The Applied Biosystems TaqMan Array Card (TAC) provides the gold-standard performance of our probe-based TaqMan Assays in an easy and efficient mid- to high-throughput format adoptable by any lab. Experimental throughput in research laboratories continues to increase, and so does the need for simple and efficient ways to run tests. Researchers are often left to choose between cumbersome, time-consuming manual approaches and costly automation. TaqMan Array cards provide an alternative that enables highly reproducible and sensitive results with higher throughput, without the expense of liquid-handling robotics or error potential of excessive pipetting.
The TaqMan Array Card is a 384-well microfluidic card designed to perform up to 384 simultaneous real-time PCR (qPCR) reactions and can support 1–8 samples, 11–380 assay targets, and 1–4 replicates per card. There are four content options available, including inventoried (preconfigured), made-to-order flexible, custom, or specialty designed arrays. Regardless of which configuration you choose, the cards arrive pre-aliquoted with the selected dried-down TaqMan Assays (primers and probe sets)—the only step left is to add your sample and master mix.
Widely cited in publications, TaqMan Array cards are ideal for medium-to-high throughput validation and profiling studies, whether you are researching cancer, stem cells, inflammation, or infectious diseases. Use the cards to validate tens or hundreds of initial hits generated from microarrays or next-generation sequencing, or use TACs for screening biomarkers and toxicology panels or analyzing pathways, target classes, and complete disease sets.
Watch this short video to see how easy it is to load a TaqMan Array Card, featuring Nathan Zuzow, PhD, Field Application Scientist at Thermo Fisher Scientific.
TaqMan Array cards are optimal for medium to large projects involving approximately 50 samples and fewer than 97 targets. They have the simplest and quickest qPCR bench workflow: pipette into 8 ports, then let a centrifuge distribute the reaction mixture evenly through all 384-wells. Cards use the least amount of master mix (~400 µL per card) and are the best option for those with limited sample material.
While used extensively in gene expression applications, TaqMan Array cards are also available for additional applications as shown here:
Preconfigured, off-the-shelf cards containing a set of curated TaqMan Assays relevant for specific pathways, biomarker sets, or disease states.
Explore a list of inventoried cards or use the easy search tool below.
Select a preconfigured pathway panel then modify assay content to suit your needs.
Explore a list of flexible, made-to-order cards or use the easy search tool below.
Configure your card from scratch using our simple online tool and choose from > 2.8 million predesigned TaqMan Gene Expression or microRNA assays.
Custom design your card using any combination of GEX, SNP, CNV, miRNA, and custom assays, configured to your specifications.
|Dispense 100 μL of nucleic acid and master mix into each fill port||Centrifuge the TaqMan Array cards for 2 minutes||Seal off the main channels with the sealer and cut the strip||Perform amplification and analysis on a compatible Applied Biosystems qPCR system|
For Research Use Only. Not for use in diagnostic procedures.