iBind Flex Western Device

Flexible formats–Less antibody–Reproducible results

The Invitrogen™ iBind™ Flex Western System offers flexible blot processing to optimize antibody use, easily change formats, and reduce hands-on time. Simply load primary and secondary antibody solutions and wash solutions into the device, then walk away, because the system automaticallyperforms all immunodetection steps using sequential lateral flow technology, a simple form of capillary action. In less than 3 hours, the blot is ready for final detection. The iBind Flex Western System delivers flexible solutions with automated convenience.

  • Flexibility—process up to one midi blot, two mini blots or six vertically cut strips using the same or different conditions
  • Compatibility—use nitrocellulose or PVDF membranes, directly labeled primary or secondary antibody detection (AP, HRP or fluorescent labeled)
  • Cost savings—use up to 80% less primary antibody than with traditional tray-based incubation steps for western blotting

Order now  Request a demo  Download the manual

Love it or return it!

Try it for 30 days. We’re confident that you’ll love how the iBind Flex Western Device works; if you don’t, you can return the device for a full refund.*

To return the device, please contact technical services at 800-955-6288 (select option 4) or ts.proteins@lifetech.com with purchase information, including PO# and purchase date.

*Terms and conditions: 30-day money-back guarantee valid only for customers in the US (excluding Puerto Rico) and Canada who purchase a new, qualifying iBind Flex device (Cat. Nos. SLF2000S and SLF2000 only). Offer valid on qualifying orders received by Life Technologies after 12:00 a.m. ET on April 22, 2016, and no later than 5:00 p.m. ET on December 31, 2016, or until promotional supplies are depleted, whichever comes first. 30-day time period will begin upon receipt of the product by the customer. Upon receipt of a returned product, Customer will receive a product credit to their account for the amount paid for the instrument. Any gifts or promotional items received with the purchase of the instrument must be returned in order to receive a full credit. Life Technologies will deduct the actual retail value of any promotional item not returned from the amount credited to customer's account. Customer is responsible for all shipping and handling fees associated with the return of the instrument under this program. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

Watch the iBind Flex demo video

Future of Westerns: How to tackle your western blot challenges

Description: Watch our webinar on “Automated Protein Detection” to learn about getting reproducible western blot results with little hands-on time and using less primary antibody than traditional immunodetection methods.

Play now

Flexible formats

The iBind Flex system comes with interchangable wells which allows you to run multiple membrane formats and even run different primary and secondary antibody conditions in the same device at the same time.

Midi blots

Using the same antibody conditions

Mini blots

Using the same or different antibody conditions

Vertically cut strips

Using the same or different antibody conditions

Results are as good as or better than manually processed western blots

Comparison of western blots processed manually vs. with the iBind Flex Western System. Samples containing GST-tagged recombinant proteins were separated on Invitrogen™ NuPAGE™ 4-12%, 20-well gels in MOPS SDS running buffer and then transferred to nitrocellulose membranes using the iBlot™ 2 Dry Blotting System. Blots were probed with identical concentrations of the same pair of primary and secondary antibodies. The primary antibody was rabbit anti-GST diluted 1:500 (8 µL in 4 mL iBind Flex Solution for the iBind system, 40 µL in 20 mL for manual tray incubation). The secondary antibody was goat anti-rabbit HRP diluted 1:600 (6.7 µL in 4 mL iBind Flex Solution for the iBind system, 33.3 µL in 20 mL for manual tray incubation). For final detection, blots were incubated for 5 minutes in Thermo Scientific™ SuperSignal™ West Dura Substrate for visualization with an imaging system.

