invitrogen collibri library prep kits
  • Ligate adapters, in the form of helper oligos, directly to RNA fragments for best representation of sample diversity
  • Suitable for all sample types, even degraded FFPE
  • Strandedness >98%
  • Total time is up to 50% faster than Illumina, NEB, or Kapa:
    • <6.5 hours for rRNA depletion and library generation
    • <4.5 hours for library generation (without rRNA depletion)

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collibri rna-seq library prep

Collibri Stranded RNA Library Prep Kit for Illumina

  • Low input of 1–25 ng of mRNA-enriched or ribosomal depleted RNA
  • Stranded RNA libraries generated in less than 4.5 hour
collibri stranded rna-seq library prep kit

Collibri Stranded RNA Library Prep Kit for Illumina with H/M/R rRNA Depletion Kit

  • Highly efficient rRNA removal from 100–1000 ng of human, mouse, or rat total RNA
  • Stranded whole-transcriptome libraries generated in less than 6.5 hour
  Collibri Stranded RNA Library Prep Kit for Illumina Collibri Stranded RNA Library Prep Kit for Illumina with H/M/R rRNA Depletion Kit
Hands-on time 1.5 hours 3.5 hours
Total time 4.5 hours 6.5 hours
Input range 1–25 ng* 100–1000 ng
FFPE compatible? Yes Yes
Species compatibility All Human, mouse, rat
* rRNA-depleted or mRNA-enriched RNA

Tips for your RNA-seq library preparation

The scientists who developed the library prep kit share their top hints and tips for success with the Collibri kits.

rRNA removal efficiency

Detect which strand of the genome encodes the gene transcript and differentiate between two distinct alleles encoded on opposite strands of the genome.

Download


Highly efficient depletion of rRNA for human, mouse, and rat samples

Highly efficient rRNA removal from 100–1,000 ng of human, mouse, or rat total RNA for superior detection and coverage of noncoding RNAs.

Highly efficient depletion of rRNA for human, mouse and rat samples

Libraries were prepared from 100 ng of Human Brain Reference RNA according to each manufacturer’s recommended protocol and sequenced on an Illumina HiSeq 4000 system, and then the residual rRNA carryover was measured. Data were normalized to 47 million reads per sample.

tech note on choosing between total rna-seq and mrna-seq

Total RNA-Seq offers the most comprehensive  whole transcriptome analysis. mRNA-Seq is ideal if the research is focused only on the coding region and limited amounts of starting material are available.

Download


Collibri stranded RNA-Seq kits retain sample diversity

RNA-Seq libraries were prepared from 1,000 ng of Human Brain Reference RNA (HBRR) according to manufacturer instructions. Representation of different RNA species was determined by analysis with QoRTs software.

collibri library prep kits retain sample diversity
tech note on understanding NGS library quant options

NGS libraries must be carefully quantified and normalized so that each sample achieves the required number of reads.

Download


NGS library quantification

Collibri Library Quantification Kit

Collibri Library Quantification Kit

  • Accurate quantification of libraries—quantify various sample types, including degraded samples
  • Visual cues—specially formulated dyes to track pipetting steps
  • Platinum hot-start technology—superior specificity, sensitivity, and room temperature reaction setup

NGS library amplification

library amplification master mix with primers

Platinum SuperFi Library Amplification Master Mix with Primer Mix

  • Contains primers that target P5 and P7 regions of Illumina adapters
  • High fidelity—minimal error rates, similar to PCR-free methods
  • Uniform amplification—minimal bias regardless of GC content
library amplification master mix without primers

Platinum SuperFi Library Amplification Master Mix

  • High fidelity—minimal error rates, similar to PCR-free methods
  • Uniform amplification—minimal bias regardless of GC content
  • Master mix—straightforward format provides enhanced ease of use
     


Thermal cyclers

ProFlex 96-well PCR System

  • Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
  • Access the system remotely (and conveniently) through a mobile app
  • Program the instrument in seconds with a simple-to-use touchscreen interface

Veriti 96-Well Fast Thermal Cycler

  • VeriFlex Blocks allow for precise PCR optimization or to run up to six independent assays
  • Easy-to-use graphical interface (6.5-inch VGA Touch Screen) 
  • Reduced PCR cycling time when using faster ramp rates
     


PCR plastics

  • Optimized for performance with Applied Biosystems instruments
  • Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
  • Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
pcr plate without barcodes
adhesive film for applied bio systems pcr plates
8 well PCR tube strips
collibri rna-seq library prep

Collibri Stranded RNA Library Prep Kit for Illumina

  • Low input of 1–25 ng of mRNA-enriched or ribosomal depleted RNA
  • Stranded RNA libraries generated in less than 4.5 hour
collibri stranded rna-seq library prep kit

Collibri Stranded RNA Library Prep Kit for Illumina with H/M/R rRNA Depletion Kit

