Coronavirus

Responsive, flexible, and sensitive portfolio of products to fit every need

Thermo Fisher Scientific is an industry-leading, worldwide, trusted partner with comprehensive, end-to-end research solutions for pathogen detection; scientific expertise; and the support capabilities critical to combating the current SARS-CoV-2 outbreak. We are committed to helping you rapidly address the outbreak with ready-to-use qPCR workflow research products, or the individual research components, referenced in CDC and WHO protocols.

For Research Use Only. Not for use in diagnostic procedures.

For the most up-to-date information on the current coronavirus outbreak, visit the WHO or CDC websites.

Now available: Singleplex kit for SARS-CoV-2 pathogen detection (For Research Use Only)

Our newly developed real-time PCR kit for SARS-CoV-2 pathogen detection research is now available to order. Our singleplex kit workflow was developed for use on the Applied Biosystems QuantStudio 5 or 7500 real time PCR systems. The kit utilizes Applied Biosystems TaqMan Assay technology, enabling specific, sensitive, and accurate results.

TaqMan 2019-nCoV Assay Kit v1 (singleplex)*
  • Singlexplex CoV Assay, for customers with low throughput samples
  • One coronavirus gene per well
  • FAM Dye
  • Kit includes four wet tubes containing assays, delivering 50 reactions
  • Control kit also available
  • Utilizes TaqPath 1-Step RT-qPCR Master Mix, CG 
Order now ›

* Although the singleplex kit has “2019-nCoV” in its product name, the kit is able to detect the SARS-CoV-2 pathogen. The kit was named and catalogued prior to recent WHO naming updates.

The TaqMan 2019-nCoV Assay Kit v1 (singleplex) features assays specifically targeted to all >100 complete genomes currently available at GISAID, and do not target any of the 2,116 complete genomes of other coronaviruses currently available at NCBI.

ProductComponentsQuantityCat. no.Documents
Singleplex solution
TaqMan 2019-nCoV Assay Kit v12019-nCoV Assay (orf1ab); 2019-nCoV Assay (spike (S) gene); 2019-nCoV Assay (nucleocapsid (N) gene); Rnase P (control)50 reactionsA47532Protocol
TaqMan 2019-nCoV Control Kit v1  2019-nCoV Control (covering orf1ab, spike (S) gene, nucleocapsid (N) gene, RNaseP)50 µL (1 x 104 copies/µL)A47533Protocol

End-to-end singleplex research solutions for SARS-CoV-2 pathogen detection by real-time PCR*

Figure 1. End-to-end research solution for SARS-CoV-2 pathogen detection by real-time PCR.

Recommended qPCR master mixes for use with singleplex kit for SARS-CoV-2 pathogen detection

Product

Quantity

Cat. no.

Use with:

TaqPath 1-Step RT-qPCR Master Mix, CG  (for use with TaqMan 2019-nCoV Assay Kit v1)5 x 1 mLA15299TaqMan 2019-nCoV Assay Kit v1 (singleplex)
10 mLA15300


Recommended qPCR systems for use with SARS-CoV-2 pathogen detection research

7500 Fast and 7500 real-time PCR systems

Applied Biosystems 7500 real-time PCR systems offer high performance, multicolor real-time PCR wherever your research takes you. Choose from our Fast (0.1 mL) and standard (0.2 mL) 7500 systems. All Applied Biosystems 7500 systems are designed to deliver the reliable performance and dye flexibility you need to generate data that you can trust.

QuantStudio 5 Real-Time PCR System (0.1- and 0.2-mL 96-well blocks, 384-well block)

The Applied Biosystems QuantStudio 5 Real-Time PCR System is ideal for all levels of users who need a high-performance instrument with features for maximum control of their experiments. Includes built-in security features, locked workflows, user account management, and options for web browser–based or desktop analysis.

Learn more about our real-time PCR systems ›

Thermo Fisher Scientific products referenced in publicly available real-time PCR protocols for SARS-CoV-2 pathogen detection

All of the below protocols can be found on the WHO website.

Organization/protocol Master mixInstrument
US Centers for Disease Control and Prevention (CDC)TaqPath 1-Step RT-qPCR Master Mix, CG
Charité, Berlin
Erasmus MC
Public Health England		SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
Hong Kong UniversityTaqMan Fast Virus 1-Step Master Mix

* For In Vitro Diagnostic Use.

Related products

Real-time PCR and 1-step RT-PCR master mixes

ProductDescriptionCat. no.
TaqMan Fast Virus 1-Step Master Mix
  • Highly concentrated (4X) master mix—add more sample for improved sensitivity
  • Increased tolerance to inhibitors for greater accuracy
  • One-tube master mix for improved efficiency and ease of use
  • Optimized for multiplexing (up to 3 targets) and with exogenous/endogenous internal controls
  • Fast cycling—provides results in ~60 min, half the time of standard cycling (also works with standard cycling)
4444434
TaqPath 1-Step RT-qPCR Master Mix, CGAll the advantages of TaqMan Fast Virus 1-Step Master Mix plus:
  • Includes UNG and dUTP for carryover contamination control
  • Enhanced quality control measures for even better lot-to-lot consistency
  • General purpose reagent
A15299  
SuperScript III Platinum One-Step qRT-PCR Kit
  • Delivers reliable, accurate target detection
  • Uses SuperScript III reverse transcriptase (RT) for higher temperature cDNA synthesis to overcome RNA secondary structure
  • Features Platinum Taq DNA Polymerase with hot-start technology for improved specificity
  • Verified performance on multiple real-time instrument platforms
11732020
SuperScript III One-Step RT-PCR system with Platinum Taq DNA Polymerase
  • Sensitive—routine detection down to 0.01 pg total RNA
  • Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
  • Specificity—uses SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene-specific primers
  • Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility
12574018

Viral nucleic acid isolation products

Isolate RNA from SARS-CoV-2 quickly and efficiently using the Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit and Thermo Scientific KingFisher purification instruments.

Isolate high-quality DNA and RNA with the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit. Features of this kit include:

  • Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems
  • Fast procedure allows for 96 samples to be processed in <30 minutes
  • Flexible protocol accommodates research sample volume inputs from 200 µL to 2 mL of blood, urine, swabs, lavage, and various transport media such as universal viral transport medium, and Eswab preservative fluid
  • Compatible with bronchoaveolar lavage and other respiratory research samples
  • Elution volumes ranging from 50 to 100 µL

Automate your DNA and RNA isolation with the KingFisher Flex system, a versatile benchtop instrument that processes 24 or 96 samples per run. Features of this system include:

  • High-throughput purification of 24 or 96 samples per run
  • Easy to install and run (ready to run in 10 minutes or less)
  • Choose from two plate formats for wide volume range (20–5,000 µL)
  • Download existing protocols from library, or easily customize protocols using Thermo Scientific BindIt Software


For Research Use Only. Not for use in diagnostic procedures.