Lanes 1-5: IKK beta (80 ng, 40 ng, 20 ng, 10 ng, 5 ng)
Lanes 6-10: DDR2 (120 ng, 60 ng, 30 ng, 15 ng, 7.5 ng)
Lanes 11-15: FLT1 (40 ng, 20 ng, 10 ng, 5 ng, 2.5 ng)
Lanes 16-20: HCK (360 ng, 180 ng, 90 ng, 45 ng, 22.5 ng)

Better western blot results using less primary antibody. Comparison of mini blots processed manually (probing and washing steps performed in a tray) vs. with the Invitrogen™ iBind™ Flex Western Device. Blots were produced by separating samples on Bolt™ 4-12%, 10-well gels with MES SDS running buffer and then transferred to nitrocellulose membranes using the iBlot™ 2 Dry Blotting System. Each gel contained 10 lanes loaded with two-fold dilution series of 293 cell extracts (30 µg to 0.06 µg). After immunodetection using the conditions described below, blots were incubated for 5 minutes in Thermo Scientific™ SuperSignal™ West Dura Substrate and the signal documented with an imager.

  • MEK2 (45 kDa) – rabbit anti-MEK2 antibody applied at 1:1000 for iBind blot processing (= 2 µL in 2 mL of iBind Solution), and 1:1000 for manual blot processing (= 10 µL in 10 mL buffer).
  • Securin(428 kDa) – rabbit anti-Securin antibody applied at 1:1000 for iBind blot processing (= 2 µL in 2 mL of iBind Solution), and 1:1000 for manual blot processing (= 10 µL in 10 mL buffer).
  • Secondary Antibody (both targets): goat anti-rabbit IgG at 1:600 for iBind blot processing (3.33 µL in 2 mL of iBind Solution), and at 1:1800 for manual blot processing (5.55 µL in 10 mL).

Excellent western blot results with vertically cut strips and fluorescence detection. Comparison of mini blots processed manually (probing and washing steps performed in a tray) vs. with the Invitrogen™ iBind™ Flex Western Device. Blots were produced by separating samples on Bolt™ 4-12%, 10-well gels with MES SDS running buffer and then transferred to nitrocellulose membranes using the iBlot™ 2 Dry Blotting System and then cutting each into three-lane strips. Final imaging was performed using the Odyssey™ CLx instrument (LI-COR™, Inc.).

Samples and lanes were as follows:

  • Lane 1:  Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder (3 µL)
  • Strip 1:  Phosphorylated Akt cell extract (15 µg, 7.5 µg, 3.75 µg) and Elk-1 Fusion Protein (150 ng, 75 ng, 37.5 ng)
  • Strip 2:  HeLa cell extract (30 µg, 15 µg, 7.5 µg)
  • Strip 3:  Phosphorylated Akt cell extract (15 µg, 7.5 µg, 3.75 µg)
  • Strip 4:  Elk-1 Fusion Protein (150 ng, 75 ng, 37.5 ng)
  • Strip 5:  HeLa cell extract (30 µg, 15 µg, 7.5 µg)
  • Strip 6:  HeLa cell extract (30 µg, 15 µg, 7.5 µg)

Probing and antibody conditions were as follows:

Strip # 1° Ab [1° Ab] Manual [1° Ab] iBind Flex 2° Ab [2° Ab] Manual [2° Ab] Flex
1 Phospho-Akt (red)
Phospho-Elk-1 (green)
1:2000
1:1000
1:400
1:200
IRDye 680LT
IRDye 800CW
1:20,000
1:15,000
1:4000
1:3000
2 SRC (red)
beta Catenin (green)
1:250
1:1000
1:50
1:200
IRDye 680LT
IRDye 800CW  
1:20,000
1:15,000
1:4000
1:3000
3 Phospho-Akt 1:2000 1:400 IRDye 680LT 1:20,000 1:4000
4 Phospho-Elk-1 1:1000 1:200 IRDye 800CW 1:15,000 1:3000
5 SRC 1:250 1:50 IRDye 680LT 1:20,000 1:4000
6 beta Catenin 1:250 1:200 IRDye 800CW 1:15,000 1:3000

After completion of Western processing, blots were imaged on the Odyssey CLx instrument.

Get your iBind Flex Western System Starter Kit now
 

Begin using a simpler, more elegant western blotting process. The iBind™ Flex Starter Kit includes a complete iBind Flex Western Device, plus cards and a solution kit, so you can get started right away:

  • iBind™ Flex Western Device
  • iBind™ Flex Cards (1 box of 10)
  • iBind™ Flex Solution Kit (Note: fluorescence detection requires FD Solution Kit)
View the user manual:

Sample of available secondary antibodies