Perform photometric research applications such as DNA, RNA and protein analysis with the Thermo Scientific™ Multiskan™ GO Microplate Spectrophotometer. The Multiskan GO features a broad wavelength range (including UV area), path length correction and a fast reading speed. Our intuitive user interface lets you run quick measurements directly from the instrument. Or, use our comprehensive, yet easy-to use Thermo Scientific™ SkanIt™ Software for more demanding assays.

This protocol library includes ready-made software sessions that can be used to run commercially available or commonly well-known assays with the Thermo Scientific™ Multiskan™ FC microplate photometer. The files contain sessions for the Thermo Scientific™ SkanIt™ Software version 3.1 (or older), which is the PC software used to control the instrument and to perform calculations to measurement data. There are also protocols available for the internal software of the Multiskan FC, as the instrument can also be used without a PC.

These SkanIt Software sessions can be downloaded from this website and imported to the database of the SkanIt Software for Multiskan FC. The downloaded internal software protocols can be imported to the internal software of Multiskan FC. The software sessions and protocols are instrument-specific, therefore, they cannot be imported to the software of any other instrument. The session and protocol files cannot be viewed outside the SkanIt Software or the internal software.

All the sessions and protocols are made for 96-well microplate format.

The sessions and protocols have been made according to the available kit instructions. The user performing the assay has the ultimate responsibility to verify that the definitions, parameters, and calculations of the used session match the kit instructions and the assay requirements. Thermo Fisher Scientific will not assume any responsibility for the results of the assays performed with these software sessions and protocols.

Read first the instructions on downloading the SkanIt Software sessions or the internal software protocols of the Multiskan FC.

Note:  SkanIt software sessions on the protocol library are compatible with the SkanIt software for Multiskan FC version 3.1 or older only. Sessions for the 4th generation SkanIt software are under development and will be available soon. 

Assay  Description  SkanIt software session download  Internal software protocol download 
Promega CellTiter 96 AQueous One Solution Cell Proliferation Assay Determining the number of viable cells in proliferation or cytotoxicity assays  
AniLabsystems HIV EIA Detection of antibodies to HIV
Thermo Scientific Coomassie (Bradford) Protein Assay Total protein quantitation  
Thermo Scientific Pierce 660 nm Protein Assay Total protein quantitation
Thermo Scientific BCA Protein Assay Total protein quantitation
Thermo Scientific Modified Lowry Protein Assay Total protein quantitation  
Thermo Scientific Human TNF Alpha ELISA Quantification of human Tumor Necrosis Factor a
Thermo Scientific Human IL-8 ELISA Quantification of human Interleikin-8  
Thermo Scientific Human GM-CSF ELISA Quantification of human Granulocyte-Macrophage Colony-Stimulating Factor  
Thermo Scientific Human IL-1 alpha ELISA Quantification of human Interleukin-1 a   
Thermo Scientific Human IL-1 beta ELISA Quantification of human Interleukin-1b   
Thermo Scientific Human IL-2 ELISA Quantification of human Interleukin-2  
Thermo Scientific Human IL-4 ELISA Quantification of human Interleukin-4  
Thermo Scientific Human IL-5 ELISA Quantification of human Interleukin-5  
Thermo Scientific Human IL-6 ELISA Quantification of human Interleukin-6  
Thermo Scientific Human IL-10 ELISA Quantification of human Interleukin-10  
Thermo Scientific Human IL-12 p70 ELISA Quantification of human Interleukin-12 (p70)  
Thermo Scientific Human IL-13 ELISA Quantification of human Interleukin-13  
Thermo Scientific Human IL-16 ELISA Quantification of human Interleukin-16  
Thermo Scientific Human MCP-1 ELISA Quantification of human Monocyte Chemoattractant Protein-1  
Thermo Scientific Human RANTES ELISA Quantification of human RANTES  
Thermo Scientific Human sIL-2R ELISA Quantification of human soluble Interleukin-2 Receptor  
Thermo Scientific Human VEGF ELISA Quantification of human VEGF  
Thermo Scientific Mouse GM-CSF ELISA Quantification of mouse Granulocyte-Macrophage Colony-Stimulating Factor  
Thermo Scientific Mouse IL-1 alpha ELISA Quantification of mouse Interleukin-1a   
Thermo Scientific Mouse IL-1 beta ELISA Quantification of mouse Interleukin-1b   
Thermo Scientific Mouse IL-2 ELISA Quantification of mouse Interleukin-2  
Thermo Scientific Mouse IL-4 ELISA Quantification of mouse Interleukin-4  
Thermo Scientific Mouse IL-5 ELISA Quantification of mouse Interleukin-5  
Thermo Scientific Mouse IL-6 ELISA Quantification of mouse Interleukin-6  
Thermo Scientific Mouse IL-10 ELISA Quantification of mouse Interleukin-10  
Thermo Scientific Mouse IL-12 ELISA Quantification of mouse Interleukin-12  
Thermo Scientific Mouse IL-12 p40 ELISA Quantification of mouse Interleukin-12 (p40)  
Thermo Scientific Mouse MCP-1 ELISA Quantification of mouse Monocyte Chemoattractant Protein-1  
Thermo Scientific Mouse Soluble ICAM-1(cd54) ELISA Quantification of mouse Soluble Intercellular Adhesion Molecule-1  
Thermo Scientific Mouse TNF alpha ELISA Quantification of mouse Tumor Necrosis Factor a   
Thermo Scientific Mouse IL-12 Total ELISA Quantification of total mouse Interleukin-12  
Thermo Scientific Porcine IFN gamma ELISA Quantification of porcine Interferon y   
Thermo Scientific Rat IFN gamma ELISA Quantification of rat Interferon y   
Thermo Scientific Rat IL-1 beta ELISA Quantification of rat Interleukin-1b   
Thermo Scientific Rat IL-10 ELISA Quantification of rat Interleukin-10  
Thermo Scientific Rat MCP-1 ELISA Quantification of rat Monocyte Chemoattractant Protein-1
Thermo Scientific Rat TNF alpha ELISA Quantification of rat Tumor Necrosis Factor a   
Thermo Scientific Human IL-12 Total ELISA Quantification of human Interleukin-12 (both p70 and p40 forms)