Technical and educational videos for flow cytometry topics

flow cytometry educational videos and webinars

These videos, tutorials and webinars are designed to provide technical and education information regarding flow cytometers as well as flow cytometry research applications and techniques.


Featured videos

Dr Prussak

The complex pharmacology of T-cell CARs

In this webinar, Charles Prussak, PharmD, PhD., Director of the Cell Therapy Translational Laboratory (CTTL), UCSD, discusses the generation CAR-T therapies, toxicities that are most commonly observed when employing CD-19 directed CAR-T therapies, and the next generation of CAR-T cells being created and clinically tested for the treatment of both hematological and solid tumor malignancies.

CYToMORE 2016—Advances in cytometry

The 6th Modena Advanced Course in Cytometry (CYToMORE), was aimed at researchers who wanted to improve their flow cytometry knowledge and learn new applications. Organized in collaboration with the Italian Society of Immunology, Clinical Immunology and Allergology (SIICA) and led by teachers who are among the top international experts in the field, 2016 was a huge success. In this presentation, Victor Appay, Faculté de Médecine Pierre et Marie Curie, at Pitié-Salpêtrière, France discusses his work on “Assessing immunological aging using flow cytometry”.


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Type Title and description Categories
CYToMORE lecture video (2016) ...and on the 8th day, God created cytometry 
Presented by J. Paul Robinson, Purdue University, USA
flow cytometers, flow cytometry, history, technology developments
CYToMORE lecture video (2016) Assessing immunological aging using flow cytometry 
Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France
flow cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016) Asymptomatic but threatening: dissecting the consequences of cytomegalovirus infection 
Presented by Anis Larbi, Singapore Immunology Network – SigN, Singapore
flow cytometry assays, immunology
CYToMORE lecture video (2016) Cancer stem cells and multi-drug resistance by flow cytometry 
Presented by Jordi Petriz, Josep Carreras Leukaemia Research Institute, IJC, Spain
cancer, flow cytometry, multidrug resistance (MDR), stem cell research
CYToMORE lecture video (2016) Cytometric analysis of cytokines and transcription factors 
Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany
cell signaling, flow cytometry, immunophenotyping
CYToMORE lecture video (2016) Cytometry of immunity 
Presented by Andreas Radbruch, Deutsches Rheuma-Forschungszentrum Berlin, Germany
flow cytometry, immunology
CYToMORE lecture video (2016) Cytomic analysis of oxidative stress 
Presented by J. Enrique O’Connor, University of Valencia, Spain
flow cytometry, oxidative stress
CYToMORE lecture video (2016) High throughput and high content flow cytometry 
Presented by J. Paul Robinson, Purdue University, USA
Attune NxT, flow cytometer, high content flow cytometry, high throughput screening
CYToMORE lecture video (2016) Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy 
Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy
flow cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016) Host-microbe interaction at mucosal surfaces 
Presented by Maria Rescigno, Istituto Europeo di Oncologia – IEO, Italy
flow cytometry, immunology, microbiology
CYToMORE lecture video (2016) Mass cytometry in the phenomics era: an essential tool for gene/function studies 
Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France
mass cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016) Microenvironment cytometry 
Presented by Paul Smith, Cardiff University, UK
cancer, cell cycle, flow cytometry, tumor microenvironments, drug discovery
CYToMORE lecture video (2016) Novel reagent solutions for flow cytometry 
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune NxT, flow cytometry assays
CYToMORE lecture video (2016) Rare cells analysis: from bench to clinics 
Presented by Andrea Cossarizza, University of Modena and Reggio Emilia, Italy
Attune NxT, flow cytometer, rare cell detection, rare event detection
CYToMORE lecture video (2016) RNA flow cytometry 
Presented by Paul Wallace, Roswell Park Cancer Institute, USA
flow cytometry, in situ hybridization, RNA
CYToMORE lecture video (2016) Software tutorial: what’s new 
Presented by Carmen Gondhalekar, Purdue University, USA
Attune NxT, flow cytometer, software
CYToMORE lecture video (2016) Tuning acoustic forces for flow cytometry 
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune NxT, flow cytometer
How-to video Tutorial: Labeling a purified antibody 
Educational video on how to label your next antibody for imaging or fluorescence. Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield.
