MagMAX Viral and Pathogen Extraction Kits

MagMAX Viral/Pathogen kits and reagents use magnetic bead-based technology to purify high-quality nucleic acid from a range of research sample types both manually and through automation. To help save time and increase reproducibility, combine with KingFisher instruments for automated purification. Our kits are recommended for viral nucleic acid isolation for SARS Cov-2.

The following products are recommended for viral nucleic acid isolation for SARS-Cov-2. To learn about the differences between the kits, please see Frequently Asked Questions below.

 Brochure: SARS-CoV-2 support and solutions KingFisher instruments and MagMAX isolation kits
 Application note: MagMAX Viral/Pathogen kit protocol changes to enable increased SARS-CoV-2 testing throughput

Select between MagMAX kits for coronavirus testing

  MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I)
MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I)

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MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (MVP II)
MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (MVP II)

Order now
Who is it for? These MagMAX Viral/Pathogen Nucleic Acid Isolation Kits are designed for use by research labs and facilities currently testing for SARS-CoV-2, or labs developing their own tests
Sample types Universal viral transport media (VTM), bronchoalveolar lavage (BAL) and other respiratory research samples, serum, plasma, urine, CSF, whole blood, and BAL
Automation level Automation-ready protocols designed for Thermo Scientific KingFisher Flex and KingFisher Duo Prime systems
Sample input volume 200 µL of VTM sample recommended
Elution volume 50 μL 
Time per run 96 samples can be processed in <40 minutes
Protocol update
  • You can now use a sample input volume of 200 μL (vs. the standard 400 μL) with a reduced (halved) amount of extraction reagents
  • This means that you can now get up to 2,000 reactions from your MVP I (A48310) or MVP II (A48383) products. Additionally, the smaller pack size of the MVP I kit (A42352) can support up to 200 reactions
What is the difference between the two kits?

Binding Beads: In order to support the unprecedented demand for RNA extraction products for COVID-19 testing. There has been a raw material change in the bead component contained in the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit. The MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit contains these beads. The chemistry driving the RNA to the beads is identical, and the nucleic acid binding capacity of each bead type is sufficient to provide similar results between the two products.

Bulk reagent format: The MagMAX Viral/Pathogen II kit is only available in bulk prep format with 1,000 or 2,000 reactions.

Cat. No. A48310 (up to 2,000 preps/kit)
This kit contains the following SKUS: A42359, A42360, A42362, A42363, A42364
A48383 (up to 2,000 preps/kit)
A42352 (up to 200 preps/kit)

Protocols

The following protocols can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVP II) for SARS-CoV-2 testing, which supports sample input volume reduction of 400 μL to 200 μL, and reduction from 3 wash steps to 2 wash steps. Protocols can be downloaded for the KingFisher Flex 96 Deep-well head (5400630).

Protocol type Standard wash (3 wash steps) Reduced wash (2 wash steps)
200 μL script MVP_Flex_200ul.bdz MVP_2Wash_200_Flex.bdz
400 μL script MVP_Flex_400ul.bdz MVP_2Wash_400_Flex.bdz

Want to isolate without an instrument? See our manual protocol/user guide for use with magnetic stands

The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit is:

  • Scalable – There are just 2 protocols for low to high volume input range. Allowing for simple preparations of samples, run time and easy training
  • Multi-sample – Like all MagMAX kits, the viral kit is compatible with multiple sample types
  • Recovery – Compatibility with low titer samples
  • No carrier required – Eliminate the need for a carrier, less interference w/ NGS and fewer steps required
  • Automation-ready – Optimized to work with KingFisher instruments

Download App Note


Viral Extraction Kit comparison – RNA virus

 Panel A 

Viral Extraction Kit comparison – RNA virus

 Panel B 

Viral Extraction Kit comparison – RNA virus

Figure 1. Viral Extraction Kit Comparison - RNA Virus. Nucleic acid was extracted using the MagMAX Viral/Pathogen Nucleic Isolation Kit and competitor kits from 5 different matrices spiked with inactivated viral particles Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate to determine performance between the kits. Panel A: (-) ssRNA Virus. Panel B: (+) ssRNA Virus

Viral Extraction Kit comparison – DNA virus

Viral Extraction Kit Comparison - DNA Virus

Figure 2: Viral Extraction Kit Comparison - DNA Virus. Nucleic acid was extracted using the MagMAX Viral/Pathogen Nucleic Isolation Kit and and competitor kits from 5 different matrices spiked with inactivated viral particles. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate to determine performance between the kits.

