Two mate-paired library construction methods are available. The SOLiD® Long Mate-Paired Library Construction Kit uses a protocol that captures ~50 bp on either end of the circularized fragment and uses a time-course S1 Nuclease digestion of the DNA (Figure 1). The SOLiD® 2 x 25 bp Mate-Paired Library Construction Kit uses a protocol that captures ~25 bp of either end of the circularized fragment and uses a restriction digestion with EcoP15 to generate the fragments (Figure 2). In general, capture of longer ends is preferred to provide as much information as possible and facilitate bioinformatic mapping. However, for several reasons including biases of each method, consistency of cuts, prior research, and organisms of interest, it may be desirable to generate libraries using both protocols.
The SOLiD® Mate-Paired Library Construction Kits:
- Enable a convenient and simplified workflow — includes all reagents, columns, and gels for the construction of ten libraries (see note)
- Optimized and recommended protocols for use with the SOLiD® System
- Components quality-tested together to help ensure optimal results