siRNA Screening Libraries

Scale confidently with customizable, high-potency siRNA libraries. High-throughput screens harness the power of RNAi to better understand gene function and biological pathways. Thermo Fisher Scientific offers predefined and custom sets of potent siRNAs for arrayed functional genomics studies. Each of our siRNA libraries is backed by a silencing guarantee, helping give you confidence in the hits you identify and supporting reliable results.

We offer Silencer Select and Silencer siRNA libraries, as either predefined or custom libraries. All our libraries are included with full siRNA annotation files to include:

• Plate map ID and well address for each siRNA
• siRNA sequence information
• NCBI identifiers: gene symbol, gene name, alias(es), Entrez gene ID, and associated RefSeq mRNA accession numbers.
• Plates are individually barcoded with unique identifiers

Silencer Select siRNA is our most potent and specific siRNA available due to a next-generation algorithm and patented chemical modifications. 

Available Silencer Select siRNA library options include:

• Human pre-defined gene families and pathways, including the druggable genome
• Human whole genome siRNA library
• Human genome-wide siRNA collection to target long-noncoding genes

Backed by one of the best silencing guarantees in the industry, our Silencer Select siRNA libraries are designed to be reliable, efficient and specific for clear and consistent siRNA screening results. 

Silencer siRNA is a great choice for cost-effective screening of pre-defined gene family subsets and popular pathways to accelerate your functional genomics studies. These unmodified siRNA screening libraries are built with our Silencer predesign inventory and achieve good silencing at a low cost.

Since 2010, Silencer Select siRNA libraries have been enabling functional genomics studies in labs around the world. These selected peer-reviewed references showcase the power of our high-quality siRNAs in high-throughput screening applications.

• Hasson S, Kane L, et al. (2013) High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy. Nature 504: 291-295. 
  This Nature publication demonstrates the utility of the Silencer Select genome-wide siRNA library for arrayed high-throughput screening in effectively identifying novel drug targets for Parkinson’s disease.
• Shum D, Bhinder B, et al. (2013) An arrayed RNA interference genome-wide screen identifies candidate genes involved in the MicroRNA 21 biogenesis pathway. Assay Drug Dev Technol 11: 191-205. 
  Researchers at Memorial Sloan Kettering uncover a number of novel candidate regulators of microRNA function using the Silencer Select whole genome siRNA library.
• Sivan G, Ormanoglu P, et al. (2015) Identification of Restriction Factors by Human Genome-Wide RNA Interference Screening of Viral Host Range Mutants Exemplified by Discovery of SAMD9 and WDR6 as Inhibitors of the Vaccinia Virus K1L−C7L− Mutant. mBio 6: e01122-15. 
  Genome-wide screen results demonstrate higher phenotypic penetrance from multiple individual Silencer Select siRNAs than with a pooled siRNA reagent from another vendor.
• Lingeman M, McCarty T, et al. (2019) The alpha-1 subunit of the Na+,K+-ATPase (ATP1A1) is required for macropinocytic entry of respiratory syncytial virus (RSV) in human respiratory epithelial cells. PLoS Pathog 15: e1007963. 
  A genome-wide Silencer Select siRNA library screen uncovers a model for RSV entry into human airway epithelial cells requiring activation of ATP1A1, signaling it as a potential new target for the development of anti-RSV drugs.
• Kainulainen, V., von Schantz-Fant, C., et al. (2022) Genome-wide siRNA screening reveals several host receptors for the binding of human gut commensal Bifidobacterium bifidum. npj Biofilms Microbiomes 8, 50 
  Genome-wide Silencer Select screen reveals cell surface proteins involved in B. bifidium-host cell interactions.
• Shrestha , R., Balachandra, V., et al. (2023) The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability. J Cell Sci 136: 10 
  The NIH defines gene roles in preventing CENP-A mislocalization and chromosomal instability using an imaging-based high-throughput siRNA screen with a Silencer Select Epigenetics library.
• Morimoto, K., Yamada, T., et al. (2024) AXL signal mediates adaptive resistance to KRAS G12C inhibitors in KRAS G12C-mutant tumor cells. Cancer Letters 587: 216692 
  Applying the Silencer Select Kinase library, researchers investigate the mechanism for adaptive resistance to KRAS G12C inhibitors for treatment in lung cancer.
• Kolapalli, S., Beese , C., et al. (2025) Pellino 3 E3 ligase promotes starvation-induced autophagy to prevent hepatic steatosis. Sci Adv 11, eadr2450 
  Researchers use the Silencer Select Ubiquitin library to identify crucial genes involved in regulating starvation-induced autophagy with high-content imaging.