MagMAX Viral and Pathogen Extraction Kits

MagMAX Viral and Pathogen kits and reagents use magnetic bead-based technology to purify high-quality DNA from a range of sample types. To help save time and increase reproducibility, combine with KingFisher instruments for automated DNA purification. 

With optimized automated protocols for our KingFisher system and updated reagents, the MagMAX Technology can be expanded into viral and infectious disease research.

  MagMAX Viral/Pathogen Nucleic Acid Isolation Kit
MagMAX Viral/Pathogen Nucleic Acid Isolation Kit
Order now
MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit
MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit
Order now
Who is it for? Research labs developing their own tests, that are on a budget but need high-throughput solutions for isolating viral nucleic acid (RNA and DNA) Research labs doing developing their own tests that require comprehensive pathogen lysis and recovery of nucleic acid (viral, gram positive bacteria, fungal, yeast)
Sample types Serum, plasma, urine, CSF, universal viral transport media, whole blood, and BAL Serum, plasma, urine, CSF, universal viral transport media, whole blood, BAL, swabs, and toe nail fungus
How our kits address pain points
  • Enables accurate and dependable results in a timely manner
  • Straightforward process with products that are affordable and enable a consistent, easy-to-use workflow
  • Enables accurate and dependable results in a timely manner
  • Straightforward process with products that are affordable and enable a consistent, easy-to-use workflow
  • Eliminates the bead-beating step, commonly required for lysis of gram positive bacteria, yeast, and fungal samples helping save time and hassle
Automation level Fully automation-ready solution
Elution volume 50 μL to 100 μL
Time per run 96 samples to be processed in <30 minutes 96 samples to be processed in <60 minutes
Cat. No. A42352 A42356

This MagMAX kit gives consistent, flexible/adjustable/scalable purification of viral nucleic acids, which eliminates need for carrier RNA and can be automated on KingFisher instruments (and fits within your budget).

Order nowDownload App Note

The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit is:

  • Scalable – There are just 2 protocols for low to high volume input range. Allowing for simple preparations of samples, run time and easy training.
  • Multi-sample – Like all MagMAX kits, the viral kit is compatible with multiple sample types
  • Recovery – Compatibility with low titer samples
  • No carrier required – Eliminate the need for a carrier, less interference w/ NGS and fewer steps required
  • Automation-ready – Optimized to work with KingFisher instruments

Viral Extraction Kit comparison – RNA virus

 Panel A 

Viral Extraction Kit comparison – RNA virus

 Panel B 

Viral Extraction Kit comparison – RNA virus

Figure 1. Viral Extraction Kit Comparison - RNA Virus. Nucleic acid was extracted using the MagMAX Viral/Pathogen Nucleic Isolation Kit and competitor kits from 5 different matrices spiked with inactivated viral particles Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate to determine performance between the kits. Panel A: (-) ssRNA Virus. Panel B: (+) ssRNA Virus

Viral Extraction Kit comparison – DNA virus

Viral Extraction Kit Comparison - DNA Virus

Figure 2: Viral Extraction Kit Comparison - DNA Virus. Nucleic acid was extracted using the MagMAX Viral/Pathogen Nucleic Isolation Kit and and competitor kits from 5 different matrices spiked with inactivated viral particles. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate to determine performance between the kits.


Frequently asked questions

Q: What is volume input range for the kit?

A: For the 96DW plate format: 200-400ul sample volume input (200ul for whole blood).
For the 24DW plate format: 500-2000ul sample volume input (1000ul for whole blood).

Q: What sample types are compatible with the kit?

A: Human biofluid samples (e.g. blood, serum, plasma, urine, CSF, lavage, saliva), samples stored in transport media (e.g. BD® Universal Viral Transport media), and bacteriophage culture.

Q: What is the lower limit for viral titer?

A: Nucleic acid has been successfully detected from as low as 50 viral copies in the starting sample.

Q: What types of pathogens are compatible?

A: (-)ssRNA virus, (+)ssRNA Virus, ssDNA virus, dsDNA virus, Gram negative bacteria.

Q: What minimum types of downstream analysis is the extracted nucleic acid compatible with?

A: qRT-PCR, qPCR, sequencing.

Q: How long is the protocol?

