TaqPath qPCR and RT-qPCR Master Mixes
Enabling confidence and compliance for diagnostic development and testing
With superior quality, excellent performance, and regulatory compliance, Applied Biosystems™ TaqPath™ master mixes are designed to deliver confidence and performance for even your most demanding applications. With over 15 years of technology leadership in real-time PCR, we are committed to providing clinical laboratories with trusted, versatile, and innovative tools for molecular diagnostic development and testing.
TaqPath qPCR products
TaqPath qPCR master mixes have been validated to deliver exquisite lot-to-lot reproducibility for absolute Ct consistency and dynamic range across a wide variety of assays and applications. These general purpose reagents are manufactured in an ISO 13485 facility and include control measures, change control, document control, and purchasing controls. In addition, each batch is quality control–tested prior to release. TaqPath qPCR master mixes enable superior performance, improved reproducibility, and traceability.
Compatible with both standard and fast cycling modes, Applied Biosystems™ TaqPath™ qPCR Master Mixes have been engineered for enhanced stability, allowing your preassembled reactions to be left at room temperature for more than 24 hours without impacting performance. In addition, this product has been rigorously validated for duplex performance with both endogenous and exogenous controls, and each manufacturing lot is tested for duplex performance.
Through extensive benchmarking of this formulation against other leading suppliers’ standard and fast master mixes, TaqPath qPCR Master Mix has demonstrated the ability to deliver superior sensitivity, accuracy, dynamic range, and specificity.
Applied Biosystems™ TaqPath™ ProAmp™ master mixes are designed to be high-performing, high-throughput genotyping and copy number variation (CNV) master mixes that work even with inhibited samples. They have the ability to detect up to four targets in one reaction, providing high specificity and reproducible genotyping and copy number variation determination. They have a wide dynamic range compatible with multiplexing applications,** and they exhibit tolerance of inhibitors commonly found in clinical samples prepared from human or animal sources (buccal swabs, blood, and card punches). All TaqPath ProAmp master mixes also feature 72-hour pre-PCR benchtop stability for automated workflows.
Applied Biosystems™ TaqPath™ qPCR Master Mix, CG is a 2X formulation designed for gene expression and miRNA analysis, containing a thermostable fast DNA polymerase and dNTPs in an optimized buffer solution for maximum robustness and reproducibility.
TaqPath™ 1-Step Multiplex Master Mix is compatible with multiplexing reactions, allowing additional exogenous or endogenous controls or targets to be run simultaneously for quality control or increased efficiency. Both the Mustang Purple™ passive reference dye-containing mix and the NO ROX™ mix can be used in conjunction with TaqMan* probes with FAM™, VIC™, ABY™, and JUN™ dyes and QSY™ quenchers to provide detection of 4 targets in a single reaction.
TaqPath™ 1-Step RT-qPCR Master Mixes are 4X formulations designed for 1-step processing of RNA and DNA targets for pathogen detection, or for 1-step gene expression workflows. Versions with ROX passive reference dye, as well as versions designed specifically for multiplexing and formulated with either Mustang Purple passive reference dye or without ROX dye, are available.
Ease of use
These formulations combine thermostable MMLV reverse transcriptase, dNTPs, and fast DNA polymerase all in a single tube, so all you have to do is add your sample, target-specific primers, and probes.
Optimized to tolerate inhibitors commonly found in clinical samples and to enable superior sensitivity, specificity, and dynamic range with multiplexing compatibility, TaqPath 1-Step RT-qPCR Master Mixes are an ideal solution for your most challenging applications.
Each TaqPath qPCR and RT-qPCR master mix has been designed to deliver high reproducibility for absolute Ct consistency and dynamic range across a wide variety of assays and applications:
- Gene expression
- Copy number validation (CNV)
- MicroRNA (miRNA)
- Virus/pathogen detection
TaqPath qPCR master mixes enable superior performance, improved reproducibility, and traceability.
Q: With which cycling modes are TaqPath qPCR master mixes compatible?
A: TaqPath qPCR master mixes are compatible with both standard and fast cycling modes. Please refer to the product manual and your instrument guide for recommended cycling protocols.
Q: How stable are TaqPath qPCR master mixes?
A: TaqPath™ qPCR Master Mix, CG has been engineered for enhanced stability, allowing your preassembled reactions to be left at room temperature for more than 24 hours without impacting performance. In contrast, in the case of TaqPath™ 1-Step RT-qPCR Master Mixes, we recommend running reactions immediately after they are assembled.
Q: Are the TaqPath qPCR master mixes UDG/UNG-compatible?
A: TaqPath qPCR master mixes contain dUTP and UDG/UNG to mitigate potential risks of amplicon cross-contamination.
Q: How should the TaqPath qPCR master mixes be stored?
A: The recommended storage temperature for TaqPath qPCR Master Mix, CG is 4°C, and the recommended storage temperature for TaqPath 1-Step RT-qPCR Master Mixes is –20°C.
Q: How many reactions can I run using 1 package of the product?
A: It depends on the volume of your reactions. TaqPath qPCR Master Mix, CG is a 2X mix, so 5 mL is sufficient to run 500 standard (20 µL) reactions. In contrast, TaqPath 1-Step RT-qPCR Mixes are 4X mixes, so 5 mL is sufficient to run 1,000 reactions of 20 µL each.
General Purpose Reagent
For Laboratory Use.
* TaqMan is For Research Use Only. Not for use in diagnostic procedures.
** Dynamic range is a property of both the assay and template concentration in the sample, as well as the formulation of the master mix; thus, individual results may vary.
TaqMan is a trademark of Roche Molecular Systems, Inc., used under permission and license.