Cytochrome P450s (CYPs) and other enzymes metabolize the majority of therapeutic drugs. Changes in the disposition of these enzymes can alter the ADME (absorption, distribution, metabolism, and excretion) properties of drugs. In addition, co-administered drugs can lead to ineffective therapies if the drug-drug interactions (DDI) increase the rate of metabolic clearance of either drug. In order to assess the potential for loss of drug efficacy, ADME/DMPK (Drug Metabolism and Pharmacokinetics) researchers measure induction of genes for drug metabolizing enzymes such as CYPs to support drug discovery programs.

QuantiGene Assays enable ADME/DMPK/Toxicology researchers to:

  • Use hepatocytes more efficiently to measure gene expression for multiple drug metabolizing or transporting enzymes in the same well
  • Obtain data on targets when a substrate assay is not available
  • Follow the 2017 FDA draft guidance on in vitro Metabolism- and Transporter-Mediated Drug-Drug Interaction Studies by using mRNA level as an endpoint[1]

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Omeprazole concentration response curves in lot CDP
 Click image to enlarge
Figure1. Omeprazole concentration response curves and EC50 vlaues for various ADME genes.

Multiplex ADME/Tox gene expression panel

The QuantiGene Plex ADME Panel targets 42 genes for various phase I and II metabolizing enzymes and phase III transporters, and includes 8 Reference genes. This panel is compatible with Luminex MAGPIX bead regions.

If you would like to redesign this panel:

  1. Download the template containing all genes listed below.
    • Click save after the file opens in new window.
  2. Upload the template into our QuantiGene Plex Panel configurator
  3. Add or remove genes according to your needs, then submit the request


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Accession Gene Name Symbol
NM_019093 UDP glycosyltransferase 1 family, polypeptide A3 UGT1A3
NM_000341 solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1 SLC3A1
NM_006446 solute carrier organic anion transporter family, member 1B1 SLCO1B1
NM_000106 cytochrome P450, family 2, subfamily D, polypeptide 6 CYP2D6
NM_001461 flavin containing monooxygenase 5 FMO5
NM_003167 sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA) -preferring, member 1 SULT2A1
NM_002046 glyceraldehyde-3-phosphate dehydrogenase GAPDH
NM_001101 actin, beta ACTB
NM_000379 xanthine dehydrogenase XDH
NM_017460 cytochrome P450, family 3, subfamily A, polypeptide 4 CYP3A4
NM_000463 UDP glycosyltransferase 1 family, polypeptide A1 UGT1A1
NM_000181 Glucuronidase, beta GUSB
NM_001159 aldehyde oxidase 1 AOX1
NM_000773 cytochrome P450, family 2, subfamily E, polypeptide 1 CYP2E1
NM_003742 ATP-binding cassette, sub-family B (MDR/TAP), member 11 ABCB11
NM_000291 phosphoglycerate kinase 1 PGK1
NM_000767 cytochrome P450, family 2, subfamily B, polypeptide 6 CYP2B6
NM_003057 solute carrier family 22 (organic cation transporter), member 1 SLC22A1
NM_003194 TATA box binding protein TBP
NM_002021 flavin containing monooxygenase 1 FMO1
NM_007120 UDP glycosyltransferase 1 family, polypeptide A4 UGT1A4
NM_004827 ATP-binding cassette, sub-family G (WHITE), member 2 ABCG2
NM_000770 cytochrome P450, family 2, subfamily C, polypeptide 8 CYP2C8
NM_000762 cytochrome P450, family 2, subfamily A, polypeptide 6 CYP2A6
NM_000937 polymerase (RNA) II (DNA directed) polypeptide A, 220kDa POLR2A
NM_000761 cytochrome P450, family 1, subfamily A, polypeptide 2 CYP1A2
NM_001074 UDP glycosyltransferase 2 family, polypeptide B7 UGT2B7
NM_000769 cytochrome P450, family 2, subfamily C, polypeptide 19 CYP2C19
NM_001076 UDP glycosyltransferase 2 family, polypeptide B15 UGT2B15
NM_021027 UDP glycosyltransferase 1 family, polypeptide A9 UGT1A9
NM_005502 ATP-binding cassette, sub-family A (ABC1), member 1 ABCA1
NM_000775 cytochrome P450, family 2, subfamily J, polypeptide 2 CYP2J2
NM_004605 sulfotransferase family, cytosolic, 2B, member 1 SULT2B1
NM_007256 solute carrier organic anion transporter family, member 2B1 SLCO2B1
NM_000194 Hypoxanthine phosphoribosyltransferase 1 HPRT1
NM_004996 ATP-binding cassette, sub-family C (CFTR/MRP), member 1 ABCC1
NM_000898 monoamine oxidase B MAOB
NM_000777 cytochrome P450, family 3, subfamily A, polypeptide 5 CYP3A5
NM_001055 sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1 SULT1A1
NM_000771 cytochrome P450, family 2, subfamily C, polypeptide 9 CYP2C9
NM_006894 flavin containing monooxygenase 3 FMO3
NM_001072 UDP glycosyltransferase 1 family, polypeptide A6 UGT1A6
NM_000499 cytochrome P450, family 1, subfamily A, polypeptide 1 CYP1A1
NM_019844 solute carrier organic anion transporter family, member 1B3 SLCO1B3
NM_000240 monoamine oxidase A MAOA
NM_000942 peptidylprolyl isomerase B (cyclophilin B) PPIB
NM_000392 ATP-binding cassette, sub-family C (CFTR/MRP), member 2 ABCC2
NM_006516 solute carrier family 2 (facilitated glucose transporter), member 1 SLC2A1
NM_003049 solute carrier family 10 (sodium/bile acid cotransporter family), member 1 SLC10A1
NM_000927 ATP-binding cassette, sub-family B (MDR/TAP), member 1 ABCB1

Resources

Application notes

Publications

References