A: 30min from sample to extracted nucleic acid for up to 96 samples.

Q: Is the kit automation compatible?

A: Kit is compatible with all KingFisher instruments (Flex, Duo Prime, and Presto) and can also be used for manual processing in 96-well plate.

This MagMAX kit: provides an affordable and simple purification option for pathogen nucleic acids; eliminates lysis by bead-beating; and can be automated on KingFisher instruments.

Order nowDownload App Note

The MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit is:

  • Unique enzymatic solution – Eliminate the bead beating step by taking out the mechanical lysis with simplified enzymatic digestion
  • Recovery – Recover nucleic acid from a wide range of micro-organisms without additional processing steps
  • Scalable – Ability to process 200ul – 2ml using only 2 protocol options
  • Automation-ready – Optimized to work with KingFisher instruments

qRT-PCR Detection of RNA Virus at different inputs

 Panel A 

qRT-PCR Detection of RNA Virus at different inputs

 Panel B 

qRT-PCR Detection of RNA Virus at different inputs

Figure 3. qRT-PCR Detection of RNA Virus at different inputs. Nucleic acid was extracted using the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation kit from different matrices spiked with decreasing volumes of Viral Stock to demonstrate ability to recover material from variying titer viral RNA samples. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate with and without a pre-amplfication step. Panel A: (-) ssRNA Virus - Influenza A. Panel B: (+) ssRNA Virus - Rhinovirus.

qRT-PCR Detection of DNA Virus at different inputs

qRT-PCR Detection of DNA Virus at different inputs

Figure 4. qRT-PCR Detection of DNA Virus at different inputs. Nucleic acid was extracted using the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation kit from different matrices spiked with decreasing copies of DNA virus to demonstrate ability to recover material from varying titer viral DNA samples. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate with and without a pre-amplfication step.

qRT-PCR Detection of Bacteria at different inputs

 Panel A 

qRT-PCR Detection of Bacteria at different inputs

 Panel B 

qRT-PCR Detection of Bacteria at different inputs

 Panel C 

qRT-PCR Detection of Bacteria at different inputs

Figure 5: qRT-PCR Detection of Bacteria at different inputs. Nucleic acid was extracted using the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation kit from different matrices spiked with decreasing cfu inputs of 3 different bacteria species to demonstrate ability to recover material from varying sample titers. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate with and without a pre-amplfication step. Panel A: Gram Negative Bacteria - C. pneumoniae. Panel B: Gram Positive Bacteria - B. atrophaeus. Panel C: Gram Positive Bacteria - S. agalactiae.

qRT-PCR Detection of Fungi/Yeast at different inputs

qRT-PCR Detection of Fungi/Yeast at different inputs

Figure 6. qRT-PCR Detection of Fungi/Yeast at different inputs. Nucleic acid was extracted using the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation kit from different matrices spiked with decreasing cfu inputs of C. albicans to demonstrate ability to recover material from varying sample titers. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate with and without a pre-amplfication step.

 


Frequently asked questions

Q: What is volume input range for the kit?

A: For the 96DW plate format: 200-400ul sample volume input (200ul for whole blood).
For the 24DW plate format: 500-2000ul sample volume input (1000ul for whole blood).

Q: What sample types are compatible with the kit?

A: Human biofluid samples (e.g. blood, serum, plasma, urine, CSF, lavage, saliva) and samples stored in transport media (e.g. BD® Universal Viral Transport media).

Q: What is the detection limit for microbes?

A: Nucleic acid has been successfully detected from as low as 50 viral copies and 1000cfu of bacteria and yeast/fungi in the starting sample.

Q: What types of pathogens have been tested?

A: Multiple targets each of: (-)ssRNA virus, (+)ssRNA Virus, ssDNA virus, dsDNA virus, Gram negative bacteria, gram positive bacteria, yeast, yeast-like fungi, and fungi.

Q: What minimum types of downstream analysis is the extracted nucleic acid compatible with?

A: qRT-PCR, qPCR, sequencing.

Q: How long is the protocol?

A: <60min from sample to extracted nucleic acid for up to 96 samples.

Q: Is the kit automation compatible?

A: Kit is compatible with all KingFisher instruments (Flex, Duo Prime, and Presto) and can also be used for manual processing in 96-well plate.

