Sanger sequencing is used to study a small subset of genes linked to a defined phenotype, confirm next-generation sequencing (NGS) variants, detect minor allele fractions down to 5%, or read contiguous sequences up to 1,000 bases.
Fragment analysis is a powerful technique with simple, straightforward workflows and used in a wide-range of applications, such as detection of mutations, genotyping, identification of short tandem repeats, and gene expression profiling.
Applied Biosystems genetic analysis systems are a trusted standard for Sanger sequencing and fragment analysis by capillary electrophoresis—proven through decades of results, including the first sequencing of the human genome and the discovery of genes implicated in diseases like cystic fibrosis.
Sanger sequencing is the gold standard for sequencing technology since it provides a high degree of accuracy, long-read capabilities, and the flexibility to support a diverse range of applications in many research areas
Learn more about the applications fragment analysis enables, from microsatellite marker analysis in cell line authentication (CLA) to multiplex ligation-dependent probe amplification (MLPA) assay in inherited disease research
Learn more about each step of the recommended
workflow, from DNA and RNA isolation and PCR amplification to capillary electrophoresis and
Learn how fluorescently labeled DNA fragments are separated using capillary electrophoresis (CE) and sized by comparison to a size standard
Learn how scientists are finding answers to complex and challenging questions through perseverance and technology.
University of Tennessee Health Science Center (UTHSC)
Breast Cancer Disparity Among African-American Women: Insights Powered by Sanger Sequencing
For Research Use Only. Not for use in diagnostic procedures.