Silencer siRNAs

Invitrogen Silencer predesigned siRNAs are available for all human, mouse, and rat gene targets in the RefSeq database. These unmodified siRNAs are designed for maximum potency and specificity using a highly effective and extensively tested algorithm. Each siRNA is synthesized to the highest quality standards and is offered with full sequence information.

When you purchase three predesigned Silencer siRNAs to the same target, we guarantee that at least two of the siRNAs will achieve >70% reduction in target mRNA levels. Visit our siRNA guarantee page for more information.

Order custom designed silencer siRNA
Order Silencer siRNA positive and negative controls

A subset of Silencer siRNAs to human targets have been functionally tested in-house and verified to reduce target mRNA levels by 70% or greater, enabling us to guarantee that these Silencer siRNAs will knock-down their target genes. The data sheet included with each of these siRNAs shows the extent of mRNA knockdown observed during testing and the exon targeted by the siRNA. For most types, full sequence information is also included at no additional charge.

For predesigned and verified siRNAs that incorporate our latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with excellent efficacy, potency, and specificity, please see Silencer Select Predesigned siRNAs.

Silencer predesigned and verifed siRNAs are designed for maximum potency and specificity using a proprietary algorithm. The effectiveness of approximately 1,100 algorithm-designed siRNAs was measured in the silencing of almost 400 endogenously expressed human transcripts. This comprehensive survey indicated that, on a per siRNA basis, more than 80% of the individual siRNAs showed >70% silencing of their target. The performance data confirm the success of the Ambion siRNA algorithm.

We synthesize and purify each siRNA in advanced facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry and/or analytical HPLC. To help ensure the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands annealed properly. The result is premium quality siRNA that is purified and ready to use.

Order Silencer siRNA libraries