3D illustration of fluorescent dyes attached to surface and intracellular compartment of dead cells

Dead cells often give false positive results, because many reagents will bind to them non-specifically. Therefore, removing dead cells from your flow cytometry data is a critical step to ensure accurate results and analysis. In this section you will find educational resources for analyzing the viability of cells using flow cytometry including application notes, articles, scientific posters, videos, webinars and more.

Cell viability features

On demand webinar

Cell health continuum graphic

An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis

In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.

 

BioProbes article

mastering technical aspects of flow cytometry infographic

A comprehensive resource for state-of-the-art flow cytometry methods

This article provides a review of recently published and extensive guidelines for the use of flow cytometry and cell sorting in immunological studies, as well as an infographic giving tips for key data controls.


Cell viability learning resources

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Type Title Categories
BioProbes article (2018)A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studiesantibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability
BioProbes article (2017)Powering up drug discovery—Cancer biology applications using the Attune NxT Flow Cytometerapoptosis, Attune NxT, cancer, flow cytometer, fluorescence, multicolor flow cytometry, viability
BioProbes article (2016)High-speed cell counting with the Attune NxT Flow Cytometer—Accurately measure cell concentrations by flow cytometryAttune NxT, bacteria, cell concentration, cell counting, flow cytometer, immunophenotyping, microbiology, viability
BioProbes article (2013)Checking vital signs: Don't let dead cells mislead you—Molecular Probes cell health assays for flow cytometryapoptosis, caspase substrate, cell cycle, cell proliferation, cell tracking, Click-iT, flow cytometry, fluorescence, immunophenotyping, oxidative stress, viability,
BioProbes article (2010)Dead cell identification in flow cytometryflow cytometry, fluorescence, immunophenotyping, viability
BioProbes article (2009) A growing list of tools for the violet laser Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability
BioProbes article (2009) Dead cell stains in flow cytometry: A comprehensive analysis apoptosis, fluorescent dyes, viability
BioProbes article (2007) Dead-cell discrimination in a different light flow cytometry, fluorescent dyes, viability
BioProbes article (2006) Analyzing cell culture health and concentration apoptosis, cell counting, fluorescent dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader, particles, viability
Molecular Probes HandbookViability and cytotoxicity assay reagents—Section 15.2cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes HandbookViability and cytotoxicity assay kits for diverse cell types—Section 15.3cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
ProtocolsLIVE/DEAD Fixable Dead Cell Stains protocolflow cytometry, viability
ProtocolsLIVE/DEAD Sperm Viability Kit flow cytometry protocolflow cytometry, viability
ProtocolsLIVE/DEAD Violet Viability/Vitality Kit protocolflow cytometry, viability
ProtocolsSYTOX Dead Cell Stains protocolcell viability, flow cytometry
ProtocolsViability staining protocol for flow cytometry (Invitrogen eBioscience reagents)cell viability, flow cytometry
Scientific poster (2018) Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research
Scientific poster (2018) Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques Attune/Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination
Scientific Poster (2016) Cell mediated cytotoxicity in “untouched” whole blood Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood
Scientific poster (2013) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health apoptosis, ArrayScan, caspase substrates, cell health, cells, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific poster (2009) A simple, fast and quantitative single-step dead-cell indicator for flow cytometry apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability
Scientific poster (2009) Dead cell stains in flow cytometry: A comprehensive analysis apoptosis, cells, flow cytometry, fluorescent dyes, immunophenotyping, viability
Scientific poster (2006) Novel violet-excited reagents for detection of viability and vitality  Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents
CYToMORE lecture video (2016) Novel reagent solutions for flow cytometry 
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune NxT, flow cytometry assays
WebinarAn introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis 
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability