Collibri DNA Whole Genome Sequencing Kits

Invitrogen Collibri whole-genome library prep kits improve library prep success for high-throughput Illumina™ next-generation sequencing (NGS) systems.

Accommodates a wide range of sample types and inputs:

  • Intact DNA
  • Degraded DNA, including FFPE or cfDNA
  • Suitable for genomes of all sizes
  • Consistent gene coverage from 1 to 500–1,000 ng

Variant detection is consistent among low abundance (1 ng) and high abundance (500–1,000 ng) samples to confidently analyze variable samples.

Sequence SARS-CoV-2 samples

High-quality genomic coverage from nasopharyngeal, oropharyngeal, and diverse samples increases accuracy in SARS-CoV-2 research such as the study of viral evolution and transmission patterns. The optimized workflow for SARS-CoV-2 reduces time and sequencing costs while providing flexibility to use available reagents.

Review the optimized protocol ›


Physically sheared DNA

Sequencing studies can now accept a greater diversity of samples and retain confidence in genomic interpretation. Variant detection rates by the Invitrogen Collibri PS DNA Library Prep Kits for Illumina Systems are similar between amplified (1–100 ng) libraries and PCR-free (500–1,000 ng) libraries.

Physically sheared DNA

Figure 1. Coriell NA12878 DNA was converted into a library and sequenced on an Illumina™ NovaSeq™ 6000 system at 372M reads per sample. SNP recall calculated using FreeBayes v1.2.0.

Enzymatically sheared DNA

Consistency of variant detection provides more consistent, predictable results from diverse samples. Variant detection rates by the Invitrogen Collibri ES DNA Library Prep Kits for Illumina Systems are similar between amplified (100 ng) libraries and PCR-free (100 ng) libraries.

Enzymatically sheared DNA

Figure 2. Coriell NA12878 DNA was converted into a library and sequenced on an Illumina NovaSeq 6000 system at 258M reads per sample. SNP recall calculated using FreeBayes v1.2.0.

Product comparison

  Kit view

Collibri PS DNA Library Prep Kits for Illumina Systems
Kit view

Collibri ES DNA Library Prep Kits for Illumina Systems
PCR-free input 500 ng 100 ng
Input 1–1,000 ng 1–500 ng
Lowest GC bias    
DNA shearing method Physical shearing (e.g., Covaris) Enzymatic shearing
Species supported All All
PCR-free assay time (hr) 1.5 1.5
Assay time (hr) 2.75 2.5
PCR-free hands-on time (hr) 0.5 0.5
Hands-on time (hr) 1 1
Target insert size (bp) 350-550 350-550
All components included    
Visual feedback    
  View performance data View performance data

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