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
How-to video How to site specifically label your antibody using SiteClick technology 
This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SiteClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain.
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection
Technology video Click-iT Plus EdU Proliferation Assay 
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging
Technology video The Two Worlds of Cell Separation cell isolation, immunology
Tutorial video Molecular Probes Tutorial Series—Analyzing Flow Cytometry Data flow cytometer calibration, flow cytometry
Tutorial video Molecular Probes Tutorial Series—Anatomy of Fluorescence Spectra absorbance, emission, excitation, spectra
Tutorial video Molecular Probes Tutorial Series—Introduction to Flow Cytometry flow cytometer calibration, multicolor flow cytometry
Tutorial video Molecular Probes Tutorial Series—Introduction to Fluorescence flow cytometry, fluorescence imaging
Tutorial video Molecular Probes Tutorial Series—Overview of Filters and Light Sources emission, excitation, filters, light cubes, light sources
Webinar The Complex Pharmacology of T-Cell CARs 
In this webinar, Charles Prussak, PharmD, PhD., Director of the Cell Therapy Translational Laboratory (CTTL), UCSD, discusses the generation CAR-T therapies, toxicities that are most commonly observed when employing CD-19 directed CAR-T therapies, and the next generation of CAR-T cells being created and clinically tested for the treatment of both hematological and solid tumor malignancies.
flow cytometry, immuno-oncology
Webinar A practical approach to antibody labeling 
The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will:
  • Provide an overview of our antibody labeling kits
  • Offer guidance on which methods are ideal for specific applications and experiments
  • Provide tips and tricks to optimize your labeling protocol
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis 
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability
Webinar An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis 
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability
Webinar Basics of flow cytometry, Part I: Gating and data analysis 
An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry.
flow cytometry data, flow cytometry set up, multicolor flow cytometry
Webinar Basics of flow cytometry, Part II: Compensation 
This presentation provides an overview of basic fluorochromes used in flow cytometry. Topic includes: the principle of compensation, how to perform compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation.
flow cytometry data, flow cytometry set up, multicolor flow cytometry
Webinar Basics of multi color flow cytometry panel design 
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including:
  • Rules for designing panels
  • Examples and practical application of these rules
  • Controls and standardization
  • Relevance of panel design to new mass cytometry platforms
antibodies, compensation, multicolor flow cytometry,
Webinar DNA content cell cycle analysis using flow cytometry 
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss:
  • An overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content
  • Selection of DNA dyes for live cell and fixed cell analysis
  • Tips and tricks for consistent results
cell counting, cell cycle, cell proliferation, flow cytometry
Webinar Flow cytometry in microbiological research 
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
microbiology, multicolor flow cytometry, viability
Webinar Learn to choose the right fluorophore when designing experiments 
The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore:
  • How to choose the best organic dye for an assay
  • Quantum dots and how they compare to other dyes
  • When to use a phycobiliprotein like R-PE or APC
  • When to use fluorescent proteins like GFP
  • How to choose a suitable dye to match your instrument
In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs.
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Webinar How recent technological advances in flow cytometry instrumentation are enabling faster throughput 
This webinar presented by J. Paul Robinson at Purdue University, will focus on advances in the Invitrogen Attune NxT Flow Cytometer and outline where the technology within the Attune NxT instrument fits in current and future applications.
Attune NxT, cell cycle, multicolor flow cytometry
Webinars (ongoing) Protein and cell analysis upcoming educational webinars 
See our upcoming webinars and see what is now available on demand in our virtual learning platform.
education