  • Highly efficient rRNA removal from 100–1000 ng of human, mouse, or rat total RNA
  • Stranded whole-transcriptome libraries generated in less than 6.5 hour
  Collibri Stranded RNA Library Prep Kit for Illumina Collibri Stranded RNA Library Prep Kit for Illumina with H/M/R rRNA Depletion Kit
Hands-on time 1.5 hours 3.5 hours
Total time 4.5 hours 6.5 hours
Input range 1–25 ng* 100–1000 ng
FFPE compatible? Yes Yes
Species compatibility All Human, mouse, rat
* rRNA-depleted or mRNA-enriched RNA

Tips for your RNA-seq library preparation

The scientists who developed the library prep kit share their top hints and tips for success with the Collibri kits.

rRNA removal efficiency

Detect which strand of the genome encodes the gene transcript and differentiate between two distinct alleles encoded on opposite strands of the genome.

Download


Highly efficient depletion of rRNA for human, mouse, and rat samples

Highly efficient rRNA removal from 100–1,000 ng of human, mouse, or rat total RNA for superior detection and coverage of noncoding RNAs.

Highly efficient depletion of rRNA for human, mouse and rat samples

Libraries were prepared from 100 ng of Human Brain Reference RNA according to each manufacturer’s recommended protocol and sequenced on an Illumina HiSeq 4000 system, and then the residual rRNA carryover was measured. Data were normalized to 47 million reads per sample.

tech note on choosing between total rna-seq and mrna-seq

Total RNA-Seq offers the most comprehensive  whole transcriptome analysis. mRNA-Seq is ideal if the research is focused only on the coding region and limited amounts of starting material are available.

Download


Collibri stranded RNA-Seq kits retain sample diversity

RNA-Seq libraries were prepared from 1,000 ng of Human Brain Reference RNA (HBRR) according to manufacturer instructions. Representation of different RNA species was determined by analysis with QoRTs software.

collibri library prep kits retain sample diversity
tech note on understanding NGS library quant options

NGS libraries must be carefully quantified and normalized so that each sample achieves the required number of reads.

Download


NGS library quantification

Collibri Library Quantification Kit

Collibri Library Quantification Kit

  • Accurate quantification of libraries—quantify various sample types, including degraded samples
  • Visual cues—specially formulated dyes to track pipetting steps
  • Platinum hot-start technology—superior specificity, sensitivity, and room temperature reaction setup

NGS library amplification

library amplification master mix with primers

Platinum SuperFi Library Amplification Master Mix with Primer Mix

  • Contains primers that target P5 and P7 regions of Illumina adapters
  • High fidelity—minimal error rates, similar to PCR-free methods
  • Uniform amplification—minimal bias regardless of GC content
library amplification master mix without primers

Platinum SuperFi Library Amplification Master Mix

  • High fidelity—minimal error rates, similar to PCR-free methods
  • Uniform amplification—minimal bias regardless of GC content
  • Master mix—straightforward format provides enhanced ease of use
     


Thermal cyclers

ProFlex 96-well PCR System

  • Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
  • Access the system remotely (and conveniently) through a mobile app
  • Program the instrument in seconds with a simple-to-use touchscreen interface

Veriti 96-Well Fast Thermal Cycler

  • VeriFlex Blocks allow for precise PCR optimization or to run up to six independent assays
  • Easy-to-use graphical interface (6.5-inch VGA Touch Screen) 
  • Reduced PCR cycling time when using faster ramp rates
     


PCR plastics

  • Optimized for performance with Applied Biosystems instruments
  • Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
  • Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
pcr plate without barcodes
adhesive film for applied bio systems pcr plates
8 well PCR tube strips

View sample data sets for whole transcriptome RNA sequencing

Investigate sequencing data…before you sequence

It can be time-consuming to evaluate an exciting new library preparation method. Accelerate the evaluation process by analyzing data sets that have already been sequenced on an Illumina HiSeq 4000 system.

Data sets are available for the following sample types and methods:

Sample type Input (ng) Method Library preparation kit Instrument Read lenght (bp)
Human Brain Reference RNA (HBRR) 100 ng of total RNA Whole transcriptome sequencing Collibri Stranded RNA Library Prep for Illumina systems with H/M/R rRNA Depletion Kit Illumina HiSeq 4000 system 2 x 75
Universal Human Reference RNA (UHRR) 100 ng of total RNA Whole transcriptome sequencing Collibri Stranded RNA Library Prep for Illumina systems with H/M/R rRNA Depletion Kit Illumina HiSeq 4000 system 2 x 75

How to download data sets from Thermo Fisher Cloud

Data sets may be downloaded at no charge from the Thermo Fisher Cloud in six easy steps.

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Image of gene expression selection
Image of gene expression selection


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