The Invitrogen™ Attune™ NxT cytometer can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune NxT cytometer.

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Find compatible reagents by excitation source

Each of the tabs below shows the antibody labels and application-based dyes and fluorescent reagents that are compatible with a given laser excitation source.

Violet laser (405 nm) reagents

  Blue Green Orange Red
Emission filter 440/50 nm 512/25 nm 603/48 nm 710/50 nm
Channel name VL1 VL2 VL3 VL4
Compatible fluorescent proteins ▪ Sirius
▪ Azurite
▪ eCFP
▪ eBFP
▪ mTarquoise
▪ Cerulean
▪ vGFP    
Antibody labels ▪ Alexa Fluor 405
▪ Pacific Blue
▪ Pacific Green
▪ Qdot 525
▪ Pacific Orange
▪ Qdot 605
▪ Qdot 705
Viability dyes (compatible with fixation) ▪ LIVE/DEAD Fixable Violet ▪ LIVE/DEAD Fixable Aqua ▪ LIVE/DEAD Fixable Yellow  
Viability dyes (non-fixed cells) ▪ DAPI
▪ SYTOX Blue
  ▪ PI  
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Violet      
DNA content/
cell cycle dyes (fixed cells)
▪ FxCycle Violet      
Apoptosis dyes ▪ Annexin V AF350
▪ PO-PRO-1
▪ Violet Ratiometric Probe (F2N12S) ▪ Violet Ratiometric Probe (F2N12S)  
Cell proliferation ▪ Click-iT Plus Edu Pacific Blue
▪ CellTrace Violet
     
ROS detection        
Phagocytosis        

Blue laser (488 nm) reagents

Color Green Orange Orange Far Red Near IR
Emission filter 530/30 nm 574/26 nm 590/40 nm 695/40 nm 780/60 nm
Channel BL1 BL2
(without yellow laser present)
BL2
(with yellow laser present)
BL3 BL4
Compatible fluorescent proteins

▪ eGFP
▪ Emerald
▪ eYFP

▪ eYFP
▪ mCitrine
▪ Venus
     
Antibody labels ▪ Alexa Fluor 488
▪ FITC
▪ Alexa Fluor 546
▪ Qdot 565
▪ Qdot 585
▪ PE
▪ PE-Alexa Fluor 610
▪ PE-Texas Red
▪ Qdot 585
▪ PE
▪ PE-Alexa Fluor 700
▪ PE-Cy®5.5
▪ PerCP
▪ PerCP-Cy®5.5
▪ Qdot 705
▪ Tri-Color
▪ Pe-Cy®7
▪ PE-Alexa Fluor 750
▪ Qdot 800
Viability dyes (compatible with fixation) ▪ LIVE/DEAD Fixable Green   ▪ LIVE/DEAD Fixable Red    
Viability dyes (non-fixed cells) ▪ SYTOX Green ▪ PI
▪ SYTOX Orange
▪ 7-AAD
▪ PI
▪ SYTOX Orange
▪ SYTOX AADVanced
▪ 7-AAD
▪ PI
▪ SYTOX AADVanced
 
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Green
▪ Vybrant DyeCycle Orange     ▪ Vybrant DyeCycle Ruby
DNA content/
cell cycle dyes (fixed cells)
    ▪ FxCycle PI/Rnase    
Apoptosis dyes ▪ Annexin V AF488
▪ MitoProbe JC-1
▪ CellEvent Caspase-3/7 Green
▪ anti-PARP-FITC
▪ YO-PRO-1 iodide
▪ APO-BrdU TUNEL - Alexa Fluor 488
▪ MitoProbe Transition Pore
▪ MitoProbe DiOC2(3)
▪ Annexin V FITC
▪ Annexin V PE
▪ TMRE
▪ TMRM
▪ MitoProbe JC-1
▪ Annexin V PE
▪ TMRE
▪ TMRM
   
Cell proliferation ▪ Click-iT Plus Edu Alexa Fluor 488
▪ CellTrace CFSE
       
ROS detection CellROX Green        
Phagocytosis ▪ pHrodo Green E. coli BioParticles
▪ pHrodo Green S. aureus BioParticles
▪ pHrodo Red E. coli BioParticles
▪ pHrodo Red Phagocytosis
     

