Sanger sequencing is used to study a small subset of genes linked to a defined phenotype, confirm next-generation sequencing (NGS) variants, detect minor allele fractions down to 5%, or read contiguous sequences up to 1,000 bases. 

Fragment analysis is a powerful technique with simple, straightforward workflows and used in a wide-range of applications, such as detection of mutations, genotyping, identification of short tandem repeats, and gene expression profiling.

Applied Biosystems genetic analysis systems are a trusted standard for Sanger sequencing and fragment analysis by capillary electrophoresis—proven through decades of results, including the first sequencing of the human genome and the discovery of genes implicated in diseases like cystic fibrosis. 

Explore applications, instrumentation, reagents, consumables, and software designed to respond to the unlimited potential of scientific inquiry.

Solutions for Sanger sequencing and fragment analysis
SeqStudio Genetic Analyzer
Capillary Electrophoresis Reagents
Sanger Sequencing and Fragment Analysis Software

Learn more about each step of the recommended
workflow, from DNA and RNA isolation and PCR amplification to capillary electrophoresis and
data analysis

Learn how fluorescently labeled DNA fragments are separated using capillary electrophoresis (CE) and sized by comparison to a size standard

Sanger sequencing is the gold standard for sequencing technology since it provides a high degree of accuracy, long-read capabilities, and the flexibility to support a diverse range of applications in many research areas

Learn more about the applications fragment analysis enables, from microsatellite marker analysis in cell line authentication (CLA) to multiplex ligation-dependent probe amplification (MLPA) assay in inherited disease research

Find out how you can manage workflows and track genomic data for Sanger sequencing and fragment
analysis in one platform

Scientist Spotlights

Learn how scientists are finding answers to complex and challenging questions through perseverance and technology.


Lillian Seu, PhD

Centre for Infectious Disease Control, Zambia (CIDRZ)

Using Sanger Sequencing to Detect Drug Resistance Mutations in HIV in Zambia


Stuart Adams, PhD

Great Ormond Street Hospital for Children NHS Foundation Trust

Sanger Sequencing and Fragment Analysis Help Improve Clinical Outcomes of Acute Lymphoblastic Leukemia (ALL) in Pediatric Patients

Build your knowledge

Build your knowledge

Get bite-sized answers to your everyday Sanger sequencing and fragment analysis questions

Learn about the history of sequencing and how to pick the right platform for your research needs