential targets for therapeutics. This library includes gene IDs from the Thermo Fisher Druggable Genome and Druggable Genome Extension that are not in kinase, phosphatase, protease, ion channel, GPCR, or ubiquitin libraries. The Silencer Human Drug Targets siRNA Library contains three unmodified siRNAs per target for 7,915 genes, designed with stringent bioinformatics to ensure potency and limit off-target effects. This siRNA library offers a comprehensive pre-defined platform for loss-of-function gene studies during therapeutic screens and is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Human Apoptosis siRNA Library V4 provides investigators with a focused siRNA screening platform targeting the most up-to-date apoptosis gene targets and enabling a detailed analysis of the apoptotic signaling pathway. The Silencer Human Apoptosis siRNA Library contains three siRNAs per target for 923 genes. The Silencer Human Apoptosis siRNA Library offers chemically unmodified but highly predictive and potent siRNA designs for confidence in apoptosis screens. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Human Cell Cycle Regulation siRNA Library V4 includes siRNA reagents targeting 1,466 genes whose functions are essential for controlling cell growth and division. The gene targets include cyclin-dependent kinases (CDKs) involved in cell cycle progression, members of the CIP/KIP and INK4 family of cell cycle inhibitors, members of the retinoblastoma protein family, and factors important for DNA replication including cell division cycle proteins (CDCs). Each gene is targeted by three unmodified siRNAs that are derived using stringent bioinformatic design algorithms. These siRNAs possess greater specificity and potency for accurate, consistent, and cost-effective silencing studies of genes critical to cell cycle regulation. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
Ubiquitination is a post-translational modification essential to a wide array of cellular processes, including immunology, apoptosis, biogenesis, DNA transcription and repair, cell cycle and division, cell stress, modulation of cell receptors, and neural and muscular degeneration. The Silencer Human Ubiquitin siRNA Library targets 981 genes, offering a comprehensive set of targets for RNAi-based analysis of the ubiquitin pathway. The Silencer unmodified siRNAs are designed with stringent bioinformatics to minimize off-target effects and provide an accurate yet cost-effective platform for analyzing factors important in the ubiquitin signaling process. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Human Cancer Genome siRNA Library V4 contains gene targets commonly affected in cancers. Often, changes in these genes affect pathways in cells, causing cells to divide and survive when they normally would die. The Silencer Human Cancer Genome siRNA Library contains three siRNAs each targeting 524 cancer-associated genes. The Silencer siRNAs are unmodified yet designed with stringent bioinformatics to avoid off-target effects, allowing accurate and cost-effective RNAi screening in cancer target discovery programs. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
Cell surface receptors (membrane receptors, transmembrane receptors) are integral membrane proteins that take part in communication between cells and their surroundings. Extracellular signaling molecules (usually hormones, neurotransmitters, cytokines, growth factors, or cell recognition molecules) attach to the receptor, triggering changes in the cell. For this process called signal transduction, we have created the Silencer Human Cell Surface siRNA Library, which contains three siRNAs for each of 789 cell surface receptor genes. This library offers a highly potent and cost-effective option for screening with unmodified siRNA designed using stringent bioinformatics to reduce off-target effects and provide accurate data for RNAi-based screens of cell surface signaling factors. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Human Tumor Suppressor siRNA Library contains unmodified siRNAs targeting 725 tumor suppressor genes, or anti-oncogenes, which protect cells on the path to cancer. Mutations that cause loss or reduction in function can result in the progression to cancer, usually in combination with other genetic changes. The Silencer Select Human Tumor Suppressor siRNA Library allows highly robust RNAi-based screening using three siRNAs per gene target that are designed using stringent bioinformatic algorithms. These siRNAs exhibit reduced off-target effects for accurate yet cost-effective RNAi-based silencing of tumor suppressor genes important in cancer research. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Select Human Epigenetics siRNA Library is a comprehensive collection of highly potent, chemically modified siRNAs targeting enzyme classes characterized in epigenetic pathways. These include protein families mediating epigenetic signaling through the acetylation and methylation of histones. The Silencer Select Human Epigenetics siRNA Library is an excellent solution for investigating loss of gene function impact on developmental, disease or evolutionary biology. The Silencer Select Human Epigenetics siRNA Library contains three siRNAs per target for 451 epigenetic enzymes ensuring comprehensive coverage of epigenetic based gene function studies.
Individual siRNAs for enhanced data reliability
Silencer siRNA Libraries feature three or four individual siRNAs for each target. Screening with three or more siRNAs per gene significantly decreases both false positive and false negative rates, resulting in enhanced confidence in RNAi screening data, reduced chance of missing important genes, and less time spent following up on false positive hits from the screen.
These libraries contain 0.25 nmol of each siRNA, sufficient for 100 transfections (at 25 nM siRNA, 100 µL transfection volume). The siRNAs are supplied dried into the wells of 96- or 384-well plates. One column of each 96-well plate or two columns of each 384-well plate are left empty to allow for the addition of controls at the time of use. The libraries are delivered with full siRNA sequence information, as well as the exon targeted and each targeted gene's symbol, full name, aliases, RefSeq number, and locus ID, plus results from any validation experiments performed using the included siRNAs.
Libraries of these or any other list of human, mouse, or rat genes can also be provided with 1, 2, or 5 nmol of each siRNA. Custom formatting is available with these larger sizes. If you would like a gene list or other information, or would like to discuss your research with one of our technical experts, contact your local sales representative or e-mail us at RNAisupport@thermofisher.com
* Guarantee for Silencer™ Pre-designed siRNAs
Life Technologies guarantees that when you purchase three Silencer Pre−designed siRNAs to the same target, at least two of the siRNAs will reduce target mRNA levels in cultured cells by 70% or more when measured 48 hours after transfection at 100 nM or higher final siRNA concentration under the conditions described below. If at least two of the three siRNAs do not induce >70% target mRNA knockdown, Life Technologies will provide a one−time replacement of up to three Silencer Pre−designed siRNAs per target at no additional charge. Requests for replacement product must be made within one hundred and eighty (180) days from the date of delivery of the Silencer Pre−designed siRNAs. Optimum transfection efficiency must be confirmed using good laboratory practices and a proven−to−work siRNA to an endogenous message, such as Ambion Silencer GAPDH siRNA Control. To assess knockdown, target mRNA levels in treated samples must be compared to that of cells transfected with a nontargeting control siRNA, such as Silencer Negative Control #1. We recommend Applied Biosystems TaqMan® Gene Expression Assays to quantify mRNA levels.