Green laser (532 nm) reagents

Color Orange Red Far Red Near IR
Emission filter 585/16 nm 620/15 nm 695/40 nm 780/60 nm
Channel GL2 GL2 GL3 GL4
Compatible fluorescent proteins ▪ tdTomato
▪ YFP
▪ tmCherry    
Antibody labels

▪ Alexa Fluor 532 ▪ Alexa Fluor 555
▪ DsRed
▪ Qdot 565
▪ Qdot 605
▪ R-phycoerythrin (R-PE, PE)

▪ Alexa Fluor 568
▪ Alexa Fluor 594
▪ Qdot 605
▪ PE-Texas Red™

▪ Qdot 705
▪ PE-Alexa Fluor 700
▪ TRI-COLOR (PE-Cy5)
▪ PE-Cy5.5

▪ Qdot 800
▪ PE-Cy7

Viability dyes (compatible with fixation)   ▪ LIVE/DEAD Fixable Red    
Viability dyes (non-fixed cells) ▪ SYTOX Orange ▪ SYTOX AADVanced
▪ PI
▪ SYTOX AADVanced  
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Orange ▪ Vybrant DyeCycle Orange ▪ Vybrant DyeCycle Ruby  
DNA content/
cell cycle dyes (fixed cells)
  ▪ FxCycle PI/Rnase    
Apoptosis dyes ▪ Annexin V, R-PE
▪ Annexin V, Alexa Fluor™ 555
▪ MitoTracker™ Orange CMTMRos
▪ Annexin V, Alexa Fluor™ 568
▪ Annexin V, Alexa Fluor™ 594
▪ MitoTracker™ Red MNXRos
   
Cell proliferation ▪ CellTrace™ Yellow

▪ Click-iT Plus EDU
▪ Alexa Fluor™ 594

   
ROS detection ▪ CellROX™ Orange      
Phagocytosis ▪ pHrodo Red E. coli BioParticles
     

Yellow laser (561 nm) reagents

Color Orange Red Far red Near IR
Emission filter 585/16 nm 620/15 nm 695/40 nm 780/60 nm
Channel YL1 YL2 YL3 YL4
Compatible fluorescent proteins ▪ mOrange
▪ RFP
▪ dTomato
▪ mCherry
▪ DsRed
▪ mKate
▪ mStrawberry
   
Antibody labels ▪ Alexa Fluor 568
▪ PE
▪ Qdot 565
▪ Qdot 585
▪ Alexa Fluor 594
▪ PE- Alexa Fluor 610
▪ PE-Texas Red
▪ PE-Alexa Fluor 700
▪ PE-Cy®5.5
▪ Qdot 705
▪ TRI-COLOR
▪ PE-Alexa Fluor 750
▪ PE-Cy®7
▪ Qdot 800
Viability dyes (compatible with fixation)        
Viability dyes (non-fixed cells) ▪ PI
▪ SYTOX Orange
▪ 7-AAD
▪ PI
▪ SYTOX AADvanced
▪ SYTOX Orange
▪ 7-AAD
▪ PI
▪ SYTOX AADvanced
 
DNA content/cell cycle dyes (live cells) ▪ Vybrant DyeCycle Orange     ▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells)   ▪ FxCycle PI/Rnase    
Apoptosis dyes ▪ Annexin V AF568
▪ Annexin V AF594
▪ MitoTracker Red CMXRos
▪ MitoTracker Orange CMTMRos
▪ TMRM
     
Cell proliferation        
ROS detection ▪ CellROX Orange      
Phagocytosis        

Red (637 nm) laser reagents

Color Far Red Far Red Near IR
Emission filter 670/14 nm 720/30 nm 780/60 nm
Channel RL1 RL2 RL3
Compatible fluorescent proteins      
Antibody labels ▪ APC
▪ Alexa Fluor 647
▪ Alexa Fluor 660
▪ Qdot 655
▪ Alexa Fluor 680
▪ Alexa Fluor 700
▪ APC-Alexa Fluor 700
▪ Qdot 705
▪ APC-Alexa Fluor 750
▪ APC-Cy® 7
▪ Qdot 800
Viability dyes (compatible with fixation) ▪ LIVE/DEAD Fixable Far Red   ▪ LIVE/DEAD Fixable Near-IR
Viability dyes (non-fixed cells) ▪ SYTOX Red    
DNA content/cell cycle dyes (live cells)     ▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells) ▪ FxCycle Far Red    
Apoptosis dyes ▪ Annexin V APC
▪ Annexin V AF647
▪ Annexin V AF 680
▪ TO-PRO-3
▪ MitoProbe DilC1(5)
   
