Zinc Indicators

Zinc concentrations in the 1–100 nM range are routinely measured using fluorescent indicators nominally designed for Ca2+ detection, but higher Zn2+ concentrations are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists.

We’ve developed a number of Molecular Probes ion indicators to track zinc with intense fluorescent signals and a range of wavelength options.

Select zinc indicators ›

Indicators for Zinc

  • Optimized for higher Zn2+ concentrations
  • Compatible with multiple analysis platforms

FluoZin dyes are unique indicators designed for detection of Zn2+ in the 0.1–100 µM range with minimal interfering Ca2+ sensitivity. They provide bright signals for assaying exocytotic release of Zn2+ from pancreatic β-cells after stimulation with glucose or potassium. Zn2+ concentrations can be measured using imaging platforms, cell-based microplate assays, or flow cytometry.

BioProbes
Human vascular smooth muscle cell loaded with FluoZin indicator and stained with Hoechst 3342.

Zinc Indicators Selection Guide

Used for spatial and temporal resolution of Zn2+ concentrations.

  FluoZin-1, Tripotassium Salt FluoZin-1, AM FluoZin-3, Tetrapotassium Salt FluoZin-3, AM
Readout Responds to Zn2+ concentrations in synaptic vesicles with increasing fluorescence Responds to saturating levels of Zn2+ with 50-fold increase in fluorescence emission
Range 0–100 µM range with minimal interfering Ca2+ sensitivity Low nM to µM range with minimal interfering Ca2+ sensitivity
Fluorophore FluoZin-1 FluoZin-3
Standard filter set FITC
Ex/Em (nm) 495/515 494/516
Bibliography Citations Citations
Cell permeant or impermeant? Impermeant Permeant Impermeant Permeant
Platforms Imaging Imaging Imaging Imaging
Usage notes Load by microinjection, patch pipette, or pinocytic loading agent Load in cell media Load by microinjection, patch pipette, or pinocytic loading agent Load in cell media
Format 500 µg 50 µg 500 µg 100 µg
Cat. No. F24180 F24181 F24194 F24195