Sequence_Researcher

From research to the clinic, single gene to small gene panels to exomes or transcriptomes, our sequencing & fragment analysis solutions—using CE or NGS—help you master your sequence across a diverse set of applications.

Next-generation sequencing (NGS)


Sanger sequencing/fragment analysis

Molecular profiling of cancers is becoming more and more important in the research area of personalized cancer therapy, or precision oncology. NGS provides a valuable method for high-throughput applications when many targets and samples need to be multiplexed and screened. However, for very focused applications such as single-analyte or single-gene targets, there is a clear need for fast, simple, and affordable methods for detecting low-level somatic variants. The improved sensitivity achieved using Minor Variant Finder Software makes Sanger sequencing an ideal technology for filling this gap.

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Formalin-fixed paraffin-embedded (FFPE) tissue is a standard sample type in histology and pathology laboratories; however, the fixation process often damages DNA, resulting in a limited amount of starting material for molecular genetic analysis. In order to extract maximal information from minimal sample amounts, we developed a workflow that enables robust genotyping results from less than 1 ng of FFPE DNA. Utilizing Ion AmpliSeq technology with Sanger sequencing, we offer an ideal solution for routine labs working with a limited number of samples/targets, as well as high-throughput labs that need an orthogonal method for confirming minor alleles.

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Human sample identification can be defined as three applications: human cell line authentication, sample authentication, and mixed sample analysis. Short tandem repeat (STR) genotyping is an important tool in verifying the authenticity of human cell lines, quality control of stored human tissues and fluids, and assessing the nature of known mixtures.

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For Research Use Only. Not for use in diagnostic procedures.