Sequence_Researcher

From research to the clinic, single gene to small gene panels to exomes or transcriptomes, our sequencing & fragment analysis solutions—using CE or NGS—help you master your sequence across a diverse set of applications.

Next-generation sequencing (NGS)


Sanger sequencing/fragment analysis

Molecular profiling of cancers is becoming more and more important in the research area of personalized cancer therapy, or precision oncology. NGS provides a valuable method for high-throughput applications when many targets and samples need to be multiplexed and screened. However, for very focused applications such as single-analyte or single-gene targets, there is a clear need for fast, simple, and affordable methods for detecting low-level somatic variants. The improved sensitivity achieved using Minor Variant Finder Software makes Sanger sequencing an ideal technology for filling this gap.

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Formalin-fixed paraffin-embedded (FFPE) tissue is a standard sample type in histology and pathology laboratories; however, the fixation process often damages DNA, resulting in a limited amount of starting material for molecular genetic analysis. In order to extract maximal information from minimal sample amounts, we developed a workflow that enables robust genotyping results from less than 1 ng of FFPE DNA. Utilizing Ion AmpliSeq technology with Sanger sequencing, we offer an ideal solution for routine labs working with a limited number of samples/targets, as well as high-throughput labs that need an orthogonal method for confirming minor alleles.

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The Applied Biosystems HIV-1 Genotyping Kit harnesses gold-standard Sanger sequencing technology to amplify and reliably sequence the diverse and rapidly evolving HIV-1 virus. The kit enables reliable genotyping of the genetically diverse HIV-1 virus from plasma and dried blood spot (DBS) samples, to detect resistance to protease inhibitors, nucleoside reverse-transcriptase inhibitors, and non-nucleoside reverse-transcriptase inhibitors.

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Microsatellite analysis includes PCR amplification of the microsatellite loci using fluorescently labeled primers; labeled PCR products are then analyzed by capillary electrophoresis (CE) or electrophoresis to separate the alleles by size. We offer a broad range of reagents, kits, instruments, and software that will help you achieve consistently precise and reliable results for a multitude of research applications.

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