Illustration of DNA strand with glowing green fluorophore attached

In this section you will find educational resources such as application notes, webinars, videos, articles and more to assist in your cell proliferation experiments using flow cytometry.

Cell proliferation features

Guided learning

drawing of a brain with text that reads elearning courses

T cell stimulation and proliferation eLearning course

This modular, animated, and narrated eLearning course was developed to provide a succinct, contextual summary of T cell activation and the methods used to measure T cell function. There are knowledge checks throughout the course as well as a practical application session to test what you have learned.

BioProbes article

illustration depicting first through fourth generations of cells dividing and histogram of flow cytometry data showing fluorescence brightness decreasing by half after each division

An evaluation of dyes for tracking cell proliferation by flow and image cytometry

This article reviews the different methods for tracking cell division using fluorescent dyes and flow cytometry. Additionally it presents the different experimental criteria for choosing a particular method or dye for cell proliferation analysis.


Cell proliferation learning resources

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Type Title Categories
BioProbes article (2018)A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studiesantibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability
BioProbes article (2017)An evaluation of dyes for tracking cell proliferation by flow and image cytometrycell proliferation, cell tracking, flow cytometry, fluorescence microscopy/fluorescence imaging
BioProbes article (2016)A guide to studying cell proliferation by generational tracing—Tracking cell division with flow cytometryCellTrace, cell cycle, cell proliferation, cell tracking, flow cytometry
BioProbes article (2014)Click-iT EdU Flow Cytometry Kits— Simplifying cell proliferation analysiscell proliferation, Click-iT, flow cytometry, fluorescence
BioProbes article (2014)Tracking cells through time and space—Choose from fluorescent dyes, Qdot nanocrystals, and targeted fluorescent proteinsCellTrace, cell tracking, fluorescence microscopy/fluorescence imaging, fluorescent proteins,  live cells
BioProbes article (2013)Checking vital signs: Don't let dead cells mislead you—Molecular Probes cell health assays for flow cytometryapoptosis, caspase substrate, cell cycle, cell proliferation, cell tracking, Click-iT, flow cytometry, fluorescence, immunophenotyping, oxidative stress, viability,
BioProbes article (2011)Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometrycell proliferation, Click-iT, cell proliferation, multicolor flow cytometry
BioProbes article (2011)Superior live-cell tracing, generation after generation—The CellTrace Far Red Cell Proliferation Kitcell proliferation, CellTrace, flow cytometry, fluorescence
BioProbes article (2009) A growing list of tools for the violet laser Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability
BioProbes article (2009) Beyond classification—Click-iT EDU outperforms BrdU across all species Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid detection, nucleic acid quantitation, nucleosides, sample preparation
BioProbes article (2008) The picture of cell health Alexa Fluor, cell health, cell proliferation, Click-iT, fluorescent dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, nucleic acid quantitation, nucleosides, reagents
BioProbes article (2007) Breakthrough cell proliferation assay for flow cytometry Alexa Fluor, cell proliferation, Click-iT, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid quantitation
BioProbes article (2007) Improved results are just a click away Alexa Fluor, cell proliferation, Click-iT, flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, multicolor flow cytometry, nucleic acid quantitation, nucleosides
Guided learningT cell stimulation and proliferation eLearning courseantibodies, cell proliferation, cell stimulation, flow cytometry, immunoassays, immunology, immunophenotyping, T cells
ProtocolsBrdU staining protocol for flow cytometry (Invitrogen eBioscience reagents)cell proliferation, flow cytometry
ProtocolsCellTrace CFSE Cell Proliferation Kit protocolcell proliferation, flow cytometry
ProtocolsCellTrace Far Red Cell Proliferation Kit protocolcell proliferation, flow cytometry
ProtocolsCellTrace Violet Cell Proliferation Kit protocolcell proliferation, flow cytometry
ProtocolsStaining cells with eFluor proliferation dyes for flow cytometry (Invitrogen eBioscience reagents)cell proliferation, flow cytometry
Scientific Poster (2016) A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific poster (2014)Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detectioncell proliferation, Click-iT, flow cytometry, immunophenotyping
Scientific Poster (2014) Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing Attune NxT, cell proliferation, Click-iT, multicolor flow cytometry
Scientific Poster (2013) Cells coming to life: Tools for visualizing immune response endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, pH detection, T cell stimulation
Scientific poster (2013) Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2011) The next step in the analysis of lymphocyte proliferationAlexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents
Scientific poster (2010) Analysis of proliferation dynamics in primary and immortalized human cells Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation
Scientific poster (2010) Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2010) Functional characterization of a novel fluorescent dye for proliferation analysis Alexa Fluor, cell proliferation, cells, CellTrace, flow cytometry, fluorescent dyes, violet laser-excited reagents
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imagingAlexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2009) Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation Alexa Fluor, cancer, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes
Scientific poster (2009) Dual pulse labeling using a new thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2009) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
Scientific poster (2008) Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2008) Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-it, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2008) Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry Alexa Fluor, antibodies, ArrayScan, cell cycle, cells, Click-iT, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008) Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability
Scientific poster (2007) Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2006) Novel violet-excited reagents for detection of viability and vitality  Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents
CYToMORE lecture video (2016) Novel reagent solutions for flow cytometry 
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune NxT, flow cytometry assays
Technology videoClick-iT Plus EdU Proliferation Assay 
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging
WebinarAn introduction to flow cytometric analysis, Part 1: Cell proliferation analysis 
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability
WebinarDNA content cell cycle analysis using flow cytometry 
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss: an overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content, selection of DNA dyes for live cell and fixed cell analysis and tips and tricks for consistent results.
cell counting, cell cycle, cell proliferation, flow cytometry