Cell proliferation ▪ Click-iT Plus Edu Alexa Fluor 647
▪ CellTrace Far Red
   
ROS detection ▪ CellROX Deep Red    
Phagocytosis      

Find compatible reagents according to Attune NxT instrument configuration

The antibody labels and fluorescent proteins that are compatible with each of the seven Attune NxT flow cytometer laser configurations are shown in the expandable sections below.

Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Blue–488 nm 530/30 BL1 Alexa Fluor 488
FITC
 
eGFP
Emerald
eYFP
 
574/26 BL2
Alexa Fluor 546
PE
Qdot 565
Qdot 585
eYFP
mCitrine
Venus
 
695/40 BL3 PE-Alexa Fluor 700
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot 705
TRI-COLOR
 
780/60 BL4 Pe-Cy®7
PE-Alexa Fluor 750
Qdot 800
 
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Blue–488 nm 525/50 BL1 Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
590/40 BL2 PE
PE-Alexa Fluor 610
PE-Texas Red
EYFP
mCitrine
Venus
695/40 BL3 PE-Alexa Fluor 700
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot 705
TRI-COLOR
 
Green–532 nm 575/36 GL1 Alexa Fluor 532
Alexa Fluor 555
DsRed
Qdot 565
Qdot 605
R-phycoerythrin (R-PE, PE)
tdTomato
YFP
620/15 GL2 Alexa Fluor 568
Alexa Fluor 594
Qdot 605
PE-Texas Red
tmCherry
695/40 GL3 Qdot 705
PE-Alexa Fluor 700
TRI-COLOR (PE-Cy®5)
PE-Cy5.5
 
780/60 GL4 Qdot 800
PE-Cy7
 
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Blue–488 nm 530/30 BL1 Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
590/40 BL2 PE
PE-Alexa Fluor 610
PE-Texas Red
Qdot 585
 
695/40 BL3 PE-Alexa Fluor 700
Tri-Color
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot 705 
 
Yellow–561 nm 585/16 YL1 PE  mOrange
RFP
dTomato
620/15 YL2 PE- Alexa Fluor 610
PE-Texas Red
mCherry
DsRed
mKate
mStrawberry
695/40 YL3 PE-Alexa Fluor 700
PE-Cy®5.5
Qdot 705
Tri-Color
 
780/60 YL4 PE-Cy®7
Qdot 800
 
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Violet–405 nm 440/50 VL1 Alexa Fluor 405
Pacific Blue
Sirius
Azurite
eCFP
eBFP
mTarquoise
Cerulean
512/25 VL2  Pacific Green vGFP
603/48 VL3 Pacific Orange
Qdot 605
 
710/50 VL4 Qdot 705  
Blue–488 nm 530/30 BL1 Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
574/26 BL2 Alexa Fluor 546
PE
Qdot 565
Qdot 585
eYFP
mCitrine
Venus
695/40 BL3 PE-Alexa Fluor 700
Tri-Color
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot 705 
 
780/60 BL4 Pe-Cy®7
PE-Alexa Fluor 750
Qdot 800
 
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Blue–488 nm 530/30 BL1 Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
574/26 BL2 Alexa Fluor 546
PE
Qdot 565
Qdot 585
eYFP
mCitrine
Venus
695/40 BL3 PE-Alexa Fluor 700
Tri-Color
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot 705 
 
780/60 BL4 Pe-Cy®7
PE-Alexa Fluor 750
Qdot 800
 
Red–637 nm 670/14 RL1 APC
Alexa Fluor 647
Qdot 655
 
720/30 RL2 Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60 RL3 APC-Alexa Fluor 750
APC-Cy® 7
Qdot 800
 
