Custom Assays for Gene Expression 

1. Review our Design and Ordering Guide (PDF) for best results.
2. Use the Custom TaqMan® Assay Design Tool to input sequences, submit for design, and order Custom Assays.

Each custom assay is a mix of forward primer, reverse primer, and FAM™ or VIC™ dye-labeled TaqMan® MGB probe. Designed to run under universal oligo concentration and thermal cycling conditions for two-step RT-PCR, these assays are simple to use. Just add TaqMan® Master Mix and your cDNA sample to generate sensitive, reproducible, and truly quantitative RNA expression data.

Custom TaqMan® Assay Design Tool

Visit our Specialty TaqMan Assays & Oligos webpage to learn more

There are two custom assay options to meet RNA research needs: Custom TaqMan Gene Expression Assays and Custom Plus Applied Biosystems TaqMan RNA Assays. Both allow a target sequence to be submitted to our TaqMan Assay design pipeline, which then uses the input sequence to design custom primer and probe sequences. See the key differences between the two options below:

Feature	Custom Plus TaqMan RNA Assay	Custom TaqMan Gene Expression Assay
Bioinformatic analysis performed on input target sequences
In silico QC performed on assay sequences
Specificity option for gene-level or transcript-level detection
Contains TaqMan MGB probe and 2 unlabeled primers
Runs under universal thermal cycling conditions
Assay sequences provided
Available for any species	*Available for any species with genome information in our database
Automatic check for predesigned TaqMan Assays which may detect input sequence

Custom Plus TaqMan RNA Assays are designed using the full bioinformatic power of the TaqMan assay design pipeline, which uses proprietary algorithms that have generated millions of assay designs. If bioinformatics or QC checks are not needed on the sequences, then the standard custom TaqMan gene expression assays are recommended. For Custom Plus, select to have bioinformatic analysis performed on your sequences.

Submitting a target sequence is straightforward with the Custom Assay Design Tool. Researchers can easily select their bioinformatic analysis and assay specificity preferences before entering their target sequence. All sequence submissions are kept completely confidential, with no sharing of target or assay sequences with third parties. For those without a pre-defined input sequence, the Custom Assay Design Tool provides powerful search capabilities, allowing users to find sequences by keyword (such as gene symbol or accession number) or by genomic location across a wide range of well-studied model species, including human, mouse, rat, dog, cow, pig, Arabidopsis, and more.

Learn more:  Custom TaqMan assays design and ordering guide

Order custom assays

Please see below to order Custom TaqMan Gene Expression Assays.

1. Bergmann, O; Zdunek, S.; Alkass, K; et al. “Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover.” Exp Cell Res 2 (2011): 188–194. 
2. Hevir, N.; Sinkovec, J.; Rizner, T.L. “Disturbed expression of phase I and phase II estrogen-metabolizing enzymes in endometrial cancer: lower levels of CYP1B1 and increased expression of S-COMT.” Mol Cell Endocrinol 1 (2011): 158–167. 
3. Colazzo, F.; Sarathchandra, P.; Smolenski, R.T.; et al. “Extracellular matrix production by adipose-derived stem cells: implications for heart valve tissue engineering.” Biomaterials 1 (2011): 119–127.