Gibco Essential 8 Flex Medium is formulated to extend the activity of key heat-sensitive components found in PSC medium, including FGF2, to enable truly weekend-free culture with a flexible feeding schedule.
- Flexible feeding schedule—maintain pluripotency over a full 2-day period without feeding cells
- Robust cultures—optimized stability of heat sensitive components, like FGF2, increases maintenance of pluripotency
- Easy to transition—same setup, applications and protocol as the original Essential 8 Medium formulation, but without the daily feeding
- Proven—based on the original Gibco Essential 8 Medium formulation, maintains pluripotency and normal karyotypes in long-term culture
Gibco PSC Media are manufactured according to cGMP for medical devices, 21 CFR Part 820
Routine PSC expansion and maintenance without the need for daily feeding
Essential 8 Flex Medium is a serum-free, xeno-free medium that supports the culture and expansion of pluripotent stem cells (PSCs) while eliminating the need for daily feeding schedules (Figure 1). Just like the original Essential 8 Medium formulation, the Essential 8 Flex Medium is xeno-free and does not include BSA (bovine serum albumin) or HSA (human serum albumin) components, helping to minimize batch variability and maintain long-term health and pluripotency of PSCs (Figures 2–3).
Figure 1. Culture schedule comparison. Unlike other feeder-free media, Gibco Essential 8 Flex Medium eliminates the need to manage cultures daily, enabling a truly weekend-free schedule for expansion and maintenance of PSCs. The Essential 8 Flex medium workflow can be adjusted to a once-per-week passage if required to fit existing workflows. Simply change day 1 to a feed day in the workflow above.
Robust pluripotent stem cell cultures
FGF2 levels widely fluctuate in standard PSC culture medium, even with daily feeding (Figure 4). The use of Essential 8 Flex medium significantly limits these fluctuations, producing more robust, stable culture conditions with the added value of a flexible feeding schedule.
Figure 4. Essential 8 Flex medium limits fluctuations in levels of active FGF2. Measurements of heat-mediated loss of active FGF2 were used to simulate active FGF2 levels in PSC cultures over the course of a week. Standard PSC culture medium (red curve) cultures would be fed on a daily basis while Essential 8 Flex medium (blue curves) cultures would be follow the suggested protocol outlined in Figure 1.
Complete media system
The following components are recommended to create a complete media system for weekend-free routine expansion and maintenance of PSCs with Essential 8 Flex Medium.
Webinar: Essential 8 Flex: Eliminating daily feeding in a feeder-free, xeno-free PSC culture system
For Research Use Only. Not for use in diagnostic procedures.