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Blue–488 nm 525/50 BL1 Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
590/40 BL2 PE
PE-Alexa Fluor 610
PE-Texas Red
eYFP
mCitrine
Venus
695/40 BL3 PE-Alexa Fluor 700
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot 705
TRI-COLOR
 
Green—532 nm 575/36 GL1 Alexa Fluor 532
Alexa Fluor 555
DsRed
Qdot 565
Qdot 605
R-phycoerythrin (R-PE, PE)
tdTomato
YFP
620/15 GL2 Alexa Fluor 568
Alexa Fluor 594
Qdot 605
PE-Texas Red
tmCherry
695/40 GL3 Qdot 705
PE-Alexa Fluor 700
TRI-COLOR (PE-Cy®5)
PE-Cy5.5
 
780/60 GL4 Qdot 800
PE-Cy7
 
Red–637 nm 670/14 RL1 Alexa Fluor 647
APC
Qdot 655
 
720/30 RL2 Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60 RL3 APC-Alexa Fluor 750
APC-Cy®7
 
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Blue–488 nm 525/50 BL1 Alexa Fluor 488
FITC
EGFP
Emerald
EYFP
590/40 BL2 Alexa Fluor 488
FITC
EYFP
mCitrine
Venus
695/40 BL3 PE-Alexa Fluor 700
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot 705
TRI-COLOR
 
Green—532 nm 575/36 GL1 Alexa Fluor 532
Alexa Fluor 555
DsRed
Qdot 565
Qdot 605
R-phycoerythrin (R-PE, PE)
tdTomato
YFP
620/15 GL2 Alexa Fluor 568
Alexa Fluor 594
Qdot 605
PE-Texas Red
tmCherry
695/40 GL3 Qdot 705
PE-Alexa Fluor 700
TRI-COLOR (PE-Cy®5)
PE-Cy®5.5
 
780/60 GL4 Qdot 800
PE-Cy®7
 
Violet—405 nm 440/50 VL1 Alexa Fluor 405
Pacific Blue
Azurite
Cerulean
EBFP
ECFP
mTurquoise
512/25 VL2 Pacific Green T-sapphire 
603/48 VL3 Pacific Orange
Qdot 605
 
710/50 VL4 Qdot 705  
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Violet–405 nm 440/50 VL1 Alexa Fluor™ 405
Pacific Blue™
Sirius
Azurite
eCFP
eBFP
mTarquoise
Cerulean
512/25 VL2  Pacific Green™ vGFP
603/48 VL3 Pacific Orange™
Qdot™ 605
 
710/50 VL4 Qdot™ 705  
Blue–488 nm 530/30 BL1 Alexa Fluor™ 488
FITC
eGFP
Emerald
eYFP
590/40 BL2 PE
PE-Alexa Fluor™ 610
PE-Texas Red™ 
Qdot™ 585
 
695/40 BL3 PE-Alexa Fluor™ 700
Tri-Color™
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot™ 705 
 
Yellow–561 nm 585/16 YL1 PE  mOrange
RFP
dTomato
620/15 YL2 PE- Alexa Fluor 610
PE-Texas Red™
mCherry
DsRed
mKate
mStrawberry
695/40 YL3 PE-Alexa Fluor™ 700
PE-Cy®5.5
Qdot™ 705
Tri-Color™
 
780/60 YL4 PE-Cy®7
Qdot™ 800
 
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Violet–405 nm 440/50 VL1 Alexa Fluor™ 405
Pacific Blue™
Sirius
Azurite
eCFP
eBFP
mTarquoise
Cerulean
512/25 VL2  Pacific Green™ vGFP
603/48 VL3 Pacific Orange™
Qdot™ 605
 
710/50 VL4 Qdot™ 705  
Blue–488 nm 530/30 BL1 Alexa Fluor™ 488
FITC
eGFP
Emerald
eYFP
574/26 BL2 Alexa Fluor 546
PE
Qdot 565
Qdot 585
eYFP
mCitrine
Venus
695/40 BL3 PE-Alexa Fluor™ 700
Tri-Color™
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot™ 705 
 
780/60 BL4 Pe-Cy®7
PE-Alexa Fluor™ 750
Qdot™ 800
 
Red–637 nm 670/14 RL1 APC
Alexa Fluor® 647
Qdot™ 655
 
720/30 RL2 Alexa Fluor™ 680
Alexa Fluor™ 700
APC-Alexa Fluor™ 700
Qdot™ 705
 
780/60 RL3 APC-Alexa Fluor™ 750
APC-Cy® 7
Qdot™ 800
 
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Violet–405 nm 440/50 VL1 Alexa Fluor 405
Pacific Blue
Azurite
Cerulean
EBFP
ECFP
mTurquoise
512/25 VL2 Pacific Green



T-Sapphire
603/48 VL3 Pacific Orange
Qdot 605
 
710/50 VL4 Qdot 705  
Blue–488 nm 525/50 BL1 Alexa Fluor 488
FITC
EGFP
Emerald
EYFP
590/40 BL2 PE
PE-Alexa Fluor 610
PE-Texas Red
EYFP
mCitrine
Venus
695/40 BL3 PE-Alexa Fluor 700
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot 705
 
Green—532 nm 575/36 GL1 Alexa Fluor 532
Alexa Fluor 555
DsRed
Qdot 565
Qdot 605
R-phycoerythrin (R-PE, PE)
tdTomato
YFP
620/15 GL2 Alexa Fluor 568
Alexa Fluor 594
Qdot 605 PE-Texas Red
tmCherry
695/40 GL3 Qdot 705
PE-Alexa Fluor 700
TRI-COLOR (PE-Cy®5)
PE-Cy®5.5
 
780/60 GL4 Qdot 800
PE-Cy®7
 
Red–637 nm 670/14 RL1 Alexa Fluor 647
APC
Qdot 655
 
720/30 RL2 Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60 RL3 APC-Alexa Fluor 750
APC-Cy®7
 
Excitation laser Emission filter (nm) Channel Antibody labels Compatible fluorescent proteins
Violet–405 nm 440/50 VL1 Alexa Fluor™ 405
Pacific Blue™
Sirius
Azurite
eCFP
eBFP
mTarquoise
Cerulean
512/25 VL2  Pacific Green™ vGFP
603/48 VL3 Pacific Orange™
Qdot™ 605
 
710/50 VL4 Qdot™ 705  
Blue–488 nm 530/30 BL1 Alexa Fluor™ 488
FITC
eGFP
Emerald
eYFP
590/40 BL2 PE
PE-Alexa Fluor™ 610
PE-Texas Red™ 
Qdot™ 585
 
695/40 BL3 PE-Alexa Fluor™ 700
Tri-Color™
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot™ 705 
 
Yellow–561 nm 585/16 YL1 PE  mOrange
RFP
dTomato
620/15 YL2 PE- Alexa Fluor 610
PE-Texas Red™
mCherry
DsRed
mKate
mStrawberry
695/40 YL3 PE-Alexa Fluor™ 700
PE-Cy®5.5
Qdot™ 705
Tri-Color™
 
780/60 YL4 PE-Cy®7
Qdot™ 800
 
Red–637 nm 670/14 RL1 APC
Alexa Fluor® 647
Qdot™ 655
 
720/30 RL2 Alexa Fluor™ 680
Alexa Fluor™ 700
APC-Alexa Fluor™ 700
Qdot™ 705
 
780/60 RL3 APC-Alexa Fluor™ 750
APC-Cy® 7
Qdot™ 800
 

Flow Cytometry Panel Design Tool

Our Panel Design tool can help you choose fluorescent antibody conjugates for your flow cytometry panel in three easy steps: 1) pick the antibody species reactivity you want to include in your search, 2) select up to 14 targets of interest (choices include viability dyes), and 3) choose the lasers or fluorophores you want to view (top).

The available antibodies and dyes for your targets will be displayed on a tab for each laser, or you can scroll down the page to find the laser tables. The number of products available will be displayed in each cell; simply click on the number and select the product you want from the list in the pop-up window. Your panel choices will be displayed at the top of the table (bottom). When you are finished choosing products, either print your list or share it via email.

Read the BioProbes 71 article: Flow Cytometry Panel Design: The Basics