Q. What is the difference between the original MVP I kits (A42352, A48310) and the MVP II kit (A48383)?

A. There are two key differences between the MVP I and MVP II products:

Binding Beads: A proprietary raw material change in the bead component is different. The chemistry driving the RNA to the beads is identical and the nucleic acid binding capacity provides similar/equivalent results between the two products. Bulk reagent format: The second difference is that the MVP II (A48383) product is only available in the bulk format. There is no 100-reaction option for the MVP II kit.

 

Q. Can both kits be used without an instrument?

A. Yes, we have a manual ready protocol and user guide for use with a magnetic stand.

 

Q. Are other product contents and workflow the same for MVP I kits (A42352, A48310) and the MVP II kit (A48383)?

A. Yes, the product contents (Binding Solution, Wash Buffer, Proteinase K, and Elution Solution formulations) are the same in both kits.

Yes, the overall workflow, in terms of number of steps and volume usage, remains the same; if you have been using the MVP I (A42353, A48310) kit you should not have to change your protocol.

IMPORTANT: The protocol for both the MVP I and MVP II products has been updated to include a separate lower input option: You can now use a sample input volume of 200 μL (vs. the standard 400 μL) with a reduced (halved) amount of extraction reagents.  What does this mean? It means that you can now get 2,000 preps from your MVP I (A48310) or MVP II (A48383) products. This also means that the smaller pack size of the MVP I kit (A42352) can support 200 preps.

 

Q. Can I use the same KingFisher Flex script for both the MVP I and MVP II kit?

A. Yes, there will be a new script for the 200 μL sample input protocol and both kit versions can be used with it.

 

Q. Can I use the same samples with the MVP II kit as I am with the MVP I kit?

A. Yes, all the same samples are compatible.

 

Q. Is MVP II compatible with the TaqPath COVID-19 Combo Kit?

A. Yes. You can use the MVP II (A48383) product just as you would use the MVP I (A42352, A48310) product with the TaqPath COVID-19 Combo Kit. When using the 200 μL sample input protocol, please note that the eluate input into the PCR will be changed to 10 uL (5uL eluate input when using 400 μL).

 

Q.  Is the new MVP II kit replacing the current MVP I kit?

A. No, the MVP II is NOT replacing the existing MagMAX Viral/Pathogen Kit (Cat. Nos. A423352, A48310).  The intent of this product to expand the number of samples that can be processed with our MagMAX reagents.

 

Q.  Are both the MVP I and MVP II products manufactured under the same ISO 13485 quality standards?

A. Yes, they are both manufactured under the same ISO 13485 standards which utilizes a high set of quality control measures.

 

Q.  Why would I order the MVP I kit vs the MVP II kit?

A: Availability

 

Q:  Is there a difference in price?

A: No, the bulk reagents for both kits are the same price.

 

Q: What is volume input range for the kit?

A: For the 96DW plate format: 200-400 μL sample volume input (200 μL for whole blood).
For the 24DW plate format: 500-2000 μL sample volume input (1000 μL for whole blood).

 

Q: What sample types are compatible with the kit?

A: Human biofluid samples (e.g. blood, serum, plasma, urine, CSF, lavage, saliva), samples stored in transport media (e.g. BD® Universal Viral Transport media), and bacteriophage culture.

 

Q: What is the lower limit for viral titer?

A: Nucleic acid has been successfully detected from as low as 50 viral copies in the starting sample.

 

Q: What types of pathogens are compatible?

A: (-)ssRNA virus, (+)ssRNA Virus, ssDNA virus, dsDNA virus, Gram negative bacteria.

 

Q: What minimum types of downstream analysis is the extracted nucleic acid compatible with?

A: qRT-PCR, qPCR, sequencing.

 

Q: How long is the protocol?

A: 30min from sample to extracted nucleic acid for up to 96 samples.

 

Q: Is the kit automation compatible?

A: Kit is compatible with all KingFisher instruments (Flex, Duo Prime, and Presto) and can also be used for manual processing in 96-well plate.

Related products for plate setup

To help save time and increase reproducibility with the MagMAX Viral/Pathogen Nucleic Acid Isolation kits, combine with E1-ClipTip Equalizer electronic pipettes and Multidrop Combi instruments.

