Gateway® Destination Vectors

Versatile Gateway recombination cloning vectors

The Invitrogen Gateway Cloning Technology provides a rapid and highly efficient route to protein expression, functional analysis, and cloning/subcloning of DNA segments. Gateway-compatible destination vectors contain the Gateway recombination sites, allowing rapid, efficient transfer of sequences into a variety of expression systems using site-specific recombination.

Destination vectors selection tool

Destination vector selection guides

Gateway cloning technology is especially noted for its utility in protein expression. The flexibility and diverse selection of Destination vectors and host systems is particularly attractive for multidisciplinary protein expression studies.

Destination vectors for protein expression

Host system for protein expressionGateway Destination vector family
E. colipDEST 14, 15, 17, and 24
pET160 and pET161 DEST vectors
Insect cellsBaculoDirect C-Term Expression
Mammalian cells (constitutive expression)pcDNA mammalian expression vector family
Mammalian cells (regulated expression)pT-REx-DEST30, and pT-REx-DEST31
Mammalian cells (viral delivery)ViraPower Lentiviral Expression Systems

Destination vectors for additional application areas

ApplicationGateway Destination vector family
Antibody or antigen productionChampion pET Expression systems
LocalizationVivid Colors pcDNA GFP Destination vector family
Protein arrayExpressway Plus Expression System
Protein–protein interaction studiesProQuest Two-Hybrid System using Gateway technology
Reporter assayGeneBLAzer pcDNA vector family
RNAiGeneBLAzer pcDNA vector family

Featured Gateway protein expression vectors

High-level inducible mammalian expression
pT-REx-DEST30 andpT-REx-DEST31 are part of the T-REx system, the first inducible mammalian expression system to use the full-length CMV promoter providing high level, regulated expression. There is no toxicity with Thermo Scientific™ T-REx™ because viral transactivators are not used. Easy selection of stable cells using Gibco™ GENETICIN™ is possible due to the presence of the Neomycin resistance gene. Additionally, pT-REx-DEST31 contains a polyhistidine (6xHis) tag allowing for rapid purification on Invitrogen™ ProBond™ resin or detection with an Anti-His Antibody.

Regulated high-level E. coli expression
utilizes the T7/lac promoter for regulated, high-level expression in E. coli. The vector has the pBR322 low-copy origin of replication for minimized basal expression level. A C-terminal V5 epitope and polyhistidine (6xHis) tag allow for detection using an Anti-V5 Antibody and an Anti-His Antibody respectively.

To find additional Destination vectors, see our Vector Selection tool