Vanquish Duo UHPLC Systems

Double your throughput or deepen your sample knowledge using the Thermo Scientific Vanquish Duo UHPLC Systems, featuring a dual split sampler and two pumps, either as separate modules or combined in the dual pump. The Vanquish Duo UHPLC system has the same footprint as a single instrument, but contains two flow paths allowing you to run the same method on both flow paths or run two different methods simultaneously on the same instrument, improving your lab productivity, sample knowledge, and overall return on investment.


Doubled productivity without sacrificing bench space

The Vanquish Duo UHPLC system provides two independent flow paths connected to two individual detectors. This unique system lets you run two analyses, either identical or not, on a single system to double throughput, accelerate method development, and improve sample characterization.

Complementary analysis

Analyze the same set of samples using multiple methods or column chemistries while reducing sample preparation time and costs and maximizing sample information for faster biopharmaceutical characterization, or simultaneous API quantification and impurity analysis for pharmaceuticals.

Innovative dual split sampler and dual pump

The Thermo Scientific Vanquish Dual Split Sampler includes two injection devices in a single housing, for two individually controlled channels. Additionally, the Thermo Scientific Vanquish Dual Pump has two built-in independent ternary pumps saving purchasing costs and reducing system height.

Versatility in analyte detection

A variety of detection options ensure you can collect the most information from your separation. All detector options offer excellent sensitivity, including diode array, fluorescence, or label-free detection of non-chromophoric substances via charged aerosol detection.

Biocompatible, easy and reliable tool-free connections

The fluidic pathway of the Vanquish Duo UHPLC system is fully biocompatible and fitted with Thermo Scientific Viper Fingertight Fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.

CDS software

The Vanquish Duo systems seamlessly integrate with our Thermo Scientific Chromeleon Chromatography Data System (CDS) to deliver superior compliance tools, networking capabilities, automation and data processing.

What is Vanquish Duo?

Our Vanquish Duo UHPLC systems allow you to run two of the same or two different assays simultaneously.


The diagram below illustrates the time savings achieved when running two different assays on the Vanquish Duo simultaneously, in comparison to the time required to run the same two assays on a traditional single-channel HPLC system.


System specifications

The table below provides an overview of the Vanquish Duo UHPLC systems specifications for a standard setup. Specifications may differ based on the specific system components selected. Your specifications may differ based on the components of your system. Find the specification sheets for the system components in the Component specifications, below.

Category Vanquish Duo UHPLC Systems
System pressure, maximum 1000 or 1500 bar, depends on configuration
Pump options (default gradient delay volume)

Binary (35 or 200 µL)

Quaternary (679 µL) 

Dual (679 µL)  

Delay volume dependent on pump(s) selected

Biocompatible Yes
pH range 2–12, chloride concentration up to 1 mol/L
System design Modular
Touchscreen user interface display compatible (optional) Yes, depends on system configuration
Solvent monitoring system compatible (optional) Yes, depends on system configuration
Customizable gradient delay volume to simplify method transfer Yes, with metering device
SmartInject technology for maximum injection precision and improved column lifetime


Injection volume range Standard: 0.01-25 µL 
Optional: 0.01-100 µL

Sample cooling

Yes, 4-40°C

Sample capacity (standard) 
Based on 12 mm OD vials (≤1.5 mL) for comparison

4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available 
+ capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel
Sampler extension available (optional)

Yes, with Vanquish Charger

Column compartment thermostatting

Yes, 5-120°C 
Still air and forced air

Column compartment pre-heater Active and passive 
Column compartment post-column cooler Yes
Compatible detectors

Vanquish Charged Aerosol Detector 

Vanquish Diode Array Detector  

Vanquish Fluorescence Detector 

Vanquish Multiple Wavelength Detector 

Vanquish Variable Wavelength Detector

Refractive Index Detector 

Mass Spectrometer

Component specifications

Vanquish Duo UHPLC systems are made up of the following components with detail available below.

  Vanquish Horizon Duo UHPLC System Vanquish Flex Duo UHPLC System



Download Specification Sheet

Vanquish Binary Pump H Vanquish Binary Pump F 
Vanquish Quaternary Pump F 
Vanquish Dual Pump F



Download Specification Sheet

Vanquish Dual Split Sampler HT Vanquish Dual Split Sampler FT

Extended sample capacity (optional)


Download Specification Sheet

Vanquish Charger  (optional)

Column compartment


Download Specification Sheet

Vanquish Column Compartment H

Virtually zero dead volume system connections


Download Specification Sheet

Viper UHPLC Fittings Kits



2 Detectors

Visit our HPLC and UHPLC Detectors page

Contact us to get a quote or discuss an HPLC/UHPLC solution

For Research Use Only. Not for use in diagnostic procedures.