Browse through overviews of a broad array of applications including protein and nucleic acid quantification assays, ELISA assays, cell signaling assays, cell viability, apoptosis, and more.


Microplate assays

Cell viability microplate assay kit

Microplate assays provide information on entire cell populations rather than tracking the behavior of individual cells. We offer microplate assays for whole cells and assays performed on disrupted cells or cell lysates. Metabolic activity is commonly used as a viability indicator, but for some applications it can be important to assess viability independent of metabolic state, and appropriate assays are listed below. In some cases, cells are required for additional downstream functional analysis, and alamarBlue HS is an excellent nontoxic indicator of viability.

 

Uses/Examples:

Results for alamarBlue assay for cell viability

Cell proliferation microplate assay kit

Cell proliferation analyses are crucial for cell growth and differentiation studies and are often used to evaluate both compound toxicity and inhibition of tumor cell growth during drug development. Proliferation measurements in microplate assays are typically based on average DNA content or cellular metabolism, or they quantify DNA synthesis.

 

Uses/Examples:

CyQuant direct assay results for cell proliferation

Nucleic Acid Quantification microplate assay kit

Accurate, efficient nucleic acid quantitation is critical for cellular and molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. These applications include next-generation sequencing, transfection, real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR, northern blot analysis, S1 nuclease assays, RNase protection assays, and cDNA library preparations.

 

Uses/Examples:

Quant-It assay picture for nucleic acid quantification

Protein Quantitation microplate assay kit

Quantitating protein samples is an important step before gel electrophoresis or Western blot analysis, and for measuring bound versus free protein levels in protein binding assays. The choice of assay format is often based on other components in the sample mixture. We offer many popular choices including modified Lowry, Coomassie (Bradford) and fluorometric protein assays.

 

Uses/Examples:

Pierce Protein 660 assay output using SkanIt software

ELISA assay kit

ELISA assays are an important tool for the quantitative analysis specific proteins from a wide variety of samples. We offer over 2000 highly referenced ELISA assays to detect cytokines, phosphoproteins, oncogenes, and a wide variety of biomarkers. Most assays are available is a variety of formats including pre-coated, bulk and matched pairs to maximize your time and budget.

 

Uses/Examples: 

Results for Prostaglandin ELISA assay

Enzyme activity microplate assay kit

EnzCheck Assays provide a rapid and convenient way to measure a range of enzyme activities using fluorescent reporters in a robust assay format. Each assay kit provides the necessary assay buffers, uses a simple protocol and defines the optimum wavelength for sensitive detection. The microplate format is convenient for high throughput analysis using a 200 µL assay volume. We offer assays for proteases, collagenases, elastases, lysozyme, and reverse transcriptase.

 

Uses/Examples:

Protease assay principle

Cell signaling and lipids microplate assay kit

Measuring phosphates and phosphatase activity in solution is fundamental to an understanding of cellular dynamics in multiple models. We offer a series of sensitive assays with fluorescent readout to address a diverse assortment of analytes.

 

Uses/Examples:

Phosphate assay principle for cell signaling and lipids

Enzyme activity microplate assay kit

A distinctive feature of the early stages of apoptosis is the activation of caspase enzymes, which participate in the cleavage of protein substrates and in the subsequent disassembly of the cell. We offer a series of caspase assays that allow the simple detection of active caspases in living cells in real time or in cellular lysates or extracts.

 

Uses/Examples:

Caspase 3_7 assay results for caspase activity

Metabolites and analytes microplate assay kit

We've developed Amplex Red reagent–based assays for the detection of a diverse assortment of analytes, including glucose, galactose, cholesterol, glutamic acid, xanthine (or hypoxanthine), uric acid, choline and acetylcholine, as well as hydrogen peroxide.

 

Uses/Examples:

NA-Star assay for metabolites and analytes

Reactive Oxygen Species microplate assay kit

Generation of reactive oxygen species (ROS) is inevitable for aerobic organisms and, in healthy cells, occurs at a controlled rate. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as a variety of pathological events, including atherosclerosis, carcinogenesis, ischemia reperfusion injury, and neurodegenerative disorders.

 

Uses/Examples:

CellROX Green assay protocol

Microplate assay using ion indicators kit

We’ve developed a number of Molecular Probes ion indicators to track calcium and other ion concentrations with intense fluorescent signals and a range of wavelength options. Regulating ionic gradients is critical for most cellular functions and measuring ionic concentrations with both spatial and temporal resolution has become critical in research ranging from drug discovery to studies of neuronal function.

 

Uses/Examples:

: Fura-2 ion indicator assay results for ion indicator assay

β-Galactosidase Activity microplate assay kit

The E. coli lacZ gene, encoding β-D-galactosidase, is extensively used as a reporter gene for detecting the expression of recombinant fusion genes and for monitoring transfection efficiency in mammalian, yeast, and bacterial cells. Although mammalian cells do contain β-galactosidases, they are generally lysosomal enzymes with low pH optima and therefore exhibit low activity at neutral pH. Combining this with the fact that E. coli β-D-galactosidase has a high turnover rate, the enzyme can be detected at very low levels, making it a sensitive reporter of gene expression.

 

Uses/Examples:

Kinetic beta-gal activity results for β-Galactosidase Activity

For Research Use Only. Not for use in diagnostic procedures.