  MagMAX Viral/Pathogen Nucleic Acid Isolation Kit
MagMAX Viral/Pathogen Nucleic Acid Isolation Kit
Order now
MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit
MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit
Order now
Who is it for? Research labs developing their own tests, that are on a budget but need high-throughput solutions for isolating viral nucleic acid (RNA and DNA) Research labs doing developing their own tests that require comprehensive pathogen lysis and recovery of nucleic acid (viral, gram positive bacteria, fungal, yeast)
Sample types Serum, plasma, urine, CSF, universal viral transport media, whole blood, and BAL Serum, plasma, urine, CSF, universal viral transport media, whole blood, BAL, swabs, and toe nail fungus
How our kits address pain points
  • Enables accurate and dependable results in a timely manner
  • Straightforward process with products that are affordable and enable a consistent, easy-to-use workflow
  • Enables accurate and dependable results in a timely manner
  • Straightforward process with products that are affordable and enable a consistent, easy-to-use workflow
  • Eliminates the bead-beating step, commonly required for lysis of gram positive bacteria, yeast, and fungal samples helping save time and hassle
Automation level Fully automation-ready solution
Elution volume 50 μL to 100 μL
Time per run 96 samples to be processed in <30 minutes 96 samples to be processed in <60 minutes
Cat. No. A42352 A42356

This MagMAX kit gives consistent, flexible/adjustable/scalable purification of viral nucleic acids, which eliminates need for carrier RNA and can be automated on KingFisher instruments (and fits within your budget).

Order nowDownload App Note

The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit is:

  • Scalable – There are just 2 protocols for low to high volume input range. Allowing for simple preparations of samples, run time and easy training.
  • Multi-sample – Like all MagMAX kits, the viral kit is compatible with multiple sample types
  • Recovery – Compatibility with low titer samples
  • No carrier required – Eliminate the need for a carrier, less interference w/ NGS and fewer steps required
  • Automation-ready – Optimized to work with KingFisher instruments

Viral Extraction Kit comparison – RNA virus

 Panel A 

Viral Extraction Kit comparison – RNA virus

 Panel B 

Viral Extraction Kit comparison – RNA virus

Figure 1. Viral Extraction Kit Comparison - RNA Virus. Nucleic acid was extracted using the MagMAX Viral/Pathogen Nucleic Isolation Kit and competitor kits from 5 different matrices spiked with inactivated viral particles Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate to determine performance between the kits. Panel A: (-) ssRNA Virus. Panel B: (+) ssRNA Virus

Viral Extraction Kit comparison – DNA virus

Viral Extraction Kit Comparison - DNA Virus

Figure 2: Viral Extraction Kit Comparison - DNA Virus. Nucleic acid was extracted using the MagMAX Viral/Pathogen Nucleic Isolation Kit and and competitor kits from 5 different matrices spiked with inactivated viral particles. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate to determine performance between the kits.


Frequently asked questions

Q: What is volume input range for the kit?

A: For the 96DW plate format: 200-400ul sample volume input (200ul for whole blood).
For the 24DW plate format: 500-2000ul sample volume input (1000ul for whole blood).

Q: What sample types are compatible with the kit?

A: Human biofluid samples (e.g. blood, serum, plasma, urine, CSF, lavage, saliva), samples stored in transport media (e.g. BD® Universal Viral Transport media), and bacteriophage culture.

Q: What is the lower limit for viral titer?

A: Nucleic acid has been successfully detected from as low as 50 viral copies in the starting sample.

Q: What types of pathogens are compatible?

A: (-)ssRNA virus, (+)ssRNA Virus, ssDNA virus, dsDNA virus, Gram negative bacteria.

Q: What minimum types of downstream analysis is the extracted nucleic acid compatible with?

A: qRT-PCR, qPCR, sequencing.

Q: How long is the protocol?

A: 30min from sample to extracted nucleic acid for up to 96 samples.

Q: Is the kit automation compatible?

A: Kit is compatible with all KingFisher instruments (Flex, Duo Prime, and Presto) and can also be used for manual processing in 96-well plate.

This MagMAX kit: provides an affordable and simple purification option for pathogen nucleic acids; eliminates lysis by bead-beating; and can be automated on KingFisher instruments.

