Lamin A and C are A-type lamins that arise from alternative splicing of the LMNA gene. As part of the lamin class of nuclear intermediate filament proteins, lamin A and C are major structural components of the matrix on the inner surface of the nuclear envelope. During mitosis, lamin proteins are phosphorylated, resulting in the reversible disassembly of the lamina matrix. Lamins are also involved in chromatin structure, gene expression, nuclear stability, and apoptosis. Because they are ubiquitously expressed nuclear proteins, lamin A and C are useful as loading controls.
Mutations in lamin A and C have been linked to several human diseases including Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy, limb-girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Invitrogen lamin AC antibodies are validated for use in various applications. To see all the lamin AC antibody offerings please click the link below.
A variety of quality Invitrogen lamin A/C antibodies are available for your research, and most have been extensively validated for western blotting and immunostaining controls.
Immunofluorescent analysis of Lamin A/C shows staining in U251 Cells. Lamin A/C (green), F-actin staining with phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C (Cat. No. MA3-1000) at a dilution of 1:200 overnight at 4oC, washed with PBS and incubated with a DyLight 488-conjugated secondary antibody (Cat. No. 35552 for GAR; Cat. No. 35503 for GAM). Images were taken at 60x magnification.
Immunohistochemistry analysis of Lamin C on formalin-fixed paraffin embedded skin tissue. Specific detection was accomplished using lamin C polyclonal antibody (Cat. No. PA1-5827) at a dilution of 1:100.