• Effective—siRNAs meticulously designed for maximum potency and specificity
  • Convenient—ready-to-use siRNAs in amounts sufficient for hundreds of transfections
  • Reliable—three or more individual siRNAs per target increase screening accuracy, ideal for pathway analysis and target identification and validation
  • Comprehensive—available for small gene sets and for the whole human genome
  • Customizable—custom aliquotting, pooling, and custom libraries available
  • Demonstrated improvements in consistency and reliability of phenotypic result

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What are Silencer® siRNAs?

Silencer® siRNA Libraries feature combinations of Silencer® Pre-designed siRNAs, and when available, Silencer® Validated siRNAs, in 96-well or 384-well plates. The design of each siRNA has been optimized for potent and specific silencing. Indeed, the siRNA selection process yields siRNAs that are effective at low concentrations, enabling screening experiments at 30 nM siRNA or lower, minimizing reagent costs and off-target effects. Learn more about Silencer® siRNAs.

To ensure specificity, the siRNA design algorithm incorporates a rigorous check of all siRNA sequences against the transcriptome of the organism targeted. This process is accomplished via a modified alignment search tool that effectively identifies short regions of sequence alignment that BLAST searches miss. Sequences that have significant regions of similarity to off-target transcripts are eliminated. Also eliminated are sequences that fall in polymorphic regions (e.g., SNPs) and sequences predicted to invoke an antiviral response. In addition, the siRNA design process incorporates stringent rules to prevent off-strand targeting.

For siRNA libraries composed of next-generation pre-designed and validated siRNAs that incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity, please see Silencer® Select siRNA Libraries.

Custom Silencer® siRNA Libraries

The ultimate in flexibility!
Do you have a favorite list of genes you would like to target with a collection of siRNAs? We can prepare a custom Silencer® siRNA Library to any set of human, mouse, or rat genes. The minimum order is only 36 siRNAs. All you need to supply is a list of genes or transcripts identified by NCBI Entrez Gene ID or RefSeq mRNA accession number. With this user-friendly option, you can specify the order of the siRNAs on the plate, as well as the amount of siRNA per order (1 nmol minimum), number of aliquots of each plate, and the number of siRNAs per target. siRNAs can be provided individually or pooled.

Pre-defined Silencer® siRNA Libraries are available for the human genome, druggable human genome, and dozens of gene functional classes. They are ready-to-ship versions of our most popular siRNA collections in 96 or 384-well plates. Rat siRNA libraries are also available in this format. See the Silencer® Pre-designed siRNAs guarantee.*

Human and Mouse Druggable Genome siRNA Libraries
The Silencer® Human Druggable Genome siRNA Library and Silencer® Human Extended Druggable Genome siRNA Library are popular choices for many researchers. Conveniently arranged with siRNAs grouped by gene functional class, these libraries target the most therapeutically relevant genes in the human genome. For those researchers working with mouse cells, we also offer the Silencer® Mouse Druggable Genome siRNA Library, which targets homologs of the genes targeted by the Silencer® Human Druggable Genome siRNA Library. The human and mouse druggable genome siRNA libraries are available in your choice of 96 or 384-well plates.

Human and Mouse Genome siRNA Libraries
Increasingly, researchers are performing genome- scale surveys of gene function. The Silencer® Human Genome siRNA Library and the Silencer® Mouse Genome siRNA Library are ideal for these purposes. The siRNAs to the druggable portion of these libraries are supplied arranged by gene functional class to enable easy screening of important gene subsets such as kinase, phoshpatase, GPCR, etc. The human and mouse genome siRNA libraries are supplied in 384-well plates.

Human and Mouse Kinase siRNA Libraries
Because of their importance in cell signaling and many biological pathways, kinases are key drug targets and subjects of intense scrutiny. The Silencer® Human Kinase siRNA Library targets 719 human kinases with three individual siRNAs per gene. This siRNA library enables systematic, yet cost effective, RNAi studies of these key cell regulators. Validated siRNAs are included for more than half of the genes targeted. A Silencer® Mouse Kinase siRNA Library is also available for use with mouse cells. Both the human and mouse kinase siRNA libraries are provided in 96-well plates.

Human Phosphatase siRNA Library
Along with kinases, phosphatases are important regulators of biological pathways and cell signaling cascades. The Silencer® Human Phosphatase siRNA Library targets 267 human phosphatases with three individual siRNAs per gene supplied in 96-well plates. This library is the ideal companion to the Silencer® Kinase siRNA Library, enabling more detailed studies of biological pathways.

Human GPCR siRNA Library
G-protein–coupled receptors, or GPCRs, are key components of many signal transduction pathways and also represent an important class of druggable genes. The Silencer® Human GPCR siRNA Library targets 381 non-olfactory human GPCRs with 3 siRNAs per target supplied in 96-well plates.

Human Protease, Ion Channel, and Nuclear Hormone Receptor siRNA Libraries
Focused siRNA sets to human proteases, ion channels, and nuclear hormone receptors provide a cost-effective means of studying these important regulators of cell function. These siRNA libraries are supplied in 96-well plates.

Individual siRNAs for enhanced data reliability

Silencer® siRNA Libraries feature three or four individual siRNAs for each target. Screening with three or more siRNAs per gene significantly decreases both false positive and false negative rates, resulting in enhanced confidence in RNAi screening data, reduced chance of missing important genes, and less time spent following up on false positive hits from the screen.

These libraries contain 0.25 nmol of each siRNA, sufficient for 100 transfections (at 25 nM siRNA, 100 µL transfection volume). The siRNAs are supplied dried into the wells of 96 or 384-well plates. One column of each 96-well plate and two columns of each 384-well plate is left empty to allow for the addition of controls at the time of use. The libraries are delivered with full siRNA sequence information, as well as the exon targeted and each targeted gene's symbol, full name, aliases, RefSeq number, and Locus ID, plus results from any validation experiments peformed using the included siRNAs.

Libraries to these or any other list of human, mouse or rat genes can also be provided with 1, 2, or 5 nmol of each siRNA. Custom formatting is available with these larger sizes. If you would like a gene list or other information, or would like to discuss your research with one of our technical experts, contact your local sales representative or e-mail us at RNAilibraries@lifetech.com

*Silencer® Pre-designed siRNAs guarantee

Life Technologies guarantees that when you purchase three Silencer® Pre−designed siRNAs to the same target, at least two of the siRNAs will reduce target mRNA levels in cultured cells by 70% or more when measured 48 hours after transfection at 100 nM or higher final siRNA concentration under the conditions described below. If at least two of the three siRNAs do not induce >70% target mRNA knockdown, Life Technologies will provide a one−time replacement of up to three Silencer® Pre−designed siRNAs per target at no additional charge. Requests for replacement product must be made within one hundred and eighty (180) days from the date of delivery of the Silencer® Pre−designed siRNAs. Optimum transfection efficiency must be confirmed using good laboratory practices and a proven−to−work siRNA to an endogenous message, such as Ambion Silencer® GAPDH siRNA Control. To assess knockdown, target mRNA levels in treated samples must be compared to that of cells transfected with a nontargeting control siRNA, such as Silencer® Negative Control #1. We recommend Applied Biosystems® TaqMan® Gene Expression Assays to quantify mRNA levels.