E1-ClipTip Equalizer Pipettes

E1-ClipTip Equalizer Pipettes

Adjustable spacing, Bluetooth enabled electronic pipette. Learn more

Multidrop Combi Reagent Dispenser

Multidrop Combi Reagent Dispenser

Automated reagent dispenser. Learn more

Select between MagMAX kits for coronavirus testing

  MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I)
MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I)

Order now
MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (MVP II)
MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (MVP II)

Order now
Who is it for? These MagMAX Viral/Pathogen Nucleic Acid Isolation Kits are designed for use by research labs and facilities currently testing for SARS-CoV-2, or labs developing their own tests
Sample types Universal viral transport media (VTM), bronchoalveolar lavage (BAL) and other respiratory research samples, serum, plasma, urine, CSF, whole blood, and BAL
Automation level Automation-ready protocols designed for Thermo Scientific KingFisher Flex and KingFisher Duo Prime systems
Sample input volume 200 µL of VTM sample recommended
Elution volume 50 μL 
Time per run 96 samples can be processed in <40 minutes
Protocol update
  • You can now use a sample input volume of 200 μL (vs. the standard 400 μL) with a reduced (halved) amount of extraction reagents
  • This means that you can now get up to 2,000 reactions from your MVP I (A48310) or MVP II (A48383) products. Additionally, the smaller pack size of the MVP I kit (A42352) can support up to 200 reactions
What is the difference between the two kits?

Binding Beads: In order to support the unprecedented demand for RNA extraction products for COVID-19 testing. There has been a raw material change in the bead component contained in the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit. The MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit contains these beads. The chemistry driving the RNA to the beads is identical, and the nucleic acid binding capacity of each bead type is sufficient to provide similar results between the two products.

Bulk reagent format: The MagMAX Viral/Pathogen II kit is only available in bulk prep format with 1,000 or 2,000 reactions.

Cat. No. A48310 (up to 2,000 preps/kit)
This kit contains the following SKUS: A42359, A42360, A42362, A42363, A42364
A48383 (up to 2,000 preps/kit)
A42352 (up to 200 preps/kit)

Protocols

The following protocols can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVP II) for SARS-CoV-2 testing, which supports sample input volume reduction of 400 μL to 200 μL, and reduction from 3 wash steps to 2 wash steps. Protocols can be downloaded for the KingFisher Flex 96 Deep-well head (5400630).

Protocol type Standard wash (3 wash steps) Reduced wash (2 wash steps)
200 μL script MVP_Flex_200ul.bdz MVP_2Wash_200_Flex.bdz
400 μL script MVP_Flex_400ul.bdz MVP_2Wash_400_Flex.bdz

Want to isolate without an instrument? See our manual protocol/user guide for use with magnetic stands

The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit is:

  • Scalable – There are just 2 protocols for low to high volume input range. Allowing for simple preparations of samples, run time and easy training
  • Multi-sample – Like all MagMAX kits, the viral kit is compatible with multiple sample types
  • Recovery – Compatibility with low titer samples
  • No carrier required – Eliminate the need for a carrier, less interference w/ NGS and fewer steps required
  • Automation-ready – Optimized to work with KingFisher instruments

Download App Note


Viral Extraction Kit comparison – RNA virus

 Panel A 

Viral Extraction Kit comparison – RNA virus

 Panel B 

Viral Extraction Kit comparison – RNA virus

Figure 1. Viral Extraction Kit Comparison - RNA Virus. Nucleic acid was extracted using the MagMAX Viral/Pathogen Nucleic Isolation Kit and competitor kits from 5 different matrices spiked with inactivated viral particles Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate to determine performance between the kits. Panel A: (-) ssRNA Virus. Panel B: (+) ssRNA Virus

Viral Extraction Kit comparison – DNA virus

Viral Extraction Kit Comparison - DNA Virus

Figure 2: Viral Extraction Kit Comparison - DNA Virus. Nucleic acid was extracted using the MagMAX Viral/Pathogen Nucleic Isolation Kit and and competitor kits from 5 different matrices spiked with inactivated viral particles. Once extracted, one-step qRT-PCR was performed on 1ul of each sample in duplicate to determine performance between the kits.

Q. What is the difference between the original MVP I kits (A42352, A48310) and the MVP II kit (A48383)?

A. There are two key differences between the MVP I and MVP II products:

Binding Beads: A proprietary raw material change in the bead component is different. The chemistry driving the RNA to the beads is identical and the nucleic acid binding capacity provides similar/equivalent results between the two products. Bulk reagent format: The second difference is that the MVP II (A48383) product is only available in the bulk format. There is no 100-reaction option for the MVP II kit.

