Reduce the cost of NGS library prep through miniaturization

Invitrogen Collibri next-generation sequencing (NGS) libraries prepared on the SPT Labtech mosquito HV automation platform provide reliable statistical power to interpret results and reduce NGS library prep costs by 6-fold.

  • Miniaturized reagent volumes reduce the cost of NGS library prep by 6-fold compared to manual protocols and traditional automated protocols
  • Consistent, reliable variant detection from as little as 1 ng of DNA
  • Improved sequencing success  through the preparation of sample replicates at no additional preparation cost

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Reduce the cost of NGS library prep compared to traditional automation

The mosquito and dragonfly automation systems from SPT Labtech efficiently generate NGS libraries at a fraction of the traditional volumes, up to a 10-fold reduction. Miniaturization provides cost savings while maintaining the quality of the Collibri libraries for Illumina NGS systems.

Save time by using automated scripts for the Collibri portfolio of library prep kits and accessories, developed by SPT Labtech.

For users requiring higher throughput the dragonfly discovery can be used alongside the mosquito HV for all reagent dispensing steps. The dragonfly discovery enables rapid non-contact dispensing of reagents whilst also significantly reducing plastic consumable consumption.

Collibri ES DNA Library Prep Kit for Illumina Systems
Supported, documentation available
Dispensing steps supported
Collibri Library Quantification Kit
Push-button setup using the wizard
Dispensing steps supported

Reliable, consistent variant detection from 1 ng of DNA

When automating NGS library prep methods, it is critical to maintain even coverage and minimize bias. GC bias is the biggest contributor to uneven coverage, but it can be minimized by using an optimal PCR master mix or PCR-free library preparation methods. Collibri DNA library preparation kits contain Collibri Library Amplification Master Mix to enable uniform coverage across a wide range of GC content. Representative fastq files are available via Connect, our cloud-based platform, for analysis with your analytical pipeline.

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Physically sheared DNA
Figure 1. Call variants with confidence. Coriell NA12878 DNA was converted into a library using the Collibri PS DNA Library Prep Kit for Illumina Systems and sequenced on an Illumina™ NovaSeq™ 6000 system at 372M reads per sampleSNP recall calculated using FreeBayes v1.2.0.

Improved sequencing success rates

Miniaturization decreases the volume of NGS library prep reagents by as much as 10-fold, enabling 5–6 replicates of each sample to be generated for less than the cost of a single sample using traditional automation. Sequencing 5-6 replicates per sample removes the need to repeat the library preparation process or sequencing run if a single sample is not successfully sequenced. The likelihood of a sample being successfully sequenced the first time is therefore improved.

Dr. Stuart Levine

Increasing NGS experimental power without increasing costs

Dr. Stuart Levine, Director of the BioMicro center at MIT, has developed methodologies to dramatically lower NGS library prep costs without sacrificing data quality. Performance data from the Collibri ES DNA Library Prep Kits 
demonstrates 10–15 fold reduction in costs.

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Download Collibri ES DNA Library Prep Kit User Guide

Visual feedback at each step during NGS library prep confirms well-to-well consistency

Each reagent in the Collibri portfolio of NGS library prep kits and accessories contains a tracking dye to visualize library prep progress. When reagents are thoroughly mixed, the solutions change color to indicate proper library preparation technique. Each step in the library prep process contains an internal control in the form of reagent colors, improving the success rate of NGS library generation. The tracking dyes are removed prior to library quantification and do not interfere with sequencing.

For Research Use Only. Not for use in diagnostic procedures.