Order nowDownload App Note

The MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit is:

  • Unique enzymatic solution – Eliminate the bead beating step by taking out the mechanical lysis with simplified enzymatic digestion
  • Recovery – Recover nucleic acid from a wide range of micro-organisms without additional processing steps
  • Scalable – Ability to process 200ul – 2ml using only 2 protocol options
  • Automation-ready – Optimized to work with KingFisher instruments

qRT-PCR Detection of RNA Virus at different inputs

 Panel A 

qRT-PCR Detection of RNA Virus at different inputs

 Panel B 

qRT-PCR Detection of RNA Virus at different inputs

Figure 3. qRT-PCR Detection of RNA Virus at different inputs. Nucleic acid was extracted using the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation kit from different matrices spiked with decreasing volumes of Viral Stock to demonstrate ability to recover material from variying titer viral RNA samples. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate with and without a pre-amplfication step. Panel A: (-) ssRNA Virus - Influenza A. Panel B: (+) ssRNA Virus - Rhinovirus.

qRT-PCR Detection of DNA Virus at different inputs

qRT-PCR Detection of DNA Virus at different inputs

Figure 4. qRT-PCR Detection of DNA Virus at different inputs. Nucleic acid was extracted using the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation kit from different matrices spiked with decreasing copies of DNA virus to demonstrate ability to recover material from varying titer viral DNA samples. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate with and without a pre-amplfication step.

qRT-PCR Detection of Bacteria at different inputs

 Panel A 

qRT-PCR Detection of Bacteria at different inputs

 Panel B 

qRT-PCR Detection of Bacteria at different inputs

 Panel C 

qRT-PCR Detection of Bacteria at different inputs

Figure 5: qRT-PCR Detection of Bacteria at different inputs. Nucleic acid was extracted using the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation kit from different matrices spiked with decreasing cfu inputs of 3 different bacteria species to demonstrate ability to recover material from varying sample titers. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate with and without a pre-amplfication step. Panel A: Gram Negative Bacteria - C. pneumoniae. Panel B: Gram Positive Bacteria - B. atrophaeus. Panel C: Gram Positive Bacteria - S. agalactiae.

qRT-PCR Detection of Fungi/Yeast at different inputs

qRT-PCR Detection of Fungi/Yeast at different inputs

Figure 6. qRT-PCR Detection of Fungi/Yeast at different inputs. Nucleic acid was extracted using the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation kit from different matrices spiked with decreasing cfu inputs of C. albicans to demonstrate ability to recover material from varying sample titers. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate with and without a pre-amplfication step.

 


Frequently asked questions

Q: What is volume input range for the kit?

A: For the 96DW plate format: 200-400ul sample volume input (200ul for whole blood).
For the 24DW plate format: 500-2000ul sample volume input (1000ul for whole blood).

Q: What sample types are compatible with the kit?

A: Human biofluid samples (e.g. blood, serum, plasma, urine, CSF, lavage, saliva) and samples stored in transport media (e.g. BD® Universal Viral Transport media).

Q: What is the detection limit for microbes?

A: Nucleic acid has been successfully detected from as low as 50 viral copies and 1000cfu of bacteria and yeast/fungi in the starting sample.

Q: What types of pathogens have been tested?

A: Multiple targets each of: (-)ssRNA virus, (+)ssRNA Virus, ssDNA virus, dsDNA virus, Gram negative bacteria, gram positive bacteria, yeast, yeast-like fungi, and fungi.

Q: What minimum types of downstream analysis is the extracted nucleic acid compatible with?

A: qRT-PCR, qPCR, sequencing.

Q: How long is the protocol?

A: <60min from sample to extracted nucleic acid for up to 96 samples.

Q: Is the kit automation compatible?

A: Kit is compatible with all KingFisher instruments (Flex, Duo Prime, and Presto) and can also be used for manual processing in 96-well plate.

KingFisher Instruments

Just like all Applied Biosystems MagMAX kits, viral and pathogen kits are optimised for KingFisher instruments:

  • The KingFisher system can bind, wash, and elute for you
  • Scalable from 200 μL–2 mL with only two protocols

Find out more