Transfection basics

The Transfection Basics resource collection provides an overview of transfection, including general information on various transfection technologies, considerations for successful transfection experiments, and selecting the appropriate transfection method for your cell line and experimental needs.

Use this transfection product family selection guide to choose the solution that's right for you.

Download the selection guide


Application notes

Transfection application notes present research applications and proof-of-concept experiments involving Thermo Scientific, Invitrogen, and Gibco products from our cell biology portfolio.

If you're looking to learn about methods and techniques to achieve improved transfection, tested protocols, or descriptions of various techniques (and their trade-offs), you are likely to find such answers among these application notes.


Neurobiology transfection guide


Delivery solutions for immune cells


LentiVirus production


  Optimizing cardiomyocyte transfection with Lipofectamine MessengerMAX reagent


Improve genome editing outcomes In biologically relevant cell models


  Generation and transfection of induced pluripotent (iPSCs)


  Versatility and performance of Lipofectamine Stem Transfection Reagent in Stem Cells

Virtual lab

Virtual lab

The “Cell Culture Basics—Transfection” virtual training lab is an introduction to transfection covering many topics, including:

  • Overview of common transfection delivery methods
  • Conducting positive and negative controls
  • Performing a lipid-based transfection
  • Performing an electroporation transfection
  • Analyzing cells

Explore the lab

The Gibco cell culture basics—transfection lab is endorsed by The American Association for Cell Biology (ASCB). Gibco and ASCB are committed to cell biology education. As part of our partnership you will receive discounted membership to ASCB after completing the virtual lab.

ASCB logo

ASCB logo


Transfection handbook

This handbook outlines a collection of superior transfection and genome engineering products designed to work together to help answer research questions faster and with less effort.

Download now

Transfection protocol calculator

Let us take the stress and hassle out of scaling your experiment. Get your customized transfection protocol by entering just a few details.

Get your protocol


Transfection protocols

all protocols

Transfection Protocols (all)

protocols by cell type

Transfection Protocols (by cell type)

protocols by reagent

Transfection Protocols (by reagent)

Transfection solutions for cancer cells

These transfection protocols for the most studied cancer cell lines and cancer types are designed to help you get the results you want the first time with minimal optimization. Find your cell line–specific protocol for breast, lung, prostate, or liver cancer. The new protocols provide you with a step-by-step guide to help ensure high transfection efficiency with low toxicity for your specific cancer cell line. 

Get your cancer cell protocol

Transfection solutions for stem cells

In addition to the protocol developed for the Lipofectamine Stem Transfection Reagent user guide we’ve created optimized stem cell–specific protocols that will help guide you to high efficiency with low toxicity in individual stem cell types (iPSC, ESC, NSC) and with your media of choice. The protocols cover growing cells, culture conditions, and passaging through to successful transfection results.


Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemFlex Medium


Transfection of pluripotent stem cells with Lipofectamine Stem reagent in mTeSR1 Medium on Geltrex matrix


Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in Essential 8 Medium


Transfection of neural stem cells with Lipofectamine Stem Transfection Reagent in StemPro medium

Application notes

Transfection application notes present research applications and proof-of-concept experiments involving Thermo Scientific, Invitrogen, and Gibco products from our cell biology portfolio.

If you're looking to learn about methods and techniques to achieve improved transfection, tested protocols, or descriptions of various techniques (and their trade-offs), you are likely to find such answers among these application notes.


Neurobiology transfection guide


Delivery solutions for immune cells


LentiVirus production


  Optimizing cardiomyocyte transfection with Lipofectamine MessengerMAX reagent


Improve genome editing outcomes In biologically relevant cell models


  Generation and transfection of induced pluripotent (iPSCs)


  Versatility and performance of Lipofectamine Stem Transfection Reagent in Stem Cells

Virtual lab

Virtual lab

The “Cell Culture Basics—Transfection” virtual training lab is an introduction to transfection covering many topics, including:

  • Overview of common transfection delivery methods
  • Conducting positive and negative controls
  • Performing a lipid-based transfection
  • Performing an electroporation transfection
  • Analyzing cells

Explore the lab

The Gibco cell culture basics—transfection lab is endorsed by The American Association for Cell Biology (ASCB). Gibco and ASCB are committed to cell biology education. As part of our partnership you will receive discounted membership to ASCB after completing the virtual lab.

ASCB logo

ASCB logo


Transfection handbook

This handbook outlines a collection of superior transfection and genome engineering products designed to work together to help answer research questions faster and with less effort.

Download now

Transfection protocol calculator

Let us take the stress and hassle out of scaling your experiment. Get your customized transfection protocol by entering just a few details.

Get your protocol


Transfection protocols

all protocols

Transfection Protocols (all)

protocols by cell type

Transfection Protocols (by cell type)

protocols by reagent

Transfection Protocols (by reagent)

Transfection solutions for cancer cells

These transfection protocols for the most studied cancer cell lines and cancer types are designed to help you get the results you want the first time with minimal optimization. Find your cell line–specific protocol for breast, lung, prostate, or liver cancer. The new protocols provide you with a step-by-step guide to help ensure high transfection efficiency with low toxicity for your specific cancer cell line. 

Get your cancer cell protocol

Transfection solutions for stem cells

In addition to the protocol developed for the Lipofectamine Stem Transfection Reagent user guide we’ve created optimized stem cell–specific protocols that will help guide you to high efficiency with low toxicity in individual stem cell types (iPSC, ESC, NSC) and with your media of choice. The protocols cover growing cells, culture conditions, and passaging through to successful transfection results.


Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemFlex Medium


Transfection of pluripotent stem cells with Lipofectamine Stem reagent in mTeSR1 Medium on Geltrex matrix


Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in Essential 8 Medium


Transfection of neural stem cells with Lipofectamine Stem Transfection Reagent in StemPro medium

Videos

Go to the Transfection Protocol Calculator


Addressing concerns of working with mRNA

Table of contents

Strategy

Methods

Delivery technologies


Resources