 

Q. Can both kits be used without an instrument?

A. Yes, we have a manual ready protocol and user guide for use with a magnetic stand.

 

Q. Are other product contents and workflow the same for MVP I kits (A42352, A48310) and the MVP II kit (A48383)?

A. Yes, the product contents (Binding Solution, Wash Buffer, Proteinase K, and Elution Solution formulations) are the same in both kits.

Yes, the overall workflow, in terms of number of steps and volume usage, remains the same; if you have been using the MVP I (A42353, A48310) kit you should not have to change your protocol.

IMPORTANT: The protocol for both the MVP I and MVP II products has been updated to include a separate lower input option: You can now use a sample input volume of 200 μL (vs. the standard 400 μL) with a reduced (halved) amount of extraction reagents.  What does this mean? It means that you can now get 2,000 preps from your MVP I (A48310) or MVP II (A48383) products. This also means that the smaller pack size of the MVP I kit (A42352) can support 200 preps.

 

Q. Can I use the same KingFisher Flex script for both the MVP I and MVP II kit?

A. Yes, there will be a new script for the 200 μL sample input protocol and both kit versions can be used with it.

 

Q. Can I use the same samples with the MVP II kit as I am with the MVP I kit?

A. Yes, all the same samples are compatible.

 

Q. Is MVP II compatible with the TaqPath COVID-19 Combo Kit?

A. Yes. You can use the MVP II (A48383) product just as you would use the MVP I (A42352, A48310) product with the TaqPath COVID-19 Combo Kit. When using the 200 μL sample input protocol, please note that the eluate input into the PCR will be changed to 10 uL (5uL eluate input when using 400 μL).

 

Q.  Is the new MVP II kit replacing the current MVP I kit?

A. No, the MVP II is NOT replacing the existing MagMAX Viral/Pathogen Kit (Cat. Nos. A423352, A48310).  The intent of this product to expand the number of samples that can be processed with our MagMAX reagents.

 

Q.  Are both the MVP I and MVP II products manufactured under the same ISO 13485 quality standards?

A. Yes, they are both manufactured under the same ISO 13485 standards which utilizes a high set of quality control measures.

 

Q.  Why would I order the MVP I kit vs the MVP II kit?

A: Availability

 

Q:  Is there a difference in price?

A: No, the bulk reagents for both kits are the same price.

 

Q: What is volume input range for the kit?

A: For the 96DW plate format: 200-400 μL sample volume input (200 μL for whole blood).
For the 24DW plate format: 500-2000 μL sample volume input (1000 μL for whole blood).

 

Q: What sample types are compatible with the kit?

A: Human biofluid samples (e.g. blood, serum, plasma, urine, CSF, lavage, saliva), samples stored in transport media (e.g. BD® Universal Viral Transport media), and bacteriophage culture.

 

Q: What is the lower limit for viral titer?

A: Nucleic acid has been successfully detected from as low as 50 viral copies in the starting sample.

 

Q: What types of pathogens are compatible?

A: (-)ssRNA virus, (+)ssRNA Virus, ssDNA virus, dsDNA virus, Gram negative bacteria.

 

Q: What minimum types of downstream analysis is the extracted nucleic acid compatible with?

A: qRT-PCR, qPCR, sequencing.

 

Q: How long is the protocol?

A: 30min from sample to extracted nucleic acid for up to 96 samples.

 

Q: Is the kit automation compatible?

A: Kit is compatible with all KingFisher instruments (Flex, Duo Prime, and Presto) and can also be used for manual processing in 96-well plate.

Related products for plate setup

To help save time and increase reproducibility with the MagMAX Viral/Pathogen Nucleic Acid Isolation kits, combine with E1-ClipTip Equalizer electronic pipettes and Multidrop Combi instruments.

E1-ClipTip Equalizer Pipettes

E1-ClipTip Equalizer Pipettes

Adjustable spacing, Bluetooth enabled electronic pipette. Learn more

Multidrop Combi Reagent Dispenser

Multidrop Combi Reagent Dispenser

Automated reagent dispenser. Learn more

KingFisher Instruments

Just like all Applied Biosystems MagMAX kits, viral and pathogen kits are optimized for KingFisher instruments:

  • The KingFisher system can bind, wash, and elute for you
  • Scalable from 200 μL–2 mL with only two protocols

Find out more