biomarker analysis 96-well gene expression analysis

Cytochrome P450s (CYPs) and other enzymes metabolize the majority of therapeutic drugs. Changes in the disposition of these enzymes can alter the ADME (absorption, distribution, metabolism, and excretion) properties of drugs. In addition, co-administered drugs can lead to ineffective therapies if the drug-drug interactions (DDI) increase the rate of metabolic clearance of either drug. In order to assess the potential for loss of drug efficacy, ADME/DMPK (Drug Metabolism and Pharmacokinetics) researchers measure induction of genes for drug metabolizing enzymes such as CYPs to support drug discovery programs. A common methodology used is to determine CYP induction via mRNA expression and CYP enzyme activity using cryopreserved human hepatocytes.

QuantiGene Assays enable ADME/DMPK/Toxicology researchers to:

  • Use hepatocytes more efficiently to measure gene expression for multiple drug metabolizing or transporting enzymes in the same well
  • Obtain data on targets when a substrate assay is not available
  • Follow the 2017 FDA draft guidance on in vitro Metabolism- and Transporter-Mediated Drug-Drug Interaction Studies by using mRNA level as an endpoint[1]

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Multiplex ADME/Tox gene expression panel

The QuantiGene Plex ADME Panel targets 50 genes for various phase I and II metabolizing enzymes and phase III transporters, and includes 8 Reference genes. This panel is compatible with all Luminex instruments.

AccessionGene NameSymbol
NM_019093UDP glycosyltransferase 1 family, polypeptide A3UGT1A3
NM_000341solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1SLC3A1
NM_006446solute carrier organic anion transporter family, member 1B1SLCO1B1
NM_000106cytochrome P450, family 2, subfamily D, polypeptide 6CYP2D6
NM_001461flavin containing monooxygenase 5FMO5
NM_003167sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA) -preferring, member 1SULT2A1
NM_002046glyceraldehyde-3-phosphate dehydrogenaseGAPDH
NM_001101actin, betaACTB
NM_000379xanthine dehydrogenaseXDH
NM_017460cytochrome P450, family 3, subfamily A, polypeptide 4CYP3A4
NM_000463UDP glycosyltransferase 1 family, polypeptide A1UGT1A1
NM_000181Glucuronidase, betaGUSB
NM_001159aldehyde oxidase 1AOX1
NM_000773cytochrome P450, family 2, subfamily E, polypeptide 1CYP2E1
NM_003742ATP-binding cassette, sub-family B (MDR/TAP), member 11ABCB11
NM_000291phosphoglycerate kinase 1PGK1
NM_000767cytochrome P450, family 2, subfamily B, polypeptide 6CYP2B6
NM_003057solute carrier family 22 (organic cation transporter), member 1SLC22A1
NM_003194TATA box binding proteinTBP
NM_002021flavin containing monooxygenase 1FMO1
NM_007120UDP glycosyltransferase 1 family, polypeptide A4UGT1A4
NM_004827ATP-binding cassette, sub-family G (WHITE), member 2ABCG2
NM_000770cytochrome P450, family 2, subfamily C, polypeptide 8CYP2C8
NM_000762cytochrome P450, family 2, subfamily A, polypeptide 6CYP2A6
NM_000937polymerase (RNA) II (DNA directed) polypeptide A, 220kDaPOLR2A
NM_000761cytochrome P450, family 1, subfamily A, polypeptide 2CYP1A2
NM_001074UDP glycosyltransferase 2 family, polypeptide B7UGT2B7
NM_000769cytochrome P450, family 2, subfamily C, polypeptide 19CYP2C19
NM_001076UDP glycosyltransferase 2 family, polypeptide B15UGT2B15
NM_021027UDP glycosyltransferase 1 family, polypeptide A9UGT1A9
NM_005502ATP-binding cassette, sub-family A (ABC1), member 1ABCA1
NM_000775cytochrome P450, family 2, subfamily J, polypeptide 2CYP2J2
NM_004605sulfotransferase family, cytosolic, 2B, member 1SULT2B1
NM_007256solute carrier organic anion transporter family, member 2B1SLCO2B1
NM_000194Hypoxanthine phosphoribosyltransferase 1HPRT1
NM_004996ATP-binding cassette, sub-family C (CFTR/MRP), member 1ABCC1
NM_000898monoamine oxidase BMAOB
NM_000777cytochrome P450, family 3, subfamily A, polypeptide 5CYP3A5
NM_001055sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1SULT1A1
NM_000771cytochrome P450, family 2, subfamily C, polypeptide 9CYP2C9
NM_006894flavin containing monooxygenase 3FMO3
NM_001072UDP glycosyltransferase 1 family, polypeptide A6UGT1A6
NM_000499cytochrome P450, family 1, subfamily A, polypeptide 1CYP1A1
NM_019844solute carrier organic anion transporter family, member 1B3SLCO1B3
NM_000240monoamine oxidase AMAOA
NM_000942peptidylprolyl isomerase B (cyclophilin B)PPIB
NM_000392ATP-binding cassette, sub-family C (CFTR/MRP), member 2ABCC2
NM_006516solute carrier family 2 (facilitated glucose transporter), member 1SLC2A1
NM_003049solute carrier family 10 (sodium/bile acid cotransporter family), member 1SLC10A1
NM_000927ATP-binding cassette, sub-family B (MDR/TAP), member 1ABCB1

For Research Use Only. Not for use in diagnostic procedures.