QuantiGene™ RNA assays are hybridization-based assays that utilize a branched DNA technology for signal amplification for the direct quantitation of gene expression transcripts.  They provide a more robust and accurate measurement compared to qPCR. The QuantiGene Plex and QuantiGene Singleplex RNA assays are designed to quantitatively measure RNA directly from cell or tissue homogenates without RNA purification or enzymatic manipulation, thereby avoiding inefficiencies and variability introduced by or errors inherent to these processes.

The QuantiGene™ Plex Assay utilizes xMAP Luminex beads for multiplexing of 3 to 80 RNA targets followed by branched DNA-based signal amplification. The assay is performed in a 96-well plate and signal is detected using a Luminex instrument.

The QuantiGene™ Singleplex assays utilize a sandwich nucleic acid hybridization method followed by branched DNA-based signal amplification. The Singleplex Assay is performed in a 96-well plate and signal is detected using a standard luminometer.  The Singleplex HT Assay is performed in a higher-throughput 384-well plate.


Resources

Video: QuantiGene Plex – How It Works
Video: QuantiGene Singleplex – How It Works