Gibco Essential 8 Flex Medium is formulated to extend the activity of key heat-sensitive components found in PSC medium, including FGF2, to enable truly weekend-free culture with a flexible feeding schedule.
- Flexible feeding schedule—maintain pluripotency over a full 2-day period without feeding cells
- Robust cultures—optimized stability of heat sensitive components, like FGF2, increases maintenance of pluripotency
- Easy to transition—same setup, applications and protocol as the original Essential 8 Medium formulation, but without the daily feeding
- Proven—based on the original Gibco Essential 8 Medium formulation, maintains pluripotency and normal karyotypes in long-term culture
Returning to the lab?
Maximize your time with Essential 8 Flex Medium
Essential 8 Flex Medium is designed to eliminate the need to manage cultures daily which allows for more flexible feeding schedules, including up to two consecutive feed-free days, so that your cultures can be adapted to your schedule.
Gibco PSC Media are manufactured according to cGMP for medical devices, 21 CFR Part 820
Routine PSC expansion and maintenance without the need for daily feeding
Essential 8 Flex Medium is a serum-free, xeno-free medium that supports the culture and expansion of pluripotent stem cells (PSCs) while eliminating the need for daily feeding schedules (Figure 1). Just like the original Essential 8 Medium formulation, the Essential 8 Flex Medium is xeno-free and does not include BSA (bovine serum albumin) or HSA (human serum albumin) components, helping to minimize batch variability and maintain long-term health and pluripotency of PSCs (Figures 2–3).
Figure 1. Culture schedule comparison. Unlike other feeder-free media, Gibco Essential 8 Flex Medium eliminates the need to manage cultures daily, enabling a truly weekend-free schedule for expansion and maintenance of PSCs. The Essential 8 Flex medium workflow can be adjusted to a once-per-week passage if required to fit existing workflows. Simply change day 1 to a feed day in the workflow above.
Robust pluripotent stem cell cultures
FGF2 levels widely fluctuate in standard PSC culture medium, even with daily feeding (Figure 4). The use of Essential 8 Flex medium significantly limits these fluctuations, producing more robust, stable culture conditions with the added value of a flexible feeding schedule.
Figure 4. Essential 8 Flex medium limits fluctuations in levels of active FGF2. Measurements of heat-mediated loss of active FGF2 were used to simulate active FGF2 levels in PSC cultures over the course of a week. Standard PSC culture medium (red curve) cultures would be fed on a daily basis while Essential 8 Flex medium (blue curves) cultures would be follow the suggested protocol outlined in Figure 1.
Complete media system
The following components are recommended to create a complete media system for weekend-free routine expansion and maintenance of PSCs with Essential 8 Flex Medium.
Webinar: Essential 8 Flex: Eliminating daily feeding in a feeder-free, xeno-free PSC culture system
For Research Use Only. Not